scholarly journals The Spleen Contributes Stem Cells to Peripheral Blood Stem Cell Transplants

Author(s):  
Toshiyuki Mera
1998 ◽  
Vol 16 (2) ◽  
pp. 610-615 ◽  
Author(s):  
M J Egorin ◽  
D M Rosen ◽  
R Sridhara ◽  
L Sensenbrenner ◽  
M Cottler-Fox

PURPOSE Dimethylsulfoxide (DMSO) is used to cryopreserve hematopoietic stem cells and is obligatorily infused into patients who receive stem-cell transplants. This study characterized the plasma concentrations and pharmacokinetics of DMSO and its metabolites in patients who underwent peripheral-blood stem-cell transplants. MATERIALS AND METHODS Plasma concentrations of DMSO, dimethylsulfone (DMSO2), and dimethylsulfide (DMSH2) were assessed in 10 patients who underwent autologous transplants with stem cells, cryopreserved in 10% DMSO (vol/vol). Blood was sampled at multiple times after the stem-cell infusion. Urine was pooled during the 24 hours postinfusion. DMSO, DMSO2, and DMSH2 were assayed simultaneously by gas chromatography. A one-compartment model with saturable elimination proved most suitable for fitting plasma DMSO concentration-versus-time data. RESULTS Stem-cell volumes infused ranged between 180 and 585 mL (254 to 824 mmol DMSO). Infusions lasted between 20 and 120 minutes. Peak plasma DMSO concentrations were 19.1 +/- 6.3 mmol/L (mean +/- SD). Pharmacokinetic parameters for volume of the central compartment (Vc), maximum velocity (Vmax), and Michaels-Menten constant (Km) were 37.3 +/- 17 L, 0.99 +/- 0.57 mmol/L/h, and 5.2 +/- 5.0 mmol/L, respectively. Plasma DMSO2 concentrations increased during the first 24 hours, plateaued at 4.4 +/- 1.2 mmol/L, and remained there until 48 hours (the last sample). DMSH2 concentrations were at steady-state by 5 minutes and remained between 3 and 5 mmol/L for 48 hours. Urinary excretion of DMSO and DMSO2 accounted for 44% +/- 4% and 4% +/- 1%, respectively, of the administered DMSO dose. Renal clearance of DMSO was 14.1 +/- 3.4 mL/min. CONCLUSION These data (1) document plasma concentrations of DMSO and metabolites in patients following peripheral-blood stem-cell transplants; (2) allow consideration of potential effects of these concentrations on stem-cell engraftment and drug-drug interactions; and (3) can facilitate a concentration-guided phase I trial of DMSO.


2013 ◽  
Vol 99 (2) ◽  
pp. 526-532.e2 ◽  
Author(s):  
Erin F. Wolff ◽  
Naoya Uchida ◽  
Robert E. Donahue ◽  
Mark E. Metzger ◽  
Matthew M. Hsieh ◽  
...  

1999 ◽  
Vol 23 (4) ◽  
pp. 335-346 ◽  
Author(s):  
S Shenoy ◽  
T Mohanakumar ◽  
G Todd ◽  
W Westhoff ◽  
K Dunnigan ◽  
...  

2001 ◽  
Vol 27 (2) ◽  
pp. 201-205 ◽  
Author(s):  
G Aksu ◽  
MZ Ruhi ◽  
H Akan ◽  
S Bengisun ◽  
C Üstün ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document