scholarly journals Prevalence of occult hepatitis B virus infection among blood donors in Ouagadougou, Burkina Faso

2021 ◽  
Vol 22 (3) ◽  
pp. 359-364
Author(s):  
A. Ky/Ba ◽  
M. Sanou ◽  
A.S. Ouédraogo ◽  
I.B. Sourabié ◽  
A.Y. Ky ◽  
...  
Keyword(s):  
Hepatitis B ◽  
Burkina Faso ◽  
Real Time ◽  
Real Time Pcr ◽  
Blood Donors ◽  
Hbv Dna ◽  
Rt Pcr ◽  
Occult Hepatitis ◽  
B Virus ◽  
Polymerase Chain

Background: In Burkina Faso, the polymerase chain reaction (PCR) assay is not routinely used in the biological qualification of blood donations and this constitutes a risk factor for the transmission of occult hepatitis B virus (HBV) infection during blood transfusion. The objective of this study is to determine the prevalence of occult B infection (OBI) among blood donors for the purposes of improved blood safety in Burkina Faso. Methodology: A descriptive cross-sectional study of 300 HBsAg negative blood donors was conducted in the city of Ouagadougou, Burkina Faso from April to October 2020. Anti-HBc antibody was determined using the BOSON® brand rapid tests. HBV DNA was detected in 75 selected donors by real-time PCR (rt PCR) using the 7500 Fast Real Time PCR assay technique. Results: Of the 300 HBsAg negative donors, 208 (69.3%) were males while 92 (30.7%) were females, with average age of 30.18 years. Anti-HBc antibody was detected in 39 cases (13%). Of the 75 donor samples tested by rt PCR, 3 (4%) were positive for HBV DNA (occult B infection); 2 of which were anti-HBc antibody positive (seropositive OBI) while 1 was anti-HBc antibody negative (seronegative OBI). Conclusion: Given the prevalence of OBI of 4% in this study and its consequences in blood recipients, it appears necessary that in addition to the classic serological markers of hepatitis B, to test for the presence of HBV DNA among blood donors in order to improve transfusion safety. Keywords: Prevalence, Occult B infection; Blood donors, Ouagadougou.   French title: Prévalence de l'infection occulte par le virus de l'hépatite B chez les donneurs de sang à Ouagadougou, Burkina Faso Contexte: Au Burkina Faso, la polymérase chain réaction (PCR) n’est pas utilisée lors de la qualification biologique des dons et cela constitue un facteur de risque de transmission de l’Infection Occulte du virus B (VHB) lors des transfusions sanguines. L’objectif de cette étude était de déterminer la prévalence de l’infection occulte B chez les donneurs de sang en vue d’une meilleure sécurité transfusionnelle. Méthodologie: Une étude transversale prospective, réalisée d’avril à octobre 2020 dans la ville de Ouagadougou incluant 300 donneurs de sang AgHBs négatif. L’anticorps anti HBc a été déterminé par les tests rapides de marque BOSON®. L’ADN du VHB a été recherché chez 75 donneurs par PCR en temps réel (rt PCR) avec le 7500 Fast Real Time PCR. Résultats: Parmi les 300 donneurs AgHBs négatifs, 208 (69,3%) étaient des hommes et 92 (30,7%) des femmes. L’âge moyen était de 30,18 ans. La recherche de l’Ac anti-HBc était positive dans 39 cas (13 %). Parmi les 75 échantillons passés à la rt PCR, 3 (4%) étaient positifs pour l’ADN du VHB. Sur les 3 cas d’ADN VHB positifs, 2 (66,67%) étaient positifs Ac anti HBc et 1 (33,33%) Ac anti HBc négatif. Conclusion: Compte tenu de la prévalence de l’infection occulte B et ses conséquences chez les donneurs de sang et chez les receveurs, il devient nécessaire de rechercher, en plus des marqueurs sérologiques classiques de l’hépatite B, l’ADN VHB pour une meilleure sécurité transfusionnelle. Mots clés: Prévalence, Infection occulte de l’HBV, Donneurs de sang, Ouagadougou  

scholarly journals Hepatitis B virus DNA in Blood Donors Positive of Anti-Hepatitis B Core Antibodies and Negative for Surface Antigen in Hawler Major Blood Bank, Kurdistan Region, Iraq

