Study Biocompatibility and Osteogenic Differentiation Potential of Human Umbilical Cord Mesenchymal Stem Cells (hUCMSCs) with Gelatin Solvent

Induced osteogenesis of mesenchymal stem cells (MSCs) may provide an important tool for bone injures treatment. Human umbilical cord and adipose tissue are routinely discarded as clinical waste and may be used as uncontroversial MSCs sources. It still remains to be verified which source of MSCs is the most suitable for bone regeneration.The aim of this research was to investigate the osteogenic potential of human MSCs derived from adipose tissue (ASCs) and Wharton’s jelly of the human umbilical cord (WJ-MSCs) differentiated under the same conditions.Osteogenic differentiation of MSCs was detected and quantified by ARS staining for calcium deposition and alkaline phosphatase (ALP) activity, osteoprotegerin (OPG), and osteocalcin (OC) secretion measurements. Under osteogenic conditions the measured ALP activity and calcium deposition were significantly higher in ASCs than in WJ-MSCs, while the OPG and OC secretion were higher in WJ-MSCs vs. ASCs. Low concentrations of OPG and high levels of OC in ASCs and WJ-MSCs, prove that these cells reached an advanced stage of the osteogenic differentiation. The levels of OC secreted by ASCs were lower than by WJ-MSCs what indicates that the differentiation process of the ASCs reached the stage when the extracellular matrix is overproduced and the down-regulation of OC begins.Both cell types, ASCs and WJ-MSCs possess potential to differentiate towards the osteogenic lineage. However, the observed differences in the levels of osteogenic markers suggest that ASCs may be better candidates for cell-based osteogenesis than WJ-MSCs.

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Static magnetic field-enhanced osteogenic differentiation of human umbilical cord-derived mesenchymal stem cells via matrix vesicle secretion

International Journal of Radiation Biology ◽

10.1080/09553002.2020.1787545 ◽

2020 ◽

Vol 96 (9) ◽

pp. 1207-1217

Author(s):

Ching-Yi Chang ◽

Wei-Zhen Lew ◽

Sheng-Wei Feng ◽

Chung-Lung Wu ◽

Hsin-Hui Wang ◽

...

Keyword(s):

Magnetic Field ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Osteogenic Differentiation ◽

Umbilical Cord ◽

Static Magnetic Field ◽

Matrix Vesicle ◽

Human Umbilical Cord ◽

Vesicle Secretion

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Evaluation of isolation method in remaining of differentiation potential of perivascular human umbilical cord mesenchymal stem cells toward male germ cell-like

Journal of Shahrekord University of Medical Sciences ◽

10.34172/jsums.2021.13 ◽

2021 ◽

Vol 23 (2) ◽

pp. 81-86

Author(s):

Ali Shojaeian ◽

Ameneh Mehri-Ghahfarrokhi ◽

Shima Rahmati-Dehkordi ◽

Mehdi Banitalebi-Dehkordi

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Germ Cell ◽

Umbilical Cord ◽

Adult Stem Cells ◽

Male Germ Cell ◽

Human Umbilical Cord ◽

Differentiation Potential ◽

Protein Levels ◽

Old Mice

Background and aims: Infertility is one of the most common problems among couples. Generation of male germ cells from adult stem cells is a current promising priority of researchers. This study aimed to investigate the potential of human umbilical cord mesenchymal stem cells (hUMSCs) on the expression of male germ cell markers after isolating by this method. Methods: The hUMSCs was incubated with retinoic acid, testosterone, and conditioned medium (prepared from testicular cell cultures of 7-day-old mice) during 3 days. The bands were visualized and densitometry was accomplished using LI-COR Biosciences software. Results: The high expression levels of C-KIT, DAZL, PIWIL2, and DDX4 in mRNA and protein levels were observed in treated hUMSCs. Conclusion: Results of reverse transcription polymerase chain reaction (RT-PCR) and western blotting showed that method of isolation had no adverse effects on differentiation potential of hUMSCs.

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Differentiation of Human Umbilical Cord Derived Mesenchymal Stem Cells Into Neural Stem Cells Induced by hPRDX5

10.21203/rs.3.rs-1061159/v1 ◽

2021 ◽

Author(s):

Yuanyuan Jin ◽

Beichen Shi ◽

Qiang Fan ◽

Kun Liu ◽

Shuai Fan ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Neural Stem Cells ◽

Umbilical Cord ◽

Expression Profiles ◽

Antigen Expression ◽

Culture Method ◽

Human Umbilical Cord ◽

Differentiation Potential ◽

Surface Antigen Expression

Abstract Background: The objectives of this study were to investigate the characteristics and capacity of human umbilical cord‑derived mesenchymal stem cells (hUC-MSCs) differentiation into neural stem cells (NSCs) and whether this event enhanced by hPRDX5. Methods and Results: The adherent cells were obtained from umbilical cord of normal full-term newborn by caesarean section under aseptic condition, and cultivated by tissue block culture method. The surface antigen expression profiles of hUC-MSCs were monitored and the multi-directional differentiation potential was identified. Following amplification, the cells of the 4th passage were divided into 5 groups (groups A-E). The morphology was observed under inverted microscope, and the positive expression rate of markers of neural stem cell was detected by immunocytochemical and western blot. Flow cytometry revealed that the hUC-MSCs expressed CD29, CD73, CD90 and CD105, but not CD19, CD34, CD45 or HLA-DR. Treatment with hPRDX5 led to the surface markers of neural stem cells which were positive for Nestin, but negative for NSE and GFAP expression. Conclusions: Thus, the findings of the present study demonstrate that hPRDX5 effectively promotes hUC-MSCs to differentiate into neural stem cells possibly through TLR4 signaling pathway.

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