scholarly journals Effect of Poultry Manure on Pepper Veinal Mottle Virus (PVMV), Yield and Agronomic Parameters of Pepper (Capsicum annuum) in Nigeria

2007 ◽  
Vol 1 (2) ◽  
Author(s):  
A A Fajinmi ◽  
C A Adebode
Keyword(s):  
Capsicum Annuum ◽  
Poultry Manure ◽  
Mottle Virus ◽  
Pepper Veinal Mottle Virus

scholarly journals Antioxidant Responses of Three Pepper (Capsicum annuum) Varieties against Pepper veinal mottle virus

2019 ◽  
pp. 1-10
Author(s):  
Léon W. Nitiema ◽  
Pierre A. E. D. Sombié
Keyword(s):  
Superoxide Dismutase ◽  
Protein Content ◽  
Capsicum Annuum ◽  
Biochemical Parameters ◽  
Chili Pepper ◽  
Mottle Virus ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pepper Veinal Mottle Virus ◽  
Significant Difference

Aims: This study aimed to investigate the changes in antioxidant activity and protein content between non-infected and infected leaves of three Capsicum annuum varieties against Pepper veinal mottle virus. Materials and Methods: Pepper veinal mottle virus isolated from infected pepper plants was inoculated to three healthy varieties of pepper (Pepper Narval, Yolo Wonder and Chili pepper) by gently rubbing on the leaves of 14-day-old seedlings. Control peppers of each variety were treated in the same way with distilled water. The infection of inoculated plants was confirmed by the enzyme-linked immunosorbent assay method. Control and infected leaves were collected 21 days after inoculation (when symptoms manifested) and used for biochemical analyses. Change in different biochemical parameters (catalase, superoxide dismutase, malondialdehyde and protein) in infected pepper plant was observed compared to control non-infected ones. Results: Catalase and superoxide dismutase activities were increased in Pepper Narval and Pepper Yolo Wonder infected leaves compared to non-infected, while a significant decrease was observed in infected Chili pepper compared to control. Higher malondialdehyde content was found in Pepper Yolo Wonder and Chili pepper infected leaves (P < 0.05) than control while a non-significant difference was shown between the infected and non-infected of Pepper Narval variety (P > 0.05). Infected Chili pepper showed high protein content compared to control (P < 0.05). An opposite trend was observed in pepper Narval and Yolo Wonder varieties (P < 0.05). Conclusion: The results of this study showed that Pepper veinal mottle virus infection induces changes in antioxidant enzymes activities, malondialdehyde and total protein levels. These biochemical components were greatly expressed differentially between Pepper veinal mottle virus infected and non-infected in Pepper Yolo Wonder variety. Further studies with more biochemical parameters may contribute to improve the pepper tolerance mechanism to Pepper veinal mottle virus in a breeding program.

First Report of Pepper mottle virus on Capsicum annuum in Japan

2003 ◽  
Vol 69 (5) ◽  
pp. 348-350 ◽  
Author(s):  
Yoshihiro Ogawa ◽  
Kyoji Hagiwara ◽  
Hisashi Iwai ◽  
Shoichi Izumi ◽  
Kei Arai
Keyword(s):  
Capsicum Annuum ◽  
Mottle Virus ◽  
Pepper Mottle Virus ◽  
First Report

scholarly journals Antioxidant and Oxidant Levels of Pepper (Capsicum annuum cv. ‘Charlee’) Infected with Pepper Mild Mottle Virus

2011 ◽  
Vol 39 (2) ◽  
pp. 58 ◽  
Author(s):  
Murat DIKILITAS ◽  
Mehmet Ertugrul GULDUR ◽  
Ahmet DERYAOGLU ◽  
Ozcan EREL
Keyword(s):  
Vitamin C ◽  
Capsicum Annuum ◽  
Total Antioxidant Status ◽  
Mottle Virus ◽  
Stress Index ◽  
Pepper Mild Mottle Virus ◽  
Free Phenol ◽  
Biochemical Analyses ◽  
Commercial Kits

Total antioxidant status (TAS), total oxidant status (TOS), as well as total phenol (TP), free phenol (FP), conjugated phenol (CP), and the vitamin C content of pepper plants (Capsicum annuum cv. ‘Charlee’) infected with Pepper mild mottle virus (PMMoV) were determined via a novel automated antioxidant system using test kits. PMMoV was first identified in Turkey in 1994 in commercial pepper (Capsicum annuum cv. ‘Charlee’) fields and caused an outbreak in southern Turkey by 2006, especially in greenhouses. Infected plants were confirmed by ELISA using commercial kits for the virus. The infected plants had stunted growth; and leaves and fruits became mottled, puckered, malformed, deformed, and marked by off-colored sunken areas. Fruits expressing virus symptoms were utilized for biochemical analyses. The percent ratio of TOS to TAS was calculated as the oxidative stress index (OSI). The TAS level and the vitamin C content were found to be significantly lower in infected peppers as compared to healthy peppers. However, TOS, OSI, TP, FP, and CP levels were significantly higher in infected pepper fruits than in healthy ones. In this study, it has been demonstrated that low levels of TAS and high levels of TOS, as well as high OSI status, reflects the quality of peppers. Using this method, the quality of fruits and the condition of plants can be determined in advance of stress development.

scholarly journals Use of an Infectious cDNA Clone of Pepper Veinal Mottle Virus to Confirm the Etiology of a Disease in Capsicum chinense

2020 ◽  
Vol 110 (1) ◽  
pp. 80-84 ◽  
Author(s):  
Weiyao Hu ◽  
Li Qin ◽  
Haixia Yan ◽  
Weiguo Miao ◽  
Hongguang Cui ◽  
...  
Keyword(s):  
Cdna Clone ◽  
Hainan Island ◽  
Mottle Virus ◽  
Reverse Genetic ◽  
Capsicum Chinense ◽  
Genetic Tool ◽  
Pepper Veinal Mottle Virus ◽  
Virus Progeny ◽  
Local Germplasm ◽  
Pepper Cultivar

The pepper cultivar Yellow Lantern, one of the spiciest pepper varieties, is a local germplasm of Capsicum chinense, cultivated exclusively on Hainan Island, China. However, this variety is susceptible to viral diseases that severely affect its production. In this study, we report that pepper veinal mottle virus (PVMV) is associated with foliar chlorosis and rugosity symptoms in Yellow Lantern. To verify this correlation, we constructed a full-length cDNA clone of a PVMV isolate named HNu. The virus progeny derived from the cDNA clone replicated and moved systemically in the pepper, inducing the same symptoms as those induced by PVMV-HNu in Yellow Lantern peppers in the field. The results support that PVMV-HNu is the causal agent of foliar chlorosis and rugosity disease in Yellow Lantern. This knowledge will help in the diagnosis and prevention of disease caused by PVMV. Furthermore, the cDNA clone serves as a reverse genetic tool to study the molecular pathogenesis of PVMV.

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