scholarly journals In-Vitro Characterization of the Anti-Cancer Activity of the Probiotic Bacterium Lactobacillus Fermentum NCIMB 5221 and Potential against Colorectal Cancer

2015 ◽  
Vol 07 (07) ◽  
Author(s):  
Imen Kahouli Meenakshi
Keyword(s):  
Colorectal Cancer ◽  
Probiotic Bacterium ◽  
Lactobacillus Fermentum ◽  
In Vitro Characterization ◽  
Anti Cancer ◽  
Cancer Activity

In vitro and in vivo anti-cancer activity of tangeretin against colorectal cancer was enhanced by emulsion-based delivery system

2015 ◽  
Vol 15 ◽  
pp. 264-273 ◽  
Author(s):  
Yuwen Ting ◽  
Yi-Shiou Chiou ◽  
Min-Hsiung Pan ◽  
Chi-Tang Ho ◽  
Qingrong Huang
Keyword(s):  
Colorectal Cancer ◽  
Delivery System ◽  
Anti Cancer ◽  
Cancer Activity

In vitro characterization of the human biotransformation pathways of aplidine, a novel marine anti-cancer drug

2006 ◽  
Vol 25 (1) ◽  
pp. 9-19 ◽  
Author(s):  
Esther F. A. Brandon ◽  
Rolf W. Sparidans ◽  
Ronald D. van Ooijen ◽  
Irma Meijerman ◽  
Luis Lopez Lazaro ◽  
...  
Keyword(s):  
Cancer Drug ◽  
In Vitro Characterization ◽  
Anti Cancer

In vitro characterization of the biotransformation of thiocoraline, a novel marine anti-cancer drug

2004 ◽  
Vol 22 (3) ◽  
pp. 241-251 ◽  
Author(s):  
Esther F. A. Brandon ◽  
Rolf W. Sparidans ◽  
Irma Meijerman ◽  
Ignasio Manzanares ◽  
Jos H. Beijnen ◽  
...  
Keyword(s):  
Cancer Drug ◽  
In Vitro Characterization ◽  
Anti Cancer

scholarly journals In Vitro Characterization of the Pharmacological Properties of the Anti-Cancer Chelator, Bp4eT, and Its Phase I Metabolites

PLoS ONE ◽  
2015 ◽  
Vol 10 (10) ◽  
pp. e0139929 ◽  
Author(s):  
Eliška Potůčková ◽  
Jaroslav Roh ◽  
Miloslav Macháček ◽  
Sumit Sahni ◽  
Ján Stariat ◽  
...  
Keyword(s):  
Phase I ◽  
Pharmacological Properties ◽  
In Vitro Characterization ◽  
Anti Cancer

In vitro characterization of the human biotransformation and CYP reaction phenotype of ET-743 (Yondelis®, Trabectidin®), a novel marine anti-cancer drug

2006 ◽  
Vol 24 (1) ◽  
pp. 3-14 ◽  
Author(s):  
Esther F. A. Brandon ◽  
Rolf W. Sparidans ◽  
Kees-Jan Guijt ◽  
Sjoerd Löwenthal ◽  
Irma Meijerman ◽  
...  
Keyword(s):  
Cancer Drug ◽  
In Vitro Characterization ◽  
Anti Cancer

scholarly journals In vitro characterization of the colibactin-activating peptidase ClbP enables development of a fluorogenic activity probe

2019 ◽  
Author(s):  
Matthew R. Volpe ◽  
Matthew R. Wilson ◽  
Carolyn A. Brotherton ◽  
Ethan S. Winter ◽  
Sheila E. Johnson ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Biochemical Characterization ◽  
Active Form ◽  
Inner Membrane ◽  
Substrate Preferences ◽  
In Vitro Characterization ◽  
Catalytically Active ◽  
Membrane Bound

ABSTRACTThe gut bacterial genotoxin colibactin has been linked to the development of colorectal cancer. In the final stages of colibactin’s biosynthesis, an inactive precursor (pre-colibactin) undergoes proteolytic cleavage by ClbP, an unusual inner-membrane-bound periplasmic peptidase, to generate the active genotoxin. This enzyme presents an opportunity to monitor and modulate colibactin biosynthesis, but its active form has not been studied in vitro and limited tools exist to measure its activity. Here, we describe the in vitro biochemical characterization of catalytically active, full-length ClbP. We elucidate its substrate preferences and use this information to develop a fluorogenic activity probe. This tool will enable the discovery of ClbP inhibitors and streamline identification of colibactin-producing bacteria.

Sign in / Sign up

Export Citation Format

Share Document