scholarly journals Antioxidant Responses of Three Pepper (Capsicum annuum) Varieties against Pepper veinal mottle virus

2019 ◽  
pp. 1-10
Author(s):  
Léon W. Nitiema ◽  
Pierre A. E. D. Sombié
Keyword(s):  
Superoxide Dismutase ◽  
Protein Content ◽  
Capsicum Annuum ◽  
Biochemical Parameters ◽  
Chili Pepper ◽  
Mottle Virus ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Pepper Veinal Mottle Virus ◽  
Significant Difference

Aims: This study aimed to investigate the changes in antioxidant activity and protein content between non-infected and infected leaves of three Capsicum annuum varieties against Pepper veinal mottle virus. Materials and Methods: Pepper veinal mottle virus isolated from infected pepper plants was inoculated to three healthy varieties of pepper (Pepper Narval, Yolo Wonder and Chili pepper) by gently rubbing on the leaves of 14-day-old seedlings. Control peppers of each variety were treated in the same way with distilled water. The infection of inoculated plants was confirmed by the enzyme-linked immunosorbent assay method. Control and infected leaves were collected 21 days after inoculation (when symptoms manifested) and used for biochemical analyses. Change in different biochemical parameters (catalase, superoxide dismutase, malondialdehyde and protein) in infected pepper plant was observed compared to control non-infected ones. Results: Catalase and superoxide dismutase activities were increased in Pepper Narval and Pepper Yolo Wonder infected leaves compared to non-infected, while a significant decrease was observed in infected Chili pepper compared to control. Higher malondialdehyde content was found in Pepper Yolo Wonder and Chili pepper infected leaves (P < 0.05) than control while a non-significant difference was shown between the infected and non-infected of Pepper Narval variety (P > 0.05). Infected Chili pepper showed high protein content compared to control (P < 0.05). An opposite trend was observed in pepper Narval and Yolo Wonder varieties (P < 0.05). Conclusion: The results of this study showed that Pepper veinal mottle virus infection induces changes in antioxidant enzymes activities, malondialdehyde and total protein levels. These biochemical components were greatly expressed differentially between Pepper veinal mottle virus infected and non-infected in Pepper Yolo Wonder variety. Further studies with more biochemical parameters may contribute to improve the pepper tolerance mechanism to Pepper veinal mottle virus in a breeding program.

scholarly journals N-TERMINAL PRO-BRAIN NATRIURETIC PEPTIDE (NT-proBNP) IN STAGE 1 AND STAGE 2 HYPERTENSION PATIENTS

2019 ◽  
Vol 1 (2) ◽  
pp. 65-67
Author(s):  
Supriati Wila Djami
Keyword(s):  
Left Ventricular ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Hypertensive Patients ◽  
Hypertensive Group ◽  
Undiagnosed Hypertension ◽  
Increased Risk ◽  
Study Results ◽  
Significant Difference ◽  
Stage 1

The increased levels of NT-proBNP in the blood occur when heart function, especially the left ventricular muscle chambers of the heart increases. Therefore, NT-proBNP is used as a biomarker to detect heart failure.The level of N Terminal – Pro Brain Natriuetic Peptide was independently associated with an increased risk of hypertension. This study aimed to determine the difference of NT-proBNP serum levels and the correlation between the levels of NT-proBNP in patients with stage 1 and stage 2 hypertension. This research was conducted at RSUP dr. Wahidin Sudirohusodo in August - September 2018. The study used a cross-sectional design with the total of 72 hypertensive patients, who had met the inclusive criteria. NT-proBNP levels were measured using the ELISA (Enzyme Linked Immunosorbent Assay) method.  The collected data was processed using Mann Whitney Different Test and Spearman's rho Correlation Test. The study results indicated that the level of NT-proBNP in the hypertensive patients with stage 2 was higher and significantly different (p = <0.001) compared to stage 1 hypertensive patients. NT-proBNP levels were higher in the hypertensive group of >6 years than in the hypertensive group <6 years. There were significant differences between the two groups statistically (p=0.010). It can be Conclude that there is a significant difference in the levels of NT-proBNP with a degree of hypertension where NT-proBNP levels were higher in patients with stage 2 hypertension compared to stage 1 hypertension, although  there was not statistically significant correlation between levels of NT-proBNP with Hypertension degree. Further research was needed to determine the relationship of NT-proBNP levels with the degree of hypertension, which can confirm the diagnosis, especially in patients with hypertension. Also, it is suggested to consider the accuracy of the data length of a patient suffering from undiagnosed hypertension.

