An Experimental Study on the Effects of Platelet Rich Plasma on the Wound Healing of Tooth Extraction-Related Bone Defects

Platelet-rich plasma (PRP) is widely used in human medicine, but seldom used in veterinary practice. Even so, the efficacy of PRP in osteoarthritis, dental implants, bone defects and wound healing in dogs and horses is well documented. There are many commercially available PRP tubes and kits for horses and humans, but studies on the effectiveness of these products in dogs are limited. Researches have demonstrated that systems developed for humans and horses do not always produce the same results in dogs. In the present study, two prevailing systems, a sodium citrate-based system and a gel-based system, were compared in terms of their platelet output. The data obtained revealed that, although there was no statistically significant difference between the two systems, the sodium citrate-based kit is probably more efficient in practice due to its superiority in volume output. PRP is an important component of regenerative therapy. It is autologous and practical, but has not been sufficiently implemented in veterinary practice, but promising clinical satisfaction and must be considered in various conditions.

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The effect of intrastromal PRP and oral doxycycline in corneal wound healing after alkali burn

Journal of the Hellenic Veterinary Medical Society ◽

10.12681/jhvms.16119 ◽

2019 ◽

Vol 69 (4) ◽

pp. 1213 ◽

Cited By ~ 1

Author(s):

Glykeria Charalambidou ◽

Evangelia Kofidou ◽

Nikolaos Papaioannou ◽

Vasileios Karampatakis ◽

Anastasia Komnenou

Keyword(s):

Experimental Study ◽

Wound Healing ◽

Platelet Rich Plasma ◽

Autologous Blood ◽

Healing Process ◽

Antimicrobial Properties ◽

Control Group ◽

Chemical Burns ◽

Oral Doxycycline ◽

Group 4

Ocular chemical burns are among the most critical and true ocular emergencies in dogs and cats, with destructive consequences on the ocular surface and intraocular tissues. Platelet alpha granules are an important reservoir of growth factors that can stimulate chemotaxis of inflammatory cells, mitosis, migration and differentiation of cells. On the other hand, tetracyclines and their derivatives exhibit non-antimicrobial properties, such as affecting inflammation, immunomodulation, cell proliferation and angiogenesis. The objective of this study is to investigate the effect of intrastromal platelet rich plasma (PRP) in combination with oral doxycycline in the healing process of the cornea. Thirty six New Zealand rabbits were used in this experimental study. Alkali burns were created by applying a round filter paper soaked in NaOH. PRP was prepared by centrifugation of autologous blood. The rabbits were allocated to four groups (9 animals each). Group 1 served as the control group without any treatment. Group 2 received doxycycline (5mg/kg b.w.) orally once a day throughout the study. Group 3 received an intrastromal injection of PRP (0.5ml) in the cornea, and group 4 received intrastromal PRP combined with oral doxycycline. Treatments had varied levels of success, with PRP combined with oral doxycycline producing the best results regarding corneal healing. Animals in the control group had epithelial defects for the whole duration of the study, and 3 animals presented corneal perforation. Groups 3 and 4 had smaller mean defect area, compared to groups 1 and 2. On the 7th day, neovascularisation was lower in treatment groups compared to the control group. Groups 3 and 4 also had less corneal oedema compared to the control group on day 3 and 7. Group 4 exhibited the best wound healing, with less neovascularization and better collagen arrangement, as shown by the histopathological evaluation. To the authors’ knowledge this is the first experimental study that intrastromal PRP is combined with oral doxycycline for the management of corneal chemical burns. This combination is a simple, safe and economical therapeutic approach that promotes corneal healing.

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