scholarly journals COMPARISON OF ZIEHL-NEELSEN, KINYOUN’S AND FLUORESCENT STAINING FOR DETECTION OF MYCOBACTERIUM TUBERCULOSIS IN SPUTUM SAMPLES BEFORE AND AFTER PETROFF’S CONCENTRATION TECHNIQUE

2018 ◽  
Vol 11 (4) ◽  
pp. 110
Author(s):  
Neelu Sree P ◽  
Terin J ◽  
Himadri Dutta ◽  
Kalyani M
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Sputum Sample ◽  
Diagnostic Value ◽  
Fluorescent Staining ◽  
Concentration Technique ◽  
Before And After ◽  
Auramine O ◽  
Staining Methods ◽  
Staining Techniques ◽  
Microscopy Examination

 Objectives: The objectives of the study were to find out the presence of Mycobacterium tuberculosis in sputum samples using Ziehl-Neelsen (ZN), Kinyoun’s, and (auramine) fluorescent staining and to compare the three staining techniques with and without Petroff’s concentration and to find out the most preferable staining of M. tuberculosis. Methods: Sputum sample was collected and concentrated by 4% NaOH (Petroff’s concentration). Microscopic examination of the sample was done before concentration and after concentration by ZN staining, Kinyoun’s staining, and fluorescent staining (Auramine). Grading of acid-fast bacilli (AFB) by three staining was done before and after concentration according to RNTCP guidelines.Results: Total of 452 sputum samples were collected and subjected to microscopy examination by ZN, Kinyoun’s, and fluorescent staining methods to compare the presence or absence of AFB with or without concentration. Among the 452 sputum samples, total of 67 (15.6%) sputum samples were positive for the presence of AFB. Majority 40.3% (n=21) of TB positive patients were observed in the age group 51–60 years. The results of Auramine-O staining showed positive diagnoses in 15.9% of the samples; sensitivity was 100% and specificity 95.6%.Conclusion: The use of fluorescent staining significantly increases the diagnostic value of the smear, particularly where there are low-density bacilli which may escape detection on ZN stained smears.

scholarly journals Detection of drug-resistant Mycobacterium tuberculosis from TB suspected diabetic patients by GeneXpert MTB/RIF method

2015 ◽  
Vol 4 (1) ◽  
pp. 9-12
Author(s):  
Asmaul Husna ◽  
Tushar Kanti Dhar ◽  
Saurab Kishore Munshi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Multidrug Resistant ◽  
Diabetic Patients ◽  
Drug Resistant ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Mdr Tb ◽  
Auramine O ◽  
Staining Methods ◽  
Sensitivity Specificity

Multidrug-resistant tuberculosis (MDR-TB) has become one of the major public health menace in the developing countries which have destructive consequences in immunocompromised patients. Rapid detection of such cases is indispensable to initiate the early and proper treatment of the infected individuals. Present study was conducted to determine the frequency of drug resistant Mycobacterium tuberculosis among the TB suspected diabetic patients employing GeneXpert MTB/RIF method. A total of 1311 sputum specimens were tested among which 154 (11.7%), 109 (8.3%) and 92 (7%) were found to carry Mycobacterium tuberculosis by GeneXpert, auramine O staining and Zeihl-Neelsen (Z-N) staining methods, consecutively. The relative positivity of the GeneXpert method was 40.3% and 29.2% higher than that of Z-N and auramine O staining methods. The sensitivity, specificity and accuracy of GeneXpert method were found to be higher when compared to these microscopic techniques. An estimation of 12.9% (n=132) of the positive cases was found among the diabetic patients (n=1027), whereas 7.7% (n=22) of the non-diabetic patients (n=284) showed positivity by GeneXpert method. Four (2.6%) among the MTB positive cases exhibited rifampicin resistance of which 3 were diabetic patients. DOI: http://dx.doi.org/10.3329/sjm.v4i1.22754 Stamford Journal of Microbiology, Vol.4(1) 2014: 9-12

scholarly journals Use of fluorescein diacetate (FDA) staining to detect viable Mycobacterium tuberculosis

2012 ◽  
Vol 11 (4) ◽  
pp. 322-330 ◽  
Author(s):  
Shamima Islam ◽  
Farjana Rahman ◽  
Saurab Kisore Munshi ◽  
Jewel Ahmed ◽  
S M Mostafa Kamal ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Treatment Failure ◽  
Medical Science ◽  
Multidrug Resistant ◽  
Fluorescein Diacetate ◽  
Drug Resistant ◽  
Drug Resistant Tuberculosis ◽  
Resistant Tuberculosis ◽  
Multidrug Resistant Tuberculosis ◽  
Auramine O

