The Effect of Mesenchymal Stem Cell-Derived Extracellular Vesicles on Hematopoietic Stem Cells Fate

The genetic elements that govern the differentiation and proliferation of hematopoietic stem cells remain to be defined. We describe here marked strain-specific differences in the frequency of long-term culture-initiating cells (LTC-IC) in the bone marrow of different strains of mice. Mice of C57Bl/6 background showed the lowest levels of stem cells in marrow, averaging 2.4 +/- .06 LTC-IC/10(5) cells, BALB/c is intermediate (9.1 +/- 4.2/10(5) cells), and DBA/2 mice contained a 11-fold higher frequency of LTC-IC (28.1 +/- 16.5/10(5) cells) than C57Bl/6 mice. The genetic factors affecting the size of the stem cell pool were analyzed in the C57Bl/6 X DBA/2 recombinant inbred strains; LTC-IC frequencies ranged widely, indicating that stem cell frequencies are controlled by multiple genes. Quantitative trait linkage analysis suggested that two loci that have major quantitative effects are located on chromosome 1 near Adprp and Acrg, respectively. The mapping of the locus near Adprp was confirmed by finding an elevated stem cell frequency in B6.C-H25, a C57Bl/6 congenic strain that carries a portion of chromosome 1 derived from BALB/c mice. We have named this gene Scfr1 (stem cell frequency regulator 1). The allelic forms of this gene may be an important predictor of stem cell number and thus would be useful for evaluating cell sources in clinical stem cell transplantation.

Download Full-text

Role of Stem Cell Niche in the Maintenance of Hematopoietic Stem Cells

Inflammation and Regeneration ◽

10.2492/inflammregen.27.117 ◽

2007 ◽

Vol 27 (2) ◽

pp. 117-123 ◽

Cited By ~ 1

Author(s):

Fumio Arai ◽

Toshio Suda

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Hematopoietic Stem Cells ◽

Stem Cell Niche ◽

Hematopoietic Stem ◽

Cell Niche

Download Full-text

Selected Presentations from the Meeting "Isolation of Human Hematopoietic Stem Cells, Stem Cell Expansion, and the Application of New Growth Factors" Held in St. Louis, Missouri, October 3, 1992

Journal of Hematotherapy ◽

10.1089/scd.1.1993.2.111 ◽

1993 ◽

Vol 2 (1) ◽

pp. 111-114 ◽

Cited By ~ 1

Author(s):

R.G. Andrews ◽

E.M. Bryant ◽

D.Y. Muirhead ◽

S.H. Bartelmez ◽

W. Bensinger ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Growth Factors ◽

Hematopoietic Stem Cells ◽

Cell Expansion ◽

Hematopoietic Stem ◽

Stem Cell Expansion

Download Full-text

CD34+ Hematopoietic Stem Cells Exert Accessory Function in Lipopolysaccharide-induced T Cell Stimulation and CD80 Expression on Monocytes

Journal of Experimental Medicine ◽

10.1084/jem.189.4.693 ◽

1999 ◽

Vol 189 (4) ◽

pp. 693-700 ◽

Cited By ~ 15

Author(s):

Taila Mattern ◽

Gundolf Girroleit ◽

Hans-Dieter Flad ◽

Ernst T. Rietschel ◽

Artur J. Ulmer

Keyword(s):

Stem Cells ◽

T Cells ◽

Stem Cell ◽

T Cell ◽

Hematopoietic Stem Cells ◽

Cell Stimulation ◽

Hematopoietic Stem ◽

Accessory Function ◽

T Cell Stimulation ◽

Blood Stem Cells

CD34+ hematopoietic stem cells, which circulate in peripheral blood with very low frequency, exert essential accessory function during lipopolysaccharide (LPS)-induced human T lymphocyte activation, resulting in interferon γ production and proliferation. In contrast, stimulation of T cells by “conventional” recall antigens is not controlled by blood stem cells. These conclusions are based on the observation that depletion of CD34+ blood stem cells results in a loss of LPS-induced T cell stimulation as well as reduced expression of CD80 antigen on monocytes. The addition of CD34-enriched blood stem cells resulted in a recovery of reactivity of T cells and monocytes to LPS. Blood stem cells could be replaced by the hematopoietic stem cell line KG-1a. These findings may be of relevance for high risk patients treated with stem cells or stem cell recruiting compounds and for patients suffering from endotoxin-mediated diseases.

Download Full-text