scholarly journals Agricultural soils strengthening employing humic acids and its effect on plant growth chilli pepper and eggplant

2018 ◽  
Author(s):  
Fredy Colpas- Castillo ◽  
Arnulfo Tarón Dunoyer ◽  
Jairo Mercado Camargo
Keyword(s):  
Humic Acid ◽  
Plant Growth ◽  
Capsicum Annuum ◽  
Humic Acids ◽  
Agricultural Soils ◽  
Solanum Melongena ◽  
Chili Pepper ◽  
Growth Promoter ◽  
Chilli Pepper ◽  
Significant Difference

The Humic substance has been recognized widely as a plant growth promoter because they induce changes in root, architecture and dynamics of growth which result in larger root size. The aim of this study was evaluating the effect Humic acids on the chili pepper (Capsicum annuum) and eggplant (Solanum melongena) plants growing in soils fertilized with urea. 200 g of soil were incorporated to the fertilizer once and the Humic acid in concentrations of 0.05%, 0.1% and 0.2%. All measurements growths were carried out during 45 days growth. The finding shows that all treatments cause significant increases (p < 0.05) in the total area of roots. For the chili pepper, the multiple comparison tests of Tukey and Dunnett showed that there was a statistically significant difference between the control soils and the soils containing 0.1% and 0.2% of Humic acid. By the opposite, no statistical differences (p > 0.05) were observed among control soil and those containing 0.05 % of Humic acid. Concerning the eggplant, significant differences were established (p<0.05) between the control and samples incorporated with 0.1% and 0.2% of Humic acid. The Humic acids act as a growth biostimulant in chili pepper plants (Capsicum annuum) and eggplant (Solanum melongena).

scholarly journals Alterations in Serum Adropin, Adiponectin, and Proinflammatory Cytokine Levels in OSAS

2020 ◽  
Vol 2020 ◽  
pp. 1-7
Author(s):  
Hakan Celikhisar ◽  
Gulay Dasdemir Ilkhan
Keyword(s):  
Proinflammatory Cytokine ◽  
Sleep Apnea Syndrome ◽  
Density Lipoprotein ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Apnea Hypopnea Index ◽  
Obstructive Sleep ◽  
Significant Difference ◽  
Cytokine Levels

Objective. The present study was planned to examine the relationships between obstructive sleep apnea syndrome (OSAS) and the newly revealed adipokines adropin and adiponectin concentrations that display significant metabolic and cardiovascular functions and the levels of proinflammatory cytokine levels. Method. A total of 166 overweight and obese male patients with a body mass index (BMI) >27 kg/m2 were included in the study. Among study participants, 84 were recently diagnosed with OSAS by polysomnography with an apnea-hypopnea index (AHI) ≥5, and 82 were nonapneic with normal polysomnography (AHI<5) findings. The serum adropin and adiponectin levels of all cases were analyzed via the enzyme-linked immunosorbent assay method. Serum interleukin-1 (IL-1) beta and tumor necrotizing factor-alpha (TNF-alpha) levels were determined using Luminex cytokine multiplex analyses. Results. The mean age of the OSAS patients was 50.9 ± 5.7 years and BMI was 32.4 ± 6.0 kg/m2, and there was no statistically significant difference determined with the control group (49.3 ± 5.8 years and 30.6 ± 5, 6 kg/m2) (p>0.05). There were no statistically significant differences between the OSAS and control groups concerning total cholesterol, triglyceride, low-density lipoprotein (LDL), high-density lipoprotein (HDL), and glucose levels. Adiponectin was lower in the OSAS group at a statistically significant level in comparison with the control group and was related at a statistically significant level to OSAS intensity. Adropin concentration was determined to be higher in the OSAS group at a statistically significant level in comparison with the control group. Conclusion. The results of our study suggest that increased adropin concentration may be an indicator of endothelium dysfunction in OSAS patients. Serum adropin and adiponectin levels may be new bioindicators used for diagnosis and risk assessment in OSAS patients.

