scholarly journals Comparative application of direct sequencing, PCR-RFLP, and cytogenetic markers in the genetic characterization of Pimelodus (Siluriformes: Pimelodidae) species: Possible implications for fish conservation

2014 ◽  
Vol 13 (2) ◽  
pp. 4529-4544 ◽  
Author(s):  
M. Ferreira ◽  
K.C.O. Bressane ◽  
A.R.C. Moresco ◽  
O. Moreira-Filho ◽  
L.F. Almeida-Toledo ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Direct Sequencing ◽  
Fish Conservation ◽  
Pcr Rflp

scholarly journals Genetic Characterization of Thyroglobulin and Leptin Genes in Pasundan Cattle at West Java

2019 ◽  
Vol 43 (1) ◽  
Author(s):  
Widya Pintaka Bayu Putra ◽  
Saiful Anwar ◽  
Syahruddin Said ◽  
Romanos Albert Adhitya Indarto ◽  
Putri Wulandari
Keyword(s):  
Genetic Characterization ◽  
Rare Allele ◽  
Basic Information ◽  
Dominant Allele ◽  
West Java ◽  
Pcr Rflp ◽  
Molecular Selection ◽  
Lep Gene ◽  
Informative Content

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.

scholarly journals USE OF PCR-RFLP FOR GENETIC CHARACTERIZATION OF ECHINOCOCCUS GRANULOSUSISOLATES FROM EGYPT

2020 ◽  
Vol 71 (1) ◽  
pp. 249-257
Author(s):  
Doaa Nassar ◽  
Hala Elwakil ◽  
Hayam Ezz Eldin ◽  
Hanan Abou-Seri ◽  
Khalifa Khalifa
Keyword(s):  
Genetic Characterization ◽  
Pcr Rflp

scholarly journals Occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in slaughtered domestic rabbits in central China

Parasite ◽  
2019 ◽  
Vol 26 ◽  
pp. 36 ◽  
Author(s):  
Weifeng Qian ◽  
Wenchao Yan ◽  
Chaochao Lv ◽  
Rongzhen Bai ◽  
Tianqi Wang
Keyword(s):  
Toxoplasma Gondii ◽  
Genetic Characterization ◽  
Neospora Caninum ◽  
Central China ◽  
Occurrence Rate ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Parasite Infections ◽  
Domestic Rabbits

Currently, information on the occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in tissues of rabbits in China is lacking. In this study, brain and heart samples from 470 slaughtered domestic rabbits were collected in Henan Province, Central China. The occurrence rate of T. gondii and N. caninum DNA detected by nested PCR was 2.8% and 2.1%, respectively. There were no significant differences (p > 0.05) in the frequency of the two parasite infections in relation to sex, breed, and region. Three out of 13 T. gondii-positive samples were completely or partially genotyped at 11 genetic markers using PCR-RFLP, and one was identified as ToxoDB genotype #9. For N. caninum, three different sequences at the ITS1 region and two genotypes at the MS5 microsatellite locus were identified. To our knowledge, this is the first genetic characterization of N. caninum isolates from rabbits.

Molecular genetic characterization of new bovine kappa-casein alleles CSN3F and CSN3G and genotyping by PCR-RFLP

2009 ◽  
Vol 27 (5) ◽  
pp. 347-349 ◽  
Author(s):  
E-M Prinzenberg ◽  
S Hiendleder ◽  
T Ikonen ◽  
G Erhardt ◽  
E-M Prinzenberg ◽  
...  
Keyword(s):  
Genetic Characterization ◽  
Molecular Genetic ◽  
Pcr Rflp ◽  
Molecular Genetic Characterization

scholarly journals Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165308 ◽  
Author(s):  
Wen-Bin Zheng ◽  
Xiao-Xuan Zhang ◽  
Jian-Gang Ma ◽  
Fa-Cai Li ◽  
Quan Zhao ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Molecular Detection ◽  
Genetic Characterization ◽  
Northern China ◽  
Neovison Vison ◽  
Pcr Rflp

