Objectives: To determine the prevalence of Acinetobacter spp. from different clinical specimens and detect different types of β-lactamase enzymes.
Methods: Different clinical samples were collected and 125 Acinetobacter spp. were isolated. Various biochemical tests were carried out to speciate the Acinetobacter spp. The antibiotic susceptibility pattern and β-lactamase enzymes like Extended spectrum β-lactamase (ESBL), Metallo β-lactamase (MBL) and AmpC β-lactamase were determined.
Results: Of the total 125 isolates, the most predominant species was Acinetobacter calcoaceticus-A. baumannii (Acb) complex (80%). Highest rate of isolation of Acinetobacter species were from in-patients (neonates’ blood sample). Among all, 44.8% isolates were found to be MDR with the majority being resistant to aminoglycosides, carbapenems and fluoroquinolones but not to colistin. ESBL, MBL and AmpC beta-lactamase was detected in 43.2%, 15.2% and 1.6% of the isolates respectively.
Conclusion: Acinetobacter calcoaceticus-A. baumannii complex should be considered for detection in hospitalized patients. The analysis of antibiotic susceptibility pattern and β-lactamases would be helpful to establish network surveillance in order to maintain and control the spread of these resistant strains.
Prevalence and antibiotic susceptibility pattern of ESBL producing Klebsiellae isolated from clinical specimens in a Nigerian tertiary hospital
