Fine-tuning of a COI PCR-RFLP assay for fast genetic characterization of Spanish white-clawed crayfish

The Thyroglobulin (TG) and Leptin (LEP) genes are two candidate genes that widely used for molecular selection to improve carcass traits in beef cattle. This research was carried out to identify the genetic characterization of TG and LEP genes from 47 heads of Pasundan cows at West Java using PCR-RFLP method. Research shows that TG gene of Pasundan cattle is monomorphic with C allele as the dominant allele (1.00). However, LEP gene of Pasundan cattle is polymorphic with C allele as the dominant allele (0.98) and T as the rare allele (0.02). The polymorphic informative content (PIC) and numberof effective allele (ne) values in the LEP gene in the animal studied were 0.04 and 1.04 respectively. It was concluded that TG/BstYI and LEP/Sau3AI gene in the present study can not be used as molecular selection in Pasundan cattle. These results are important as the basic information for preparing the molecular selection program in the future.

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Application of kDNA Minicircle PCR-RFLP to Characterize Leishmania donovani Clinical Isolates Obtained from Post-Kala-Azar Dermal Leishmaniasis in Eastern Nepal

Canadian Journal of Infectious Diseases and Medical Microbiology ◽

10.1155/2019/9392414 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8

Author(s):

Ojesh Pokhrel ◽

Keshav Rai ◽

Narayan Raj Bhattarai ◽

Suman Rijal ◽

Arpana Rijal ◽

...

Keyword(s):

Leishmania Donovani ◽

Clinical Isolates ◽

Rdna Genes ◽

Kala Azar ◽

Pcr Rflp ◽

Potential Reservoir ◽

Rflp Assay ◽

Epidemiological Characterization

Post-kala-azar dermal leishmaniasis (PKDL) is a skin manifestation of visceral leishmaniasis (VL) which develops after apparent cure in some patients. PKDL is considered as the potential reservoir for the VL infection. Molecular epidemiological characterization of L. donovani isolates obtained from VL and PKDL isolates is essentially required in order to understand the transmission dynamics of the VL infection. To date, genetic variation among the VL and PKDL L. donovani isolates was not fully elucidated. Therefore, 14 clinical isolates from VL and 4 clinical isolates from PKDL were speciated by hsp70 and rDNA genes. Further characterization of L. donovani by haspB PCR demonstrates two different genotypes. All PKDL isolates have the same genetic structure. kDNA PCR-RFLP assay revealed 18 different genotypes; however, structural analysis showed the two distinct kDNA genotype population (k = 2). The kDNA fingerprint patterns of parasites from hilly districts were clustered separately from low-land districts. Therefore, further study with a large number of samples is urgently required for systematic characterization of the clinical isolates to track the molecular epidemiology of the Leishmania donovani causing VL and the role of PKDL as a reservoir.

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PCR-RFLP and sequence analysis of a non-ribosomal fragment for genetic characterization of European stone fruit yellows phytoplasmas infecting various Prunus species

Molecular and Cellular Probes ◽

10.1006/mcpr.2000.0304 ◽

2000 ◽

Vol 14 (3) ◽

pp. 171-179 ◽

Cited By ~ 9

Author(s):

W Jarausch ◽

C Saillard ◽

JM Broquaire ◽

M Garnier ◽

F Dosba

Keyword(s):

Sequence Analysis ◽

Genetic Characterization ◽

Stone Fruit ◽

Prunus Species ◽

Pcr Rflp

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USE OF PCR-RFLP FOR GENETIC CHARACTERIZATION OF ECHINOCOCCUS GRANULOSUSISOLATES FROM EGYPT

Ain Shams Medical Journal ◽

10.21608/asmj.2020.106418 ◽

2020 ◽

Vol 71 (1) ◽

pp. 249-257

Author(s):

Doaa Nassar ◽

Hala Elwakil ◽

Hayam Ezz Eldin ◽

Hanan Abou-Seri ◽

Khalifa Khalifa

Keyword(s):

Genetic Characterization ◽

Pcr Rflp

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Occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in slaughtered domestic rabbits in central China

Parasite ◽

10.1051/parasite/2019035 ◽

2019 ◽

Vol 26 ◽

pp. 36 ◽

Cited By ~ 1

Author(s):

Weifeng Qian ◽

Wenchao Yan ◽

Chaochao Lv ◽

Rongzhen Bai ◽

Tianqi Wang

Keyword(s):