The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia

Parasitology ◽  
1998 ◽  
Vol 117 (1) ◽  
pp. 1-13 ◽  
Author(s):  
K. VICTOIR ◽  
A. L. BAÑULS ◽  
J. AREVALO ◽  
A. LLANOS-CUENTAS ◽  
R. HAMERS ◽  
...  
Keyword(s):  
Gene Locus ◽  
Genetic Characterization ◽  
Restriction Analysis ◽  
Pcr Amplification ◽  
Rflp Analysis ◽  
Restriction Enzymes ◽  
Pcr Rflp ◽  
Natural Isolates ◽  
Phenotype Diversity

In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a reference. On the one hand gp63–RFLP evidenced an extensive polymorphism and revealed specific markers for subgenus, species and geographical populations: congruence with MLEE was demonstrated statistically. The particular interest of gp63–RFLP was illustrated by infra-specific polymorphism, because of the possible relationship with phenotype diversity. On the other hand intra-genic amplification was less resolutive than gp63–RFLP, but also allowed discrimination of the 2 subgenera (PCR alone) and all the species tested in the subgenus Viannia (PCR–RFLP). PCR–RFLP presents an important operational advantage as it allows genetic characterization of minute amounts of parasites, using Leishmania specific primers. The polymorphism revealed by gp63–RFLP and PCR–RFLP illustrates the very high genomic and genetic plasticity of gp63 genes.

scholarly journals Genetic characterization of Toxoplasma gondii in Yunnan black goats (Capra hircus) in southwest China by PCR-RFLP

2015 ◽  
Vol 8 (1) ◽  
pp. 57 ◽  
Author(s):  
Qiang Miao ◽  
Si-Yang Huang ◽  
Si-Yuan Qin ◽  
Xin Yu ◽  
Yan Yang ◽  
...  
Keyword(s):  
Toxoplasma Gondii ◽  
Southwest China ◽  
Genetic Characterization ◽  
Capra Hircus ◽  
Pcr Rflp

Molecular genetic characterization of the cervid strain (‘northern form’) ofEchinococcus granulosus

Parasitology ◽  
1994 ◽  
Vol 109 (2) ◽  
pp. 215-221 ◽  
Author(s):  
J. Bowles ◽  
D. Blair ◽  
D. P. McManus
Keyword(s):  
Genetic Characterization ◽  
Molecular Genetic ◽  
Rflp Analysis ◽  
Domestic Sheep ◽  
Rflp Pattern ◽  
Its1 Region ◽  
Pcr Rflp ◽  
Mitochondrial Nd1 ◽  
Molecular Genetic Characterization

SUMMARYWe have used a number of molecular genetic approaches to characterize the cervid strain (‘northern form’) ofEchinococcus granulosus.PCR–RFLP analysis of the nuclear ITS1 region of the rDNA repeat can readily distinguish the cervid form from other strains ofE. granulosus. The complexity of the RFLP patterns obtained suggests that a number of distinct ITS1 types are present in this strain which may represent an inter-strainE. granulosushybrid. Mitochondrial CO1 sequence of the cervid genotype was ambiguous at 18 positions and closely resembles a cluster of previously characterizedE. granulosusgenotypes, G1 (common, domestic sheep)/G2 (Tasmanian sheep)/G3 (buffalo). In contrast, mitochondrial ND1 sequence, although unique, suggests that the cervid form is most similar to strains represented by the G6 (camel)/G7 (pig) genotypes. We assume that the CO1 and ND1 sequences obtained for the cervid genotype are linked in a single mitochondrial genome although this is difficult to explain if conventional molecular genetics of mitochondrial DNA are assumed. Based on its unique ND1 sequence and ITS1 PCR–RFLP pattern, the cervid strain appears to represent a distinct genotype (designated G8) ofE. granulosus.

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