2018 ◽  
Vol 60 (1) ◽  
pp. 57-61
Author(s):  
Rasha N. Hassan ◽  
Ali H. Hussain
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Blood Donors ◽  
Hbv Dna ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Hbs Ag

Background: Occult Hepatitis B virus infection (OBI) among blood donors is an important medical concern.Objectives: This study was done to detect the presence of occult hepatitis B virus infections among blood donors with negative hepatitis B surface antigen and positive total anti-hepatitis B core antibodies in Hawler Major Blood Bank in Hawler city/Kurdistan Region of Iraq.Methods: A total number of 12,185 blood donors in Hawler Major Blood Bank were screened for HBsAg and total anti-HBcAb using ELISA technique, and then positive results were retested by confirmatory technique by Chemiluminescence assay. All HBsAg-/HBcAb+ were selected as the study group; HBV DNA was tested among HBsAg-/HBcAb+ by conventional PCR and Real time-PCR. Clinical and demographic data of study group were recorded.Results: Among the 12,185 blood donors, HBsAg was positive in 27 (0.22%) donors using Chemiluminescence assay, the frequency of HBs Ag -/ HBc Ab+ was 276 (2.27%), and then the total prevalence of HBV infection in all blood donors was 2.49%. Among the 276 HBs Ag-/HBcAb+, occult hepatitis B virus infection (OBI) was positive in 39.1% (108/276) using conventional PCR and Real time-PCR techniques, while the prevalence among all blood donors (n=12,185) was 0.09%. Testing of HBV-DNA in HBs Ag -/ HBc Ab+ group for OBI was done by qualitative PCR (positive HBV-DNA=102/276) or by quantitative Real time-PCR (positive HBV-DNA=108/276).Conclusions: The OBI is frequently detected among blood donors in Hawler city especially those have HBsAg-/HBcAb+, and the total anti-HBcAb is an essential serological marker for screening HBV among blood donors. The risk factors for developing OBI among blood donors should be elucidated.   الحامض النووي  الريبوزي منقوص الأكسجين للفايروس الكبدي نوع ب في دم المتبرعين والموجب لأضداد اللب للفايروس وسالب للستضد السطحي للفايروس في مصرف الدم الرئيسي في اربيل/كردستان العراق  رشا نزار حسن علي حاتم حسين نظرة اولية: ان تواجد الاصابة  بالفايروس الكبدي نوع ب لدى المتبرعين بالدم يعتبر من الحالات الطبية التي يجب الانتباه اليها. الاهداف: تم اجراء هذه الدراسة على المتبرعين بالدم في مصرف الدم الرئيسي في مدينة اربيل/كردستان العراق , لغرض التحري عن وجود الاصابات الكبدية الفايروسية نوع ب المخفية لدى المتبرعين بالدم والذين يكون المستضاد السطحي الفايروسي الكبدي نوع ب (HBsAg) سالبا لديهم ويكون المضاد اللبي الكلي لنفس الفايروس(anti-HBcAb) موجبا. طريقة البحث: تم فحص اثنا عشر الفا ومائه وخمس وثمانين متبرع بالدم في مصرف الدم الرئيسي في مدينة اربيل لغرض الكشف عن (HBsAg) و  (anti-HBcAb) وان النتائج الموجبة تم اعادة فحصها بطرق فحص تأكيدية. ان كل المتبرعين ذوو النتائج (السالبة HBsAg ) / و (الموجبة anti-HBcAb) تم انتقئهم كمجموعة الدراسة وتم فحص وجود الحامض النووي  الريبوزي منقوص الأكسجين للفايروس الكبدي نوع ب لديهم في الدم بطريقتين: طريقة تفاعل البوليميراز المتسلسل التقليدية وطريقة تفاعل البوليميراز المتسلسل اللحظي. كما تم تسجيل البيانات الديموغرافية والسريرية لمجموعة الدراسة. النتائج:  لدى فحص المتبرعين البالغ عددهم  اثنا عشر الفا ومائه وخمس وثمانين متبرع تبين وجود سبعة وعشرون اصابة (0.02%)  بالمستضاد (HBsAg) اما عدد المتبرعين الذين ليس لديهم  / HBsAg والموجبة للمضاد anti-HBcAb  فقد كان 276 (2.27%) وقد تبين ان نسبة انتشار الخمج البدي الفايروسي نوع ب لدى المتبرعين كان 2.49%. عند فحص مجموعة الدراسة البالغ عددها 276 والذين كانت نتيجة فحصهم موجبة للمضاد /HBcAb,وسالبة للمستضاد HBsAg ,تبين وجود الخمج الفايروسي الكبدي نوع ب المخفي في 39.1% (108/276),  بينما كانت نسبة انتشار نفس الخمج لدى مجموع المتبرعين الكلي البالغ 12185 هو 0.09%. لقد كانت نتيجة فحص وجود الحامض النووي  الريبوزي منقوص الأكسجين للفايروس الكبدي نوع ب بالطريقة القليدية هي 102 متبرع من مجموعة الدراسة الباغة 276 اما النتيجة لنفس مجموعة الدراسة لنفس الحامض النووي بطريقة تفاعل البوليميراز المتسلسل اللحظي كانت 108. الاستنتاج: لقد بينت هذه الدراسة وجود وجود الخمج الفايروسي الكبدي نوع ب المخفي بصورة متكررة لدى المتبرعين بالدم في مدينة اربيل وخاصة الذين تكون حالتهم  موجبة للمضاد /HBcAb, وسالبة للمستضاد HBsAg كما بينت الدراسة ان فحص  المضاد اللبي للفايروس الكبدي نوع ب هو ضروري عند التحري عن وجود وجود الخمج الفايروسي الكبدي نوع ب لدى المتبرعين بالدم, كما انه من الضروري التقصي عن عوامل الخطورة التي تؤدي الى الاصابة بالخمج الفايروسي الكبدي نوع ب المخفي. الكلمات المفتاحية: المتبرعون بالدم, الخمج الفايروسي الكبدي نوع ب المخفي, مدينة اربيل, مصرف الدم, تفاعل البوليمراز المتسلسل التقليدي واللحظي