Toxoplasma Gondii ◽

Genetic Characterization ◽

Neospora Caninum ◽

Central China ◽

Occurrence Rate ◽

Its1 Region ◽

Pcr Rflp ◽

Parasite Infections ◽

Domestic Rabbits

Currently, information on the occurrence and genetic characterization of Toxoplasma gondii and Neospora caninum in tissues of rabbits in China is lacking. In this study, brain and heart samples from 470 slaughtered domestic rabbits were collected in Henan Province, Central China. The occurrence rate of T. gondii and N. caninum DNA detected by nested PCR was 2.8% and 2.1%, respectively. There were no significant differences (p > 0.05) in the frequency of the two parasite infections in relation to sex, breed, and region. Three out of 13 T. gondii-positive samples were completely or partially genotyped at 11 genetic markers using PCR-RFLP, and one was identified as ToxoDB genotype #9. For N. caninum, three different sequences at the ITS1 region and two genotypes at the MS5 microsatellite locus were identified. To our knowledge, this is the first genetic characterization of N. caninum isolates from rabbits.

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Molecular genetic characterization of new bovine kappa-casein alleles CSN3F and CSN3G and genotyping by PCR-RFLP

Animal Genetics ◽

10.1111/j.1365-2052.1996.tb00976.x ◽

2009 ◽

Vol 27 (5) ◽

pp. 347-349 ◽

Cited By ~ 17

Author(s):

E-M Prinzenberg ◽

S Hiendleder ◽

T Ikonen ◽

G Erhardt ◽

E-M Prinzenberg ◽

...

Keyword(s):

Genetic Characterization ◽

Molecular Genetic ◽

Pcr Rflp ◽

Molecular Genetic Characterization

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Molecular Detection and Genetic Characterization of Toxoplasma gondii in Farmed Minks (Neovison vison) in Northern China by PCR-RFLP

PLoS ONE ◽

10.1371/journal.pone.0165308 ◽

2016 ◽

Vol 11 (11) ◽

pp. e0165308 ◽

Cited By ~ 10

Author(s):

Wen-Bin Zheng ◽

Xiao-Xuan Zhang ◽

Jian-Gang Ma ◽

Fa-Cai Li ◽

Quan Zhao ◽

...

Keyword(s):

Toxoplasma Gondii ◽

Molecular Detection ◽

Genetic Characterization ◽

Northern China ◽

Neovison Vison ◽

Pcr Rflp

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The gp63 gene locus, a target for genetic characterization of Leishmania belonging to subgenus Viannia

Parasitology ◽

10.1017/s0031182098002789 ◽

1998 ◽

Vol 117 (1) ◽

pp. 1-13 ◽

Cited By ~ 46

Author(s):

K. VICTOIR ◽

A. L. BAÑULS ◽

J. AREVALO ◽

A. LLANOS-CUENTAS ◽

R. HAMERS ◽

...

Keyword(s):

Gene Locus ◽

Genetic Characterization ◽

Restriction Analysis ◽

Pcr Amplification ◽

Rflp Analysis ◽

Restriction Enzymes ◽

Pcr Rflp ◽

Natural Isolates ◽

Phenotype Diversity

In the present study the gp63 gene locus was used as a target for genetic characterization of Leishmania parasites by 2 methods: (i) RFLP analysis with several restriction enzymes (gp63–RFLP), and (ii) intra-genic PCR amplification coupled with restriction analysis (PCR–RFLP). Both methods were applied to a large number of natural isolates belonging to 4 species of the subgenus Viannia, namely L. (V.) braziliensis, L. (V.) peruviana, L. (V.) guyanensis and L. (V.) lainsoni: reference stocks of subgenus Leishmania were included as outgroups. Multilocus isoenzyme typing (MLEE) was used as a reference. On the one hand gp63–RFLP evidenced an extensive polymorphism and revealed specific markers for subgenus, species and geographical populations: congruence with MLEE was demonstrated statistically. The particular interest of gp63–RFLP was illustrated by infra-specific polymorphism, because of the possible relationship with phenotype diversity. On the other hand intra-genic amplification was less resolutive than gp63–RFLP, but also allowed discrimination of the 2 subgenera (PCR alone) and all the species tested in the subgenus Viannia (PCR–RFLP). PCR–RFLP presents an important operational advantage as it allows genetic characterization of minute amounts of parasites, using Leishmania specific primers. The polymorphism revealed by gp63–RFLP and PCR–RFLP illustrates the very high genomic and genetic plasticity of gp63 genes.

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