Objective: Drug resistant tuberculosis has long been a common problem prevailing in developing countries including Bangladesh. Present study focused on the rapid identification of live Mycobacterium tuberculosis among treatment failure cases.Materials and Methods: Sputum samples from a total of 100 category-I and category-II treatment failure cases, assumed as multidrug resistant tuberculosis, were studied through fluorescein diacetate (FDA) staining under light emitting diode (LED) fluorescence microscope. Considering culture method as gold standard, we also compared the results of FDA staining with that of auramine O staining.Results: A total of 85% acid-fast bacilli were detected by FDA staining, 82% by auramine O staining and a total of 85% isolates were detected in Lowenstein-Jensen (LJ) culture. The sensitivity of FDA staining (96.47%) was estimated to be slightly higher than that of auramine O staining (91.76%). Moreover,76.47% cases were detected as multidrug resistant tuberculosis (MDR-TB). Conclusion: Taken together, FDA staining method has been proposed to be appropriate for the rapid diagnosis of drug resistant tuberculosis. DOI: http://dx.doi.org/10.3329/bjms.v11i4.12605 Bangladesh Journal of Medical Science Vol. 11 No. 04 Oct’12

scholarly journals Fibreoptic Bronchoscopy in the Critically Ill: A Prospective Study of its Diagnostic and Therapeutic Value

1992 ◽  
Vol 20 (4) ◽  
pp. 464-469 ◽  
Author(s):  
R. Bellomo ◽  
E. Tai ◽  
G. Parkin
Keyword(s):  
Prospective Study ◽  
Critically Ill ◽  
Lower Lobe ◽  
Major Complication ◽  
Diagnostic Value ◽  
Fibreoptic Bronchoscopy ◽  
Clinical Assessments ◽  
Therapeutic Value ◽  
Before And After ◽  
A Prospective Study

Aim A prospective study was undertaken to assess the diagnostic value and therapeutic usefulness of fibreoptic bronchoscopy in the critically ill. Method Fifty-six bronchoscopies were performed in fifty patients. Biochemical, radiological, microbiological and clinical assessments were made before and after each procedure. Results Eighteen fibreoptic bronchoscopies were performed for therapeutic indications (32.1%) of which ten (55.6%) yielded a useful outcome. Thirty-eight bronchoscopies were for diagnostic purposes (67.8%) of which 22 (5 7.9%) were clinically useful. Broncho-alveolar lavage was performed in twenty-eight cases (50%) and it led to a clinically useful diagnosis in 17 (60.7%). There was no major complication. A subgroup of patients was defined (persistent left lower lobe collapse or consolidation following thoracic or abdominal surgery) in whom fibreoptic bronchoscopy usually did not yield a useful outcome. Conclusion The use of fibreoptic bronchoscopy in the Intensive Care Unit, in combination with the technique of broncho-alveolar lavage, results in a clinically useful outcome in the majority of cases. Fibreoptic bronchoscopy is an effective and safe diagnostic and therapeutic tool in critically ill patients.

scholarly journals The Rule of Histology in the Diagnosis of Periprosthetic Infection: Specific Granulocyte Counting Methods and New Immunohistologic Staining Techniques may Increase the Diagnostic Value

2016 ◽  
Vol 10 (1) ◽  
pp. 457-465 ◽  
Author(s):  
Friedrich Boettner ◽  
Gabriele Koehler ◽  
Alexander Wegner ◽  
Tom Schmidt-Braekling ◽  
Georg Gosheger ◽  
...  
Keyword(s):  
Periprosthetic Infection ◽  
High Specificity ◽  
Diagnostic Value ◽  
Lower Sensitivity ◽  
Specific Staining ◽  
Tissue Samples ◽  
Hip And Knee Arthroplasty ◽  
Staining Techniques ◽  
Low Sensitivity ◽  
Intraoperative Test

Objective: The current study investigates the diagnostic accuracy of the criteria described for frozen sections and whether modern leukocyte specific staining techniques including leukocyte peroxidase and Naphtol-AS-D-chloroacetate-esterase will improve the accuracy of the intra-operative histology. Method: 77 patients undergoing revision total hip and knee arthroplasty were included in this retrospective study. Patients were grouped into septic and aseptic based on intraoperative cultures. Tissue samples were analyzed utilizing the Mirra, Feldman, Lonner, Banit and Athanasou criteria. Results: An experienced pathologist had a high specificity (96%), but rather low sensitivity (57%) diagnosing infection. By using the Banit-, Mirra-, or Athanasou-criteria the sensitivity is increased to 0.90. The Feldman- and Lonner-criteria have a lower sensitivity (0.48 and 0.38), however, an increased specificity of 0.96 and 0.98, respectively. The Banit cut off has the highest accuracy (86%). MPOX and NACE staining increased the sensitivity and accuracy up to 100% and 92% respectively. Conclusion: Banit’s cut off is the most accurate histologic criteria to diagnose infection. Modern leukocyte specific staining techniques slightly improve the accuracy. The synovial fluid white blood cell count appears to be the most accurate intraoperative test.