scholarly journals Serological and Molecular Detection of Hepatitis B virus among patients referred to Kurdistan Center for Hepatology and Gastroenterology in Sulaimani City/Kurdistan Region of Iraq

2020 ◽  
Vol 4 (2) ◽  
pp. 117
Author(s):  
Raz Sirwan Abdulla ◽  
Salih Ahmed Hama
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Hbv Dna ◽  
Molecular Techniques ◽  
Enzyme Linked Immunosorbent Assay ◽  
Rt Pcr ◽  
B Virus ◽  
Polymerase Chain ◽  
Pcr Techniques ◽  
Positive Results

Hepatitis B virus infection is caused by the hepatitis B virus, a major global health problem. This infection can lead to chronic conditions, followed by cirrhosis and hepatocellular carcinoma (HCC). The current study was aimed to detect HBV using serological and molecular techniques. During 2019, 300 blood samples were collected from Kurdistan Center for Hepatology and Gastroenterology in Sulaimani city. Enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR) techniques were used for the detection of HBsAg and HBV DNA, respectively. Obtained results were revealed that 92 out of 300 tested patients (30.66%) seropositive for HBsAg. Among 92 seropositive patients, 53 were shown positive results for HBV DNA by RT-PCR. Dental clinic visiting and dialysis were among the important risk factors for HBV transmission. The vast majority of positive results were among males. Smokers showed relatively high rates of positive results. One-third of the referred patients who had liver complaints were positive for HBsAg. More than half of the seropositive patients showed RT-PCR positive results. It was concluded that the molecular method (RT-PCR) is more sensitive and gives a more accurate result than serology (ELISA). Therefore, it can be used as a diagnostic tool for HBV detection.

scholarly journals Real Time PCR HBV-DNA Analysis in HBsAg Positive Patients

2015 ◽  
Vol 10 (2) ◽  
pp. 95-99
Author(s):  
Wasila Rahman ◽  
Muhammad Rabiul Hossain ◽  
Arif Ahmed Khan ◽  
Debashish Saha ◽  
SM Mahbubul Alam ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Antiviral Therapy ◽  
Real Time ◽  
Real Time Pcr ◽  
Armed Forces ◽  
Hbv Dna ◽  
Health Concern ◽  
B Virus ◽  
Pcr Method

