scholarly journals Characterization of a mesenchymal stem cell line that differentiates to bone and provides niches supporting mouse and human hematopoietic stem cells

2012 ◽  
Vol 02 (01) ◽  
pp. 5-14 ◽  
Author(s):  
Sonal R. Tuljapurkar ◽  
John D. Jackson ◽  
Susan K. Brusnahan ◽  
Barbara J. O’Kane ◽  
John G. Sharp
2010 ◽  
Vol 37 (7) ◽  
pp. 475-482 ◽  
Author(s):  
Jiao Gao ◽  
Xin-Long Yan ◽  
Ren Li ◽  
Yi Liu ◽  
Wenyan He ◽  
...  

2016 ◽  
Vol 10 (1) ◽  
pp. 10-17 ◽  
Author(s):  
M. A. Shilina ◽  
A. P. Domnina ◽  
I. V. Kozhuharova ◽  
V. V. Zenin ◽  
S. V. Anisimov ◽  
...  

2009 ◽  
Vol 2009 ◽  
pp. 1-10 ◽  
Author(s):  
G. Forte ◽  
O. Franzese ◽  
S. Pagliari ◽  
F. Pagliari ◽  
A. M. Di Francesco ◽  
...  

An immortalized murine mesenchymal stem cell line (mTERT-MSC) enriched forLinneg/Sca-1posfraction has been obtained through the transfection of MSC with murine TERT and single-cell isolation. Such cell line maintained the typical MSC self-renewal capacity and continuously expressed MSC phenotype. Moreover, mTERT-MSC retained the functional features of freshly isolated MSC in culture without evidence of senescence or spontaneous differentiation events. Thus, mTERT-MSC have been cultured onto PLA films, 30 and 100 μm PLA microbeads, and onto unpressed and pressed HYAFF-11 scaffolds. While the cells adhered preserving their morphology on PLA films, clusters of mTERT-MSC were detected on PLA beads and unpressed fibrous scaffolds. Finally, mTERT-MSC were not able to colonize the inner layers of pressed HYAFF-11. Nevertheless, such cell line displayed the ability to preserve Sca-1 expression and to retain multilineage potential when appropriately stimulated on all the scaffolds tested.


BMB Reports ◽  
2010 ◽  
Vol 43 (12) ◽  
pp. 789-794
Author(s):  
Mingke Wang ◽  
Huiqin Sun ◽  
Fan Jiang ◽  
Jing Han ◽  
Feng Ye ◽  
...  

2017 ◽  
Vol 7 (4) ◽  
pp. 531-546 ◽  
Author(s):  
Hamze Timari ◽  
Karim Shamsasenjan ◽  
Aliakbar Movassaghpour ◽  
Parvin Akbarzadehlaleh ◽  
Davod Pashoutan Sarvar ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document