scholarly journals HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA

2019 ◽  
Vol 1 (2) ◽  
pp. 59-67
Author(s):  
Muhamad Arif Budiman ◽  
Mohamad Sadikin ◽  
Ani Retno Prijanti
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Binding Protein ◽  
Serum Folate ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ligand Binding Assay ◽  
Elisa Method ◽  
Folate Binding Protein ◽  
Significant Difference

Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method.Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk.Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine’s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide.Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363.Conclusion:  ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).

scholarly journals Effect of Air Pollution on Salivary Interleukin-8 Levels in Children

2016 ◽  
Vol 7 (4) ◽  
pp. 175-178
Author(s):  
Solmaz Pourzare Mehrbani ◽  
Zohreh Babaloo ◽  
Zahra Jamali ◽  
Tahmoores Abdollahian ◽  
Hosein Eslami ◽  
...  
Keyword(s):  
Air Pollution ◽  
Interleukin 8 ◽  
Assay Method ◽  
Pollution Level ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
P Value ◽  
Case Group ◽  
Group I ◽  
Significant Difference

ABSTRACT Background Air pollution in cities has always been a permanent and serious threat for the health of society and the environment, influencing the health of different body organs and systems both acutely and chronically. Therefore, the aim of this study is to investigate the effect of air pollution in saliva Interleukin-8 (IL-8) levels in children. Materials and methods A total of 88, 8- to 10-year-old children were included in this descriptive cross-sectional study. Group I, the case group, included 44 children from Tabriz (Iranian Azerbaijan), and group II, the control group, included 44 children from Kalibar Town (of Tabriz). Both groups in this study had a similar economic status. Following sampling and selection of individuals, for measuring the levels of saliva IL-8, ELISA (enzyme-linked immunosorbent assay) method was used. To investigate the difference between the means of the groups, independent t test or its nonparametric equivalent, i.e., Man—Whitney test and Statistical Package for the Social Sciences (SPSS) 21 were used. In this study, p-value lower than 0.05 was considered statistically significant. Results The results of this study indicate a significant difference in the levels of saliva IL-8 between the two groups, where the mean level of saliva IL-8 is greater in children residing in the region with higher air pollution level than in the children dwelling in the region with a greater air pollution level (p = 0.001). Conclusion The results obtained from this study indicated that the level of saliva IL-8 increases in regions with air pollution, potentially causing several mouth problems in children. How to cite this article Mehrbani SP, Babaloo Z, Jamali Z, Abdollahian T, Eslami H, Sobhani N. Effect of Air Pollution on Salivary Interleukin-8 Levels in Children. World J Dent 2016;7(4):175-178.

175 EFFECTS OF GLUCURONIC ACID AND N-ACETYL-D-GLUCOSAMINE SUPPLEMENTATION ON THE PERIVITELLINE SPACE DURING THE IN VITRO MATURATION OF PORCINE OOCYTES

2017 ◽  
Vol 29 (1) ◽  
pp. 196
Author(s):  
J. Z. Current ◽  
B. D. Whitaker
Keyword(s):  
Hyaluronic Acid ◽  
Embryonic Development ◽  
Glucuronic Acid ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cortical Granule ◽  
Perivitelline Space ◽  
Significant Difference ◽  
Pronuclear Formation