Introduction: The hepatitis B virus is a global public health concern and leading cause of chronic liver disease in Bangladesh. For the diagnosis and monitoring of treatment of Hepatitis B virus infection, HBV-DNA detection and quantification is now extensively used worldwide.Objectives: The objective of this study was to detect HBV-DNA by real time PCR method in HBsAg positive patients, to compare the results of HBVDNA detection with HBeAg and Anti-HBe and to monitor the response after antiviral therapy in chronic hepatitis B patients and also to observe the intensity of hepatitis B infection in relation to age and sex.Methods: This was a cross sectional type of study conducted in Armed Forces Institute of Pathology (AFIP), Dhaka Cantonment. In this study, 56 sera of HBsAg positive patients were selected who all were subjected to do HBV-DNA (real time PCR) analysis during the period of 29 July to 30 0ctober, 2013.Results: Out of 56 HBsAg positive patients, HBV-DNA was detected in 34 patients. Among these, 8 (23.5%) patients were HBeAg positive, 16 (47%) patients were anti-HBe positive and 10 (29.5%) were negative for both HBeAg and anti-HBe. Age limit of patients was up to 60 years. HBV-DNA positive patients showed male predominance; 26 (76.5%) patients were male and 8 (23.5%) patients were female. Mean age of the patients was 35±14 years. Among 56 HBsAg positive patients, fifteen were receiving antiviral therapy. Out of them, HBV-DNA was decreased among 4 patients and could not be detected among 11 patients.Conclusion: Real time PCR method of detection of HBV-DNA is very important in patients who are HBeAg negative and this method is also applied to monitor treatment response to antivirals and to detect occult HBV infections immune control phase and also to detect reactivation of HBV cases.Journal of Armed Forces Medical College Bangladesh Vol.10(2) 2014

Prevalence of Occult Hepatitis B Infection among HBsAg Negative Blood Donors in Golestan Province

2019 ◽  
Author(s):  
Rana Tabar Asad Laleh ◽  
Zohreh Sharifi ◽  
Akbar Pourfathollah ◽  
Shahram Samei
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Blood Donors ◽  
Hbv Dna ◽  
High Rate ◽  
Analytical Sensitivity ◽  
Occult Hepatitis ◽  
B Virus ◽  
Occult Hepatitis B ◽  
Golestan Province

Background and Aims: Occult hepatitis B virus infection (OBI) is known as an important source of hepatitis B virus (HBV) infection. It is categorized as Hepatitis B surface antigen (HBsAg) not being present and low DNA viral load in serum. In this study, an attempt was made to investigate the outbreak of anti-HBc and OBI among the HBsAg-negative donors in Golestan province. Materials and Methods: The present cross-sectional experiment was conducted on 3500 voluntary blood donors in Golestan province to examine the presence of human immunodeficiency viruses Ag-Ab, HBsAg, and hepatitis C virus Ab. Then, samples with negative results for the mentioned tests were screened for total HBc antibody (IgM-IgG) through ELISA technique. Afterward, HBV-DNA extraction and R-T PCR assay were conducted for all HBsAg negative samples by using Real ART HBV LC PCR kit on a Light Cycler instrument. Results: The study participants included 3255 (93%) male and 245 (7%) female. In general, 385 (11%) out of 3500 samples were anti-HBc positive. HBV-DNA results for every sample with either positive or negative anti-HBc were found to be negative. Conclusions: As the area under study has a high rate of anti-HBc outbreak (11%) without the presence of HBV-DNA, anti-HBc screening can cause blood donor deferrals and limit blood supply; therefore, the HBsAg test with high analytical sensitivity is recommended for HBV screening in this area. Regarding the cost analyses and also the status of HBV endemicity, HBsAg test along with ID-NAT is preferable, if possible, for improving blood safety.

Signature of chronic hepatitis B virus infection in nails and hair

2020 ◽  
Author(s):  
Haruki Komatsu ◽  
Ayano Inui ◽  
Enkhtaivan Odmaa ◽  
Yoshinori Ito ◽  
Shuichiro Umetsu ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Hbv Dna ◽  
Chimeric Mice ◽  
Hair Samples ◽  
B Virus ◽  
Chronic Hbv ◽  
Immunochemical Staining

