Identification, Mapping and Pyramiding of Genes/Quantitative Trait Loci (QTLs) for Durable Resistance of Crops to Biotic Stresses

Management of oat crown rust disease with host resistance is challenging because major gene resistance is generally short lived. Partially resistant oat cultivars could benefit oat growers by providing more durable resistance. The objective of this study was to validate and discover quantitative trait loci (QTL) affecting crown rust resistance in the partially resistant oat line MN841801-1 using conventional and molecular assessments of disease produced in single-race greenhouse inoculations, single-race polycyclic field tests, and under natural infection in disease-conducive environments. Crown rust was assessed on 150 F6:9 MN841801-1/‘Noble-2’ recombinant inbred lines. In total, eight QTL associated with MN841801-1 alleles were detected. Of these, seven matched QTL previously identified while a new QTL (Prq8) was detected on linkage group MN13. Four QTL (Prq1a, Prq2, Prq7, and Prq8) were consistently detected and predicative genetic assays for these QTL should be developed for future validation in additional genetic backgrounds.

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A Genome-Wide Meta-Analysis of Rice Blast Resistance Genes and Quantitative Trait Loci Provides New Insights into Partial and Complete Resistance

Molecular Plant-Microbe Interactions ◽

10.1094/mpmi-21-7-0859 ◽

2008 ◽

Vol 21 (7) ◽

pp. 859-868 ◽

Cited By ~ 216

Author(s):

Elsa Ballini ◽

Jean-Benoît Morel ◽

Gaétan Droc ◽

Adam Price ◽

Brigitte Courtois ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Resistance Genes ◽

Quantitative Trait ◽

Rice Blast ◽

Blast Resistance ◽

Durable Resistance ◽

Rice Genome ◽

Blast Disease ◽

Trait Loci ◽

Blast Resistance Genes

The completion of the genome sequences of both rice and Magnaporthe oryzae has strengthened the position of rice blast disease as a model to study plant–pathogen interactions in monocotyledons. Genetic studies of blast resistance in rice were established in Japan as early as 1917. Despite such long-term study, examples of cultivars with durable resistance are rare, partly due to our limited knowledge of resistance mechanisms. A rising number of blast resistance genes and quantitative trait loci (QTL) have been genetically described, and some have been characterized during the last 20 years. Using the rice genome sequence, can we now go a step further toward a better understanding of the genetics of blast resistance by combining all these results? Is such knowledge appropriate and sufficient to improve breeding for durable resistance? A review of bibliographic references identified 85 blast resistance genes and approximately 350 QTL, which we mapped on the rice genome. These data provide a useful update on blast resistance genes as well as new insights to help formulate hypotheses about the molecular function of blast QTL, with special emphasis on QTL for partial resistance. All these data are available from the OrygenesDB database.

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Genes/Quantitative Trait Loci and Associated Molecular Mechanisms Identified in Capsicum Genome for Tolerance to Abiotic and Biotic Stresses

Compendium of Plant Genomes - The Capsicum Genome ◽

10.1007/978-3-319-97217-6_7 ◽

2019 ◽

pp. 121-138 ◽

Cited By ~ 1

Author(s):

Vandana Jaiswal ◽

Vijay Gahlaut ◽

Meenakshi Dubey ◽

Nirala Ramchiary

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Molecular Mechanisms ◽

Abiotic And Biotic Stresses ◽

Biotic Stresses ◽

Trait Loci

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PERKEMBANGAN DAN TANTANGAN PERAKITAN VARIETAS TAHAN DALAM PENGENDALIAN WERENG COKLAT DI INDONESIA

Jurnal Penelitian dan Pengembangan Pertanian ◽

10.21082/jp3.v34n4.2015.p187-193 ◽

2016 ◽

Vol 34 (4) ◽

pp. 187

Author(s):

Eko Hari Iswanto ◽

Untung Susanto ◽

Ali Jamil

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Marker Assisted Selection ◽

Durable Resistance ◽

Trait Loci ◽

Rathu Heenati

Varietas tahan merupakan komponen utama dalam pengendalian wereng coklat pada tanaman padi. Namun, varietas tahan yang ditanam secara luas dan terus-menerus dapat patah ketahanannya karena wereng coklat mampu beradaptasi dengan membentuk biotipe baru. Saat ini lebih dari 70 gen ketahanan terhadap wereng, baik gen utama maupun quantitative trait loci (QTLs), telah diidentifikasi dan beberapa gen telah digunakan dalam perakitan varietas unggul padi. Keanekaragaman sumber gen ketahanan pada varietas dapat menjadi alternatif pergiliran tanaman dalam upaya meredam serangan wereng coklat. Tantangan perakitan varietas tahan ke depan ialah produktivitas tinggi dan toleran terhadap cekaman abiotik. Perakitan varietas tahan dengan ketahanan lestari (durable resistance) menggunakan marker-assisted selection (MAS) dengan menggabungkan lebih dari dua gen ketahanan (gen utama maupun QTLs) diharapkan dapat menghambat pembentukan biotipe baru wereng coklat. Gen-gen ketahanan pada beberapa varietas diferensial seperti Rathu Heenati (Bph3 dan Bph17) dan PTB33 (bph2 dan Bph3) masih mempunyai ketahanan yang baik terhadap populasi wereng coklat dari daerah endemis dan dapat dijadikan donor dalam perakitan varietas tahan. Deteksi gen ketahanan pada varietas unggul padi yang akan dilepas sangat penting untuk menentukan rekomendasi daerah/lokasi tanam dan pergiliran varietas. Selain itu, manajemen ketahanan varietas juga penting agar varietas tahan yang dilepas dapat bertahan lama di lapangan.

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Mapping of Quantitative Trait Loci for Scab Resistance in Apple (Malus × Domestica)

10.21203/rs.3.rs-1003617/v1 ◽

2021 ◽

Author(s):

Saba Mir ◽

Aafreen Sakina ◽

Khalid Z. Masoodi ◽

Khalid M. Bhat ◽

Bilal A. Padder ◽

...

Keyword(s):

Quantitative Trait Loci ◽

Quantitative Trait ◽

Venturia Inaequalis ◽

Durable Resistance ◽

Genetic Resistance ◽

In Silico Analysis ◽

Genomic Region ◽

Scab Resistance ◽

Chromosome 2 ◽

Trait Loci

Abstract Scab caused by Venturia inaequalis (Cke.) Wint. is the most important fungal disease of apple. Fungicide application is a widely practiced method of disease control. The use of chemicals is however, cost intensive, tedious and ecologically unsafe. Development of genetic resistance and breeding of resistant cultivars is most reliable and a safest option. One such source of scab resistance happens to be the variety ‘Shireen’, released from SKUASTKashmir. However, till date the nature of resistance and its genetic control has not been characterized. The aim of this research was to elucidate the genetic basis of scab resistance in Shireen. The present study helped us to identify two quantitative trait loci (QTLs) on chromosome 2 and 8 and six potential CDGs for the polygenic resistance in ‘Shireen’. The genomic region corresponding to the mapped QTLs in LG 2 and LG 8 of ‘Shireen’ was examined for candidate genes possibly related to scab resistance using in silico analysis. The QTLs mapped in the genetic background of Shireen are the novel QTLs and may be transferred to desirable genetic backgrounds and provide opportunities for isolation and cloning of genes apart from their utility in order to achieve durable resistance to scab.

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