scholarly journals Single Strand Conformation Polymorphism Method for Initial Detection DNA Sequences Homogeneity

2010 ◽  
Vol 17 (1) ◽  
pp. 50-52
Author(s):  
TOPIK HIDAYAT ◽  
ADI PANCORO
Keyword(s):  
Dna Sequences ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Conformation Polymorphism

scholarly journals Molecular Differentiation of Four Species of Oropsylla (Siphonaptera: Ceratophyllidae) Using PCR-Based Single Strand Conformation Polymorphism Analyses and DNA Sequencing

2020 ◽  
Author(s):  
Jessica T Thoroughgood ◽  
James S Armstrong ◽  
Brandon White ◽  
Clare A Anstead ◽  
Terry D Galloway ◽  
...  
Keyword(s):  
Dna Sequencing ◽  
Dna Sequences ◽  
Related Species ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Morphological Identification ◽  
Rrna Gene ◽  
28S Rrna ◽  
Closely Related Species ◽  
Conformation Polymorphism

Abstract It is often difficult to distinguish morphologically between closely related species of fleas (Siphonaptera). Morphological identification of fleas often requires microscopic examination of internal structures in specimens cleared using caustic solutions. This process degrades DNA and/or inhibits DNA extraction from specimens, which limits molecular-based studies on individual fleas and their microbiomes. Our objective was to distinguish between Oropsylla rupestris (Jordan), Oropsylla tuberculata (Baker), Oropsylla bruneri (Baker), and Oropsylla labis (Jordan & Rothschild) (Ceratophyllidae) using PCR-based single strand conformation polymorphism (SSCP) analyses and DNA sequencing. A 446 bp region of the nuclear 28S ribosomal RNA (rRNA) gene was used as the genetic marker. The results obtained for 36 reference specimens (i.e., fleas that were morphologically identified to species) revealed no intraspecific variation in DNA sequence, whereas the DNA sequences of the four species of Oropsylla differed from one another at two to six nucleotide positions. Each flea species also had a unique SSCP banding pattern. SSCP analyses were then used to identify another 84 fleas that had not been identified morphologically. DNA sequencing data confirmed the species identity of fleas subjected to SSCP. This demonstrates that PCR-SSCP combined with DNA sequencing of the 28S rRNA gene is a very effective approach for the delineation of four closely related species of flea.

Screening for mutations in factor VIII gene using the single-strand conformation polymorphism

1995 ◽  
Vol 5 (4) ◽  
pp. 357-359
Author(s):  
Khédoudja Nafa ◽  
Farida Meriane ◽  
Thouraya Chellali ◽  
Mohamed Benabadji ◽  
Abderrezak Reghis ◽  
...  
Keyword(s):  
Factor Viii ◽  
Single Strand Conformation Polymorphism ◽  
Single Strand ◽  
Factor Viii Gene ◽  
Conformation Polymorphism
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