Abstract Background: Hepatitis B virus (HBV) is detected in extrahepatic tissues of individuals with HBV infection. Whether nails and hair contain HBV has been unknown. Methods: We examined two patient groups: those with chronic HBV infection alone (n=71), and those with both chronic HBV and hepatitis delta virus (HDV) infections (n=15). HBV DNA in the patients' fingernails and hair were measured by real-time PCR. Hepatitis B surface antigen (HBsAg) of fingernails was evaluated by an enzyme immunoassay. HDV RNA in fingernails was measured by real-time PCR. Immunochemical staining was performed on nails. We used chimeric mice with humanized livers to evaluate the infectivity of nails.Results: Of the 71 pairs of HBV-alone nail and hair samples, 70 (99%) nail and 60 (85%) hair samples were positive for β-actin DNA. Of those 70 nail samples, 65 (93%) were HBV DNA-positive. Of the 60 hair samples, 49 (82%) were HBV DNA-positive. The serum HBV DNA level of the nail HBV DNA-positive patients was significantly higher than that of the nail HBV DNA-negative patients. The hair HBV DNA-positive patients' serum HBV DNA level was significantly higher compared to the hair HBV DNA-negative patients. The nail HBV DNA level was significantly higher than the hair HBV DNA level. The nails and hair HBV DNA levels were correlated (r=0.325, p<0.05). A phylogenetic tree analysis of the complete genome sequence of HBV isolated from nails and hair identified the infection source. Of the 64 nail samples, 38 (59%) were HBsAg-positive. All 15 pairs of chronic HBV/HDV infection nail and hair samples were β-actin DNA-positive. However, nail HBV DNA was detected in two patients (13%). None of the 15 patients were positive for hair HBV DNA. Nail HDV RNA was detected in three patients (20%). Of the 15 patients, eight (53%) were nail HBsAg-positive. HBsAg and hepatitis delta (HD) antigen were detected in the nails by immunochemical staining. Chimeric mice were not infected with PBS containing HBsAg and HBV DNA elucidated from nails.Conclusions: Nails and hair were the reservoir of HBV DNA. Moreover, nails can contain HBsAg, HDV RNA, and HD antigen.

scholarly journals COBAS AmpliPrep-COBAS TaqMan Hepatitis B Virus (HBV) Test: a Novel Automated Real-Time PCR Assay for Quantification of HBV DNA in Plasma

2007 ◽  
Vol 45 (3) ◽  
pp. 828-834 ◽  
Author(s):  
T. Allice ◽  
F. Cerutti ◽  
F. Pittaluga ◽  
S. Varetto ◽  
S. Gabella ◽  
...  
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Hbv Dna ◽  
Pcr Assay ◽  
Cobas Taqman ◽  
B Virus

scholarly journals Comparison of a novel real-time PCR method (RTA) and Artus RG for the quantification of HBV DNA and HCV RNA

2017 ◽  
Vol 11 (07) ◽  
pp. 543-548
Author(s):  
Sibel Aydogan ◽  
Ziya Cibal Acikgoz ◽  
Aysegul Gozalan ◽  
Fisun Kirca ◽  
Tuba Muderris ◽  
...  
Keyword(s):  
Quality Control ◽  
Real Time ◽  
Hbv Dna ◽  
Clinical Samples ◽  
Hcv Rna ◽  
Rt Pcr ◽  
B Virus ◽  
Pcr Method ◽  
Polymerase Chain

Introduction: The purpose of this study was to evaluate and compare two manual isolation and real-time (RT) polymerase chain reaction (PCR) kits (RTA RT-PCR with RTA isolation kit and Artus RG RT-PCR with QIAamp isolation kit) for molecular diagnosis of hepatitis B virus (HBV) and hepatitis C virus (HCV) infections. Methodology: The study was conducted on 121 and 54 clinical samples for the detection of HBV DNA and HCV RNA, respectively, with an additional 8 HCV RNA external quality control samples. Results: Though a high correlation was observed between the two kits for the HBV DNA (r = 0.955, p = 0.001) and HCV RNA quantifications (r = 0.828, p = 0.001), discordant results were found in nine of the HBV DNA and in six of the HCV RNA samples. The mean difference between the two systems was found to be 0.4 log IU/mL in Quality Control for Molecular Diagnostics (QCMD) HCV RNA samples by Bland-Altman analysis. Conclusions: Although there was a high correlation between HBV DNA and HCV RNA tests according to the results of the study, the RTA system requires improvement for the determination of HCV RNA.

scholarly journals DETERMINATION OF VARIOUS CLINICAL STAGES OF CHRONIC HEPATITIS B THROUGH MEASURING VIRUS LOAD DNA BY REAL-TIME POLYMERASE CHAIN REACTION (RT-PCR)

2020 ◽  
pp. 1-2
Author(s):  
Subhash Kumar Saw ◽  
MD. Mohammad Sohail ◽  
Jainendra Kumar
Keyword(s):  
Polymerase Chain Reaction ◽  
Chronic Hepatitis ◽  
Viral Load ◽  
Hepatitis B ◽  
Real Time ◽  
Chronic Hepatitis B ◽  
Hbv Dna ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

