The Value of Red Blood Cells (Rbc) Indices and Osmotic Fragility Test as Screening Tests in Malay Pregnant Women with Alpha Thalassaemia

Background: The hypoxic environment stimulates the human body to increase the levels of hemoglobin (HGB) and hematocrit and the number of red blood cells. Such enhancements have individual differences, leading to a wide range of HGB in Tibetans’ whole blood (WB). Study Design: WB of male Tibetans was divided into 3 groups according to different HGB (i.e., A: >120 but ≤185 g/L, B: >185 but ≤210 g/L, and C: >210 g/L). Suspended red blood cells (SRBC) processed by collected WB and stored in standard conditions were examined aseptically on days 1, 14, 21, and 35 after storage. The routine biochemical indexes, deformability, cell morphology, and membrane proteins were tested. Results: Mean corpuscular volume, adenosine triphosphate, pH, and deformability were not different in group A vs. those in storage (p > 0.05). The increased rate of irreversible morphology of red blood cells was different among the 3 groups, but there was no difference in the percentage of red blood cells with an irreversible morphology after 35 days of storage. Group C performed better in terms of osmotic fragility and showed a lower rigid index than group A. Furthermore, SDS-PAGE revealed similar cross-linking degrees of cell membrane protein but the band 3 protein of group C seemed to experience weaker clustering than that of group A as detected by Western Blot analysis after 35 days of storage. Conclusions: There was no difference in deformability or morphological changes in the 3 groups over the 35 days of storage. High HGB levels of plateau SRBC did not accelerate the RBC change from a biconcave disc into a spherical shape and it did not cause a reduction in deformability during 35 days of preservation in bank conditions.

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Hemoglobin Oxidation in Stored Blood Accelerates Hemolysis and Oxidative Injury to Red Blood Cells

Journal of Laboratory Physicians ◽

10.1055/s-0040-1721156 ◽

2020 ◽

Vol 12 (04) ◽

pp. 244-249

Author(s):

Ibrahim Mustafa ◽

Tameem Ali Qaid Hadwan

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Morphological Changes ◽

Hemoglobin Concentration ◽

Red Cell Membrane ◽

Rbc Membrane ◽

Hemoglobin Oxidation ◽

Rbc Indices ◽

Methemoglobin Formation ◽

Stored Blood

Abstract Introduction Maintaining blood supply is a challenge in blood banks. Red blood cells (RBCs) stored at 4°C experience issues of biochemical changes due to metabolism of cells, leading to changes collectively referred to as “storage lesions.” Oxidation of the red cell membrane, leading to lysis, contributes to these storage lesions. Methods Blood bags with CPD-SAGM stored at 4°C for 28 days were withdrawn aseptically on days 1, 14, and 28. Hematology analyzer was used to investigate RBC indices. Hemoglobin oxidation was studied through spectrophotometric scan of spectral change. RBC lysis was studied with the help of Drabkin's assay, and morphological changes were observed by light and scan electron microscopy. Results RBCs show progressive changes in morphology echinocytes and spherocytes on day 28. There was 0.85% RBC lysis, an approximately 20% decrease in percentage oxyhemoglobin, and a 14% increase in methemoglobin formation, which shows hemoglobin oxidation on day 28. Conclusions Oxidative damage to RBC, with an increase in storage time was observed in the present study. The observed morphological changes to RBC during the course of increased time shows that there is progressive damage to RBC membrane and a decrease in hemoglobin concentration; percentage RBC lysis is probably due to free hemoglobin and iron.

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HEMOLYTIC DISEASE OF THE NEWBORN DUE TO ANTI-A AND ANTI-B

PEDIATRICS ◽

10.1542/peds.15.1.54 ◽

1955 ◽

Vol 15 (1) ◽

pp. 54-62

Author(s):

Clare N. Shumway ◽

Gerald Miller ◽

Lawrence E. Young

Keyword(s):

