Comparative studies on the use of seller\'s, fluorescent staining techniques and animal inoculations in rabies diagnosis

2008 ◽  
Vol 3 (2) ◽  
Author(s):  
A A Chukwuedo ◽  
A O Olabode
Keyword(s):  
Comparative Studies ◽  
Fluorescent Staining ◽  
Staining Techniques ◽  
Rabies Diagnosis

scholarly journals Comparative description and ossification patterns of Dendropsophus labialis (Peters, 1863) and Scinax ruber (Laurenti, 1758)(Anura: Hylidae)

2017 ◽  
Author(s):  
Angélica Arenas Rodríguez ◽  
Juan Francisco Rubiano ◽  
Julio Mario Hoyos
Keyword(s):  
Comparative Studies ◽  
Skeletal Development ◽  
Spinal Column ◽  
Transverse Process ◽  
Life Cycles ◽  
Larval Stages ◽  
Staining Techniques ◽  
Ossification Sequences ◽  
Comparative Description ◽  
Ossification Sequence

Although comparative studies of anuran ontogeny have provided new data on heterochrony in the life cycles of frogs, most of them have not included Colombian species. Using different staining techniques, we describe the cranial and poscranial elements development in two hylid species, Scinax ruber and Dendropsophus labialis, providing new data for more comprehensive ontogenetic studies in Neotropical frogs. We examined specimens from Gosner stages 25 to 45. We found differences in the infrarostral and suprarostral cartilages, optic foramen, planum ethmoidale, and the gill apparatus. In the ossification sequence, one of the first elements to ossify were the transverse process of spinal column and atlas in both species, and the parasphenoid in the skull. New descriptions of skeletal development and ossification sequences of larval stages of these two species, especially data concerning the postcranium, contribute with useful information for analysis of sequential heterochrony, because although the hylids are widely known, there are few works (15 of 700 species) about ossification sequence that include the whole skeleton.

scholarly journals Rapid Detection of Specific Microbial Cells by Fluorescent Staining Techniques.

Eisei kagaku ◽  
1997 ◽  
Vol 43 (3) ◽  
pp. 145-154 ◽  
Author(s):  
Katsuji TANI ◽  
Nobuyasu YAMAGUCHI ◽  
Masao NASU
Keyword(s):  
Rapid Detection ◽  
Fluorescent Staining ◽  
Microbial Cells ◽  
Staining Techniques

scholarly journals COMPARISON OF ZIEHL-NEELSEN, KINYOUN’S AND FLUORESCENT STAINING FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SAMPLES BEFORE AND AFTER PETROFF’S CONCENTRATION TECHNIQUE

2018 ◽  
Vol 11 (4) ◽  
pp. 110
Author(s):  
Neelu Sree P ◽  
Terin J ◽  
Himadri Dutta ◽  
Kalyani M
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sputum Sample ◽  
Diagnostic Value ◽  
Fluorescent Staining ◽  
Concentration Technique ◽  
Before And After ◽  
Auramine O ◽  
Staining Methods ◽  
Staining Techniques ◽  
Microscopy Examination

 Objectives: The objectives of the study were to find out the presence of Mycobacterium tuberculosis in sputum samples using Ziehl-Neelsen (ZN), Kinyoun’s, and (auramine) fluorescent staining and to compare the three staining techniques with and without Petroff’s concentration and to find out the most preferable staining of M. tuberculosis. Methods: Sputum sample was collected and concentrated by 4% NaOH (Petroff’s concentration). Microscopic examination of the sample was done before concentration and after concentration by ZN staining, Kinyoun’s staining, and fluorescent staining (Auramine). Grading of acid-fast bacilli (AFB) by three staining was done before and after concentration according to RNTCP guidelines.Results: Total of 452 sputum samples were collected and subjected to microscopy examination by ZN, Kinyoun’s, and fluorescent staining methods to compare the presence or absence of AFB with or without concentration. Among the 452 sputum samples, total of 67 (15.6%) sputum samples were positive for the presence of AFB. Majority 40.3% (n=21) of TB positive patients were observed in the age group 51–60 years. The results of Auramine-O staining showed positive diagnoses in 15.9% of the samples; sensitivity was 100% and specificity 95.6%.Conclusion: The use of fluorescent staining significantly increases the diagnostic value of the smear, particularly where there are low-density bacilli which may escape detection on ZN stained smears.

