<p>Genetic Diversity Analysis of Jatropha Curcas<br />Provenances Using Randomly Amplified Polymorphic<br />DNA Markers. Dani Satyawan and I Made Tasma.<br />Jatropha curcas nuts are rich in oil that is higly suitable for<br />Hak Cipta © 2011, BB-Biogen<br />the production of bio-diesel or to be used directly in<br />modified diesel engines. The objective of this study was to<br />assess the extent of genetic diversity among 50 J. curcas<br />provenances and one accession of J. integerrima using<br />RAPD markers. The fifty J. curcas provenances were<br />collected from ecologically diverse regions of Indonesia, and<br />planted in the Pakuwon Experimental Station (Sukabumi,<br />West Java). Fourteen RAPD primers with 60-80% G+C<br />content were used in this genetic diversity analysis and<br />produced 64 bands with 95.7% polymorphism level. The<br />Polymerase Chain Reactions used to generate the RAPD<br />bands sometimes produced inconsistent and nonreproducible<br />results, necessitating the duplication of each<br />reaction to prevent scoring errors. Sixty one validated bands<br />were subsequently used for genetic diversity analysis using<br />Unweighted Pair Group Method Arithmetic (UPGMA)<br />method and Dice coefficients. It was shown that the<br />similarity coefficients among the provenances ranged from<br />0.2 to 0.98 with an average similarity of 0.75. Dendrogram<br />analysis produced two major groups of provenances, with<br />one outlier from South Lampung. There was no tendency for<br />provenances originated from nearby regions to cluster<br />together in each group, and several provenances showed<br />more similarities with provenances originated from distant<br />regions. This pattern lent credence to reports that Jatropha<br />was introduced to Indonesia around four centuries ago and<br />was mainly spread by humans. Based on the mean<br />similarities among the accessions and their clustering<br />pattern, the genetic diversity of the Jatropha collection<br />appeared to be fairly low. Future additions of genetic<br />materials from more diverse genetic background will be<br />necessary to maintain the current progress of Jatropha<br />improvement program.</p>
Genetic diversity analysis of Jatropha curcas L. (Euphorbiaceae) based on methylation-sensitive amplification polymorphism