B Cells ◽

Red Blood Cells ◽

Blood Group ◽

Newborn Infant ◽

Blood Cells ◽

Osmotic Fragility ◽

Red Cells ◽

Hemolytic Disease ◽

Abo Incompatibility

Ten infants with hemolytic disease of the newborn due to ABO incompatibility were studied. In every case the investigations were undertaken because of jaundice occurring in the first 24 hours of life. The clinical, hematologic and serologic observations in the infants and the serologic findings in the maternal sera are described. Evidence is presented to show that the diagnosis of the disorder rests largely upon the demonstration of spherocytosis, increased osmotic fragility of the red cells, reticulocytosis, and hyperbilirubinemia in a newborn infant whose red blood cells are incompatible with the maternal major blood group isoantibody and against whose cells no other maternal isoantibody is demonstrable. The anti-A or anti-B in each of the maternal sera tested in this series hemolyzed A or B cells in the presence of complement. Other serologic findings in the maternal sera were less consistently demonstrated.

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Alterations in osmotic fragility of the red blood cells in hypo- and hyperthyroid patients

Journal of Endocrinological Investigation ◽

10.1007/bf03345674 ◽

2009 ◽

Vol 32 (1) ◽

pp. 28-32 ◽

Cited By ~ 4

Author(s):

S. Zahedi Asl ◽

N. Khalili Brojeni ◽

A. Ghasemi ◽

F. Faraji ◽

M. Hedayati ◽

...

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Osmotic Fragility ◽

Hyperthyroid Patients

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Determination of Osmotic Fragility of Red Blood Cells

Practical Physiology ◽

10.5005/jp/books/12995_11 ◽

2017 ◽

pp. 64-64

Author(s):

N Geetha

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Osmotic Fragility

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OSMOTIC FRAGILITY OF RED BLOOD CELLS IN SALAMANDERS

Exogenous and Endogenous Influences on Metabolic and Neural Control ◽

10.1016/b978-0-08-028845-1.50122-0 ◽

1982 ◽

pp. 205-206

Author(s):

G. DEGANI

Keyword(s):

Red Blood Cells ◽

Blood Cells ◽

Osmotic Fragility

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Alterations in the Properties of Red Blood Cells in Men with Coronary Artery Diseases after Comprehensive Cardiac Rehabilitation

Cardiology Research and Practice ◽

10.1155/2020/6478785 ◽

2020 ◽

Vol 2020 ◽

pp. 1-9

Author(s):

Krzysztof Gwozdzinski ◽

Anna Pieniazek ◽

Joanna Bernasinska-Slomczewska ◽

Joanna Brzeszczynska ◽

Robert Irzmanski ◽

...

Keyword(s):

Coronary Artery ◽

Membrane Protein ◽

Cardiac Rehabilitation ◽

Red Blood Cells ◽

Membrane Fluidity ◽

Exercise Test ◽

Blood Cells ◽

Reduced Glutathione ◽

Osmotic Fragility ◽

Cardiac Intervention

Purpose. Comprehensive cardiac rehabilitation (CCR) is a complex program aimed at improving the health status of patients with coronary artery disease (CAD), especially those who have been subjected to cardiac interventions (PCI and CABG).The aim of this study was to measure the changes in the properties of red blood cells (RBCs) in men with CAD after cardiac intervention and after participation in CCR program. Methods. In this study, we have investigated the influence of the physical training-based CCR program in 12 men with CAD, after PCI or CABG. The characteristics of RBCs including the basic morphology of RBCs, the conformational state of RBC membrane protein and hemoglobin, acetylcholinesterase activity, membrane fluidity, the osmotic fragility, and thiol concentration in membrane and in hemolysate were measured. Ascorbate concentration and reduced glutathione were also determined. The analysis was performed in men, before and after participation in CCR. The properties of RBCs were observed in connection with the exercise test, and parameters were evaluated before, immediately after, and 1 hour after the exercise test. Results. After CCR, a decrease in the mobility of erythrocyte membrane proteins was observed, which was accompanied by a decrease in lipid fluidity. In addition, immediately after the exercise test and 1 hour later, we measured a decrease in thiol level in hemolysate, but not in the plasma membrane. Unexpectedly, an increase in reduced glutathione concentration one hour after the exercise test after completing comprehensive cardiac rehabilitation was observed. Conclusion. CCR in men with CAD after cardiac intervention is connected with decreased membrane fluidity and decreased membrane protein mobility, which indicates that reduction of oxidative changes in these components occurs.

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