scholarly journals Comparative description and ossification patterns of Dendropsophus labialis (Peters, 1863) and Scinax ruber (Laurenti, 1758)(Anura: Hylidae)

2017 ◽  
Author(s):  
Angélica Arenas Rodríguez ◽  
Juan Francisco Rubiano ◽  
Julio Mario Hoyos
Keyword(s):  
Comparative Studies ◽  
Skeletal Development ◽  
Spinal Column ◽  
Transverse Process ◽  
Life Cycles ◽  
Larval Stages ◽  
Staining Techniques ◽  
Ossification Sequences ◽  
Comparative Description ◽  
Ossification Sequence

Although comparative studies of anuran ontogeny have provided new data on heterochrony in the life cycles of frogs, most of them have not included Colombian species. Using different staining techniques, we describe the cranial and poscranial elements development in two hylid species, Scinax ruber and Dendropsophus labialis, providing new data for more comprehensive ontogenetic studies in Neotropical frogs. We examined specimens from Gosner stages 25 to 45. We found differences in the infrarostral and suprarostral cartilages, optic foramen, planum ethmoidale, and the gill apparatus. In the ossification sequence, one of the first elements to ossify were the transverse process of spinal column and atlas in both species, and the parasphenoid in the skull. New descriptions of skeletal development and ossification sequences of larval stages of these two species, especially data concerning the postcranium, contribute with useful information for analysis of sequential heterochrony, because although the hylids are widely known, there are few works (15 of 700 species) about ossification sequence that include the whole skeleton.

scholarly journals Comparative description and ossification patterns ofDendropsophus labialis(Peters, 1863) andScinax ruber(Laurenti, 1758) (Anura: Hylidae)

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4525 ◽  
Author(s):  
Angélica Arenas-Rodríguez ◽  
Juan Francisco Rubiano Vargas ◽  
Julio Mario Hoyos
Keyword(s):  
Comparative Studies ◽  
Vertebral Column ◽  
Skeletal Development ◽  
Life Cycles ◽  
Differential Staining ◽  
Large Group ◽  
Staining Techniques ◽  
Ossification Sequences ◽  
Comparative Description ◽  
Ossification Sequence

Although comparative studies of anuran ontogeny have provided new data on heterochrony in the life cycles of frogs, most of them have not included ossification sequences. Using differential staining techniques, we observe and describe differences and similarities of cranial and postcranial development in two hylid species,Scinax ruber(Scinaxinae) andDendropsophus labialis(Hylinae), providing new data of ontogenetic studies in these Colombian species. We examined tadpoles raining from Gosner Stages 25 to 45. We found differences between species in the infrarostral and suprarostral cartilages, optic foramen, planum ethmoidale, and gill apparatus. In both species, the first elements to ossify were the atlas and transverse processes of the vertebral column and the parasphenoid. Both species exhibited suprascapular processes as described in other hylids. Although the hylids comprise a large group (over 700 species), postcranial ossification sequence is only known for 15 species. Therefore, the descriptions of the skeletal development and ossification sequences provided herein will be useful for future analyses of heterochrony in the group.

Two simplified fluorescent staining techniques to observe infection structures of the oomycete Plasmopara viticola in grapevine leaf tissues

Micron ◽  
2007 ◽  
Vol 38 (6) ◽  
pp. 680-683 ◽  
Author(s):  
Ana María Díez-Navajas ◽  
Charles Greif ◽  
Anne Poutaraud ◽  
Didier Merdinoglu
Keyword(s):  
Plasmopara Viticola ◽  
Fluorescent Staining ◽  
Infection Structures ◽  
Leaf Tissues ◽  
Staining Techniques
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