scholarly journals Diagnosis and surgical treatment of liver echinococcosis

2021 ◽  
Vol 26 (4) ◽  
pp. 113-117
Author(s):  
V.O. Shaprinskyi  ◽  
O.O. Vorovskyi ◽  
O.A. Kaminskyi ◽  
Ya.M. Pashynskyi
Keyword(s):  
Blood Loss ◽  
Pleural Cavity ◽  
Abdominal Cavity ◽  
Diagnostic Value ◽  
Results Of Treatment ◽  
Before And After ◽  
Residual Cavity ◽  
Right Lobe ◽  
The Right ◽  
Duration Of Operation

The results of treatment of 72 patients with echinococcosis of the liver were analyzed, women – 62 (86.2%), men – 10 (13.8%). Primary echinococcosis was detected in 69 (95.8%) patients, secondary – in 3 (4.2%). Among instrumental research methods, ultrasound and computed tomography examination were of diagnostic value. Single liver cysts were found in 63 (87.5%) patients, multiple – in 9 (12.5%). Among patients with solitary cysts, the right lobe was more often affected than the left – 48 (66.7%) vs 24 (33.3%) cases. Echinococcosis of central localization was less common and was noted in 8 (11.1%) cases. Echinococcosis complications were observed in 16 (22.2%) patients. Among them, most often there were suppurations of the cyst – in 13 (18.1%); a bursting of the cyst into the free abdominal cavity – in 1 (1.4%), in the pleural cavity – 1 (1.4%), in the biliary tract – in 1 (1.4%). Partial or complete liming of the hand was observed in 12 (16.7%) patients. In 20 (27.8%) cases, the operation was performed from the upper median access, in 42 (58.3%) – from oblique hypochondria accesses by Kocher or by Fedorov. Pericystectomy was performed in 48 (66.7%) patients, in 8 (11.1%) patients underwent resections of liver segments with an echinococcal cyst, in 4 (5.6%) – cyst opening with removal of contents and treatment of its cavity. Laparoscopic echinococectomy was used in 12 (16.7%) patients. In the postoperative period complications were observed in 16 (22.2%) patients. The use of the welding electrocoagulator EK-300M "Swarmed" in the thermal rehabilitation of the walls of the residual cavity after echinococectomy allowed to reduce blood loss from 2200±210 ml to 250±50 ml. With the use of laparoscopic echinococectomy, intraoperative blood loss was reduced by 9 times (р=0.0001); duration of operation – 2 times (р<0.05), stay in hospital – 3.3 times (р=0.002). There were no fatal outcomes. Before and after operation antirelapse antiparasitic therapy with albendazole (Vormil) was performed in two cycles of 28 days, separated by a 14-day break. The dose at body weight over 60 kg was 400 mg 2 times a day, and for less than 60 kg the drug was calculated at a rate of 15 mg/kg/day. There were 2 (2.8%) cases of relapse, there was no mortality.

scholarly journals Annotated Sequence of Mycobacterium tuberculosis MTBR3/09 Isolated from a Sputum Sample in Malaysia

2016 ◽  
Vol 4 (3) ◽  
Author(s):  
Rahizan Issa ◽  
Valentinus H. Seradja ◽  
Mohd Khairul Hafizi Abdullah ◽  
Hatijah Abdul
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Genome Sequence ◽  
Sputum Sample

This is a report of the annotated genome sequence of Mycobacterium tuberculosis MTBR3/09. The organism was isolated from a sputum sample in Malaysia.

scholarly journals Treatment of the mandibular shift in an adult woman and the diagnostic value of joint space index: a case report

2020 ◽  
Vol 25 (1) ◽  
Author(s):  
Kai Xia ◽  
Wentian Sun ◽  
Liyuan Yu ◽  
Xinqi Huang ◽  
Zhihe Zhao ◽  
...  
Keyword(s):  
Cone Beam Ct ◽  
Joint Space ◽  
Diagnostic Value ◽  
Outcome Evaluation ◽  
Case Presentation ◽  
Arch Form ◽  
Before And After ◽  
Condyle Position ◽  
Diagnostic Approaches ◽  
Skeletal Asymmetry

Abstract Background Mandibular deviations are common clinical complaints. The orthodontic or orthognathic treatment of mandibular deviations is tricky because a comprehensive diagnosis, especially a functional one, is difficult to make. A inaccurate diagnosis may lead to a compromised and unstable treatment outcome. Case presentation This article describes the diagnosis and treatment of a woman with a mandibular deviation and facial skeletal asymmetry. By eliminating the disharmony of the arch form with elastics and bite turbos, her esthetic and functional outcomes improved. Cone-beam CT (CBCT) and Joint Space Index (JSI) analyses served as the diagnostic approaches and outcome evaluation methods before and after treatment. Conclusions A condyle position displacement could be an indication of functional deviation. JSI analysis is a quantitative and convenient choice to compare condyle relative positions.

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