scholarly journals Current Status of <i>Withania somnifera</i> (L.) Dunal: An Endangered Medicinal Plant from Himalaya

2017 ◽  
Vol 08 (05) ◽  
pp. 1159-1169 ◽  
Author(s):  
Sumaira Aslam ◽  
Naveed Iqbal Raja ◽  
Mubashir Hussain ◽  
Muhammad Iqbal ◽  
Muhammad Ejaz ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
Current Status ◽  
Endangered Medicinal Plant

Plant Regeneration of Withania somnifera (L.) Dunal (Ashwagandha) from Nodal Segments Derived Callus an Endangered Medicinal Plant in Bangladesh

2004 ◽  
Vol 4 (2) ◽  
pp. 219-223 ◽  
Author(s):  
N.A. Siddique . ◽  
M.A. Bari . ◽  
Sharmin Shahnewaz . ◽  
M.H. Rahman . ◽  
M.R. Hasan . ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Medicinal Plant ◽  
Withania Somnifera ◽  
Nodal Segments ◽  
Endangered Medicinal Plant

scholarly journals In vitro Regeneration of an Endangered Medicinal Plant Withania somnifera using Four Different Explants

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
A. Arumugam ◽  
K. Gopinath
Keyword(s):  
Medicinal Plant ◽  
Withania Somnifera ◽  
In Vitro Regeneration ◽  
Multiple Shoots ◽  
Leaf Explant ◽  
Cow Dung ◽  
Endangered Medicinal Plant ◽  
Rooting Medium ◽  
Regenerated Plantlets

An efficient protocol was developed for highly regenerative capacity from leaf explant of Withania somnifera (L.) Dunal ? an endangered medicinal plant. Calli were regenerated from four different explants like leaves, cotyledons, hypocotyls and epicotyls. MS supplemented with different concentrations of 2,4?D, BAP and NAA were used. The calli (94.33 ± 1.20%) were obtained from the leaf explant in 2,4?D 3.0 mg/l. The highest number of multiple shoots (85.67 ± 0.88%) were obtained from the leaf callus at 4.0 mg/l BAP. Shootlets forming calli were transferred to the rooting medium containing 10.0 mg/l NAA to produce multiple roots (89.33 ± 0.88%). The regenerated rooted shootlets were transferred to small polythene bags, which contain a sterilized cow?dung, sand and red soil (1 : 2 : 3) and kept in a mist house. After acclimation in the mist house the regenerated plantlets were hardened in the greenhouse and transferred to soil, which showed 85% survival rate. This new protocol was standardized for easy mass propagation of W. somnifera using leaf explant.Plant Tissue Cult. & Biotech. 23(1): 79?85, 2013 (June)DOI: http://dx.doi.org/10.3329/ptcb.v23i1.15564

scholarly journals Seed germination requirements of Hypericum scruglii, an endangered medicinal plant species of Sardinia (Italy)

Botany ◽  
2020 ◽  
Vol 98 (10) ◽  
pp. 615-621 ◽  
Author(s):  
Marco Porceddu ◽  
Martina Sanna ◽  
Sara Serra ◽  
Maria Manconi ◽  
Gianluigi Bacchetta
Keyword(s):  
Medicinal Plant ◽  
Seed Bank ◽  
Seed Viability ◽  
Base Temperature ◽  
Endemic Plant ◽  
Endangered Medicinal Plant ◽  
High Viability ◽  
Wide Range ◽  
Baseline Information ◽  
Germination Requirements

Hypericum scruglii Bacch., Brullo & Salmeri is an endangered endemic plant of Sardinia, and the phloroglucinol compounds identified in this species have been reported to inhibit the activity of the Human Immunodeficiency Virus. Seed banks are a genetic repository that effectively preserve taxa of conservation interest, and they hold knowledge about the biology and germination ecophysiology of the taxa they preserve. The main goals of this study were to investigate the germination requirements for H. scruglii, to evaluate the seed viability after eight years of long-term conservation and to suggest an efficient protocol for germination. Seeds stored at –25 °C in the seed bank were tested at temperatures from 5 to 30 °C and 25/10 °C. Base temperature (Tb) and thermal time (θ50) for germination were estimated. Seeds of H. scruglii germinated over a wide range of temperatures, responding positively to high temperatures and 25/10 °C. Tb was estimated at 4.92 °C, and θ50 was estimated at 198.27 degree days. The high viability detected in seeds of H. scruglii allows us to suggest the use of seeds stored in a seed bank when fresh material for plant propagation is scarce. Our results provide new and useful baseline information for implementing conservation and multiplication strategies for this endangered medicinal plant.

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