Objective and Aim: There was a paradigm shift of hepatitis B (CHB) diagnosis as clinicians are shifted to molecular diagnostic methods from serological one. Specially in molecular system to determine response of treatment as well as different stages of infection as well as recovery by quantification of viral DNA load through real time polymerase chain reaction (RT-PCR). The main objective of the study is to determine the various clinical stages of chronic hepatitis B through measuring virus load DNA by real-time polymerase chain reaction (RT-PCR) Material and Methods: This is a retrospective study of those patients whose ALT (elevated) and HBeAg (positive) status is known. Serum fraction were initially obtained after 4 hour centrifugation of blood sample and nucleic acid was extracted at -80 °C. Qiagen DNA extraction kit were used to extract DNA. 48-well MiniOpticon by Bio-red machine and with the help of Geno-sense HBV quantitative PCR kit, real-time polymerase chain reaction (RT-PCR) was conducted. Result: The study was conducted in 64 patients. It has been found that among this patients inactive carriers that is ALT normal and HBeAg-negative were 27 (42.2%) and rest of the patients had HBeAg-positive or HBeAg-negative with ALT elevated that is they were chronic active hepatitis B patients. HBeAg was negative in 42 (65.6%) and positive in 22 (33.4%) subjects. 15 (23%) patients were infected with Chronic hepatitis B among the patients who were HBeAg-negative. Among 64 subjects, detectable viral load was found in 55 (86%) CHB patients. A significantly lower (median 5.6 × 105) serum HBV DNA load were found in HBeAg-negative16 patients as compare to 26 patients with higher viral load (median 2.5 × 108) and were HBeAg-positive. It has also found that viral load was quite higher (median 1.5 × 103) in 27 inactive carriers. Antiviral therapy was started in HBeAg-negative 6 patients and HBeAg-positive 13 patients based on the viral load. Conclusion: Stages of CHB can be determined by Quantitation of HBV DNA based on ALT (elevated or not) and HBeAg (positive or negative) status. For those patients who are inactive carriers and HBeAg-negative with respect to viral load it could play an important role in assessment and to decide on antiviral therapy.

scholarly journals Detection of Hepatitis B Virus DNA in Chronic HBV Carriers and Correlation with HBeAg

2017 ◽  
Vol 15 (2) ◽  
pp. 33-36 ◽  
Author(s):  
Shamima Akther ◽  
Md Anwar Husain ◽  
HS Mubarak Hossain
Keyword(s):  
Hepatitis B Virus ◽  
Hepatitis B ◽  
Real Time ◽  
Real Time Pcr ◽  
Chronic Infection ◽  
Hbv Dna ◽  
B Virus ◽  
Study Population ◽  
Chronic Hbv ◽  
The Mean

Background: Chronic infection with hepatitis B virus causes a spectrum of diseases ranging from asymptomatic infective state to cirrhosis and hepatocellular carcinoma. The detection and quantification of HBV DNA plays an important role in diagnosing HBV infection as well as monitoring therapeutic responses.Methods: The aim of this study was to detect and quantited HBV DNA by real time PCR and to find out correlation HBeAg with HBV DNA in chronic carriers: The present study carried out among 61 previously diagnosed chronic hepatitis B patients.Results: The mean age of study population was 29.20 years. Among the 61 cases, 13(21.3%) were HBeAg positive and 48(78.7%) were HBeAg negative. By real time PCR, DNA detected in 45(73.8%) patients and 16(26.2%) patients were undetected. Association of HBeAg and HBV DNA was observed in 13 HBeAg positive cases, where 12(92.3%) had detectable DNA and in 48 HBeAg negative patients, 33(68.8%) had detectable DNA while 15(31.2%) were undetected. The present study observed a higher viral load (105copies/ml) among HBeAg positive patients (84.6%) than HBeAg negative patients (12.5%).Conclusion: The present study observed that, there was positive correlation among HBeAg and HBV DNA in chronic HBV carriers. However, there was some discordance observed among HBeAg and HBV DNA. Therefore, in addition to HBeAg, HBV DNA should be assessed for appropriate evaluation of CHB carriers.Chatt Maa Shi Hosp Med Coll J; Vol.15 (2); Jul 2016; Page 33-36

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