Pig oocytes fertilized in vitro experience high polyspermic penetration rates due to inadequate cortical granule exocytosis into reduced perivitelline space (PVS) thickness. The objective of this study was to minimize polyspermic penetration by increasing the PVS thickness through supplementation of its hyaluronic acid components, glucuronic acid (GA), and N-acetyl-d-glucosamine (GlcNAc) during maturation. Oocytes were supplemented during the first 24 h or second 24 h of maturation with 0.01 mM GA and 0.01 mM GlcNAc and then evaluated for nuclear maturation (n = 200), PVS thickness (n = 245), and the amount of hyaluronic acid (n = 245) present. The PVS thickness was determined at the equatorial plane of the oocyte using a micrometer. Hyaluronic acid concentrations were determined using an enzyme-linked immunosorbent assay method. Oocytes (n = 800) were fertilized using frozen-thawed semen and evaluated for fertilization characteristics and subsequent embryonic development at 48 and 144 h for cleavage and blastocyst formation, respectively. The PVS thickness was significantly thicker (P < 0.05) with oocytes supplemented with 0.01 mM GA and 0.01 mM GlcNAc during the first half of maturation (3.20 ± 0.29) and all of maturation (2.78 ± 0.21) compared with no supplementation (2.22 ± 0.13) and supplementation during only the second half of maturation (2.02 ± 0.16). The amount of hyaluronic acid present at 24 h of maturation was significantly greater (P < 0.05) in oocytes supplemented with the PVS components (2.03 ± 0.07 pg/oocyte) compared with no supplementation (0.21 ± 0.02 pg/oocyte). At the end of maturation, oocytes supplemented with 0.01 mM GA and 0.01 mM GlcNAc during the entire maturation had significantly greater (P < 0.05) amounts of hyaluronic acid present (4.16 ± 0.19 pg/oocyte) compared with all other groups. There was no significant difference in penetration rates between the groups. Polyspermic penetration was significantly less (P < 0.05) in oocytes supplemented with 0.01 mM GA and 0.01 mM GlcNAc during the first half of maturation compared with no supplementation or supplementation during only the second half of maturation. Oocytes supplemented with 0.01 mM GA and 0.01 mM GlcNAc during the first half of maturation (87.36 ± 4.01) compared with no supplementation (76.47 ± 5.67) or supplementation during only the second half of maturation (80.23 ± 3.21) had significantly higher percentages (P < 0.05) of male pronuclear formation by 12 h after IVF. Supplementing 0.01 mM GA and 0.01 mM GlcNAc during maturation significantly increased (P < 0.05) the percentage of cleaved embryos by 48 h after IVF and the percentage of those reaching the blastocyst stage by 144 h after IVF compared with those that were not supplemented during maturation (55.00 ± 6.43, 20.00 ± 6.16). There were no significant differences between the supplementation treatment groups at 48 or 144 h after IVF. These results indicate that supplementing GA and GlcNAc to the media during maturation, specifically during the first 24 h, decreases polyspermic penetration by increasing PVS thickness, hyaluronic acid amount, and male pronuclear formation, which improves subsequent embryonic development.

Study of correlation between biochemical indicators and radiological diagnostic parameters of breast cancer

2020 ◽  
Vol 65 (12) ◽  
pp. 738-743
Author(s):  
Ilaha Nadir Orujova ◽  
G. I. Azizova ◽  
I. A. Gafarov ◽  
A. H. Orujov
Keyword(s):  
Breast Cancer ◽  
Biochemical Parameters ◽  
Ultrasound Examination ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Control Group ◽  
Regional Lymph Nodes ◽  
X Ray ◽  
Radiological Diagnostics ◽  
Tnf Α

The aim of this work was to study the correlation between some biochemical parameters and parameters of radiological diagnostics for early diagnosis of breast cancer. 76 patients with breast cancer were examined. In 48 of them was diagnosed breast cancer, in 28 of them was diagnosed benign breast neoplasms. The age of patients ranged from 18 to 79 years. The control group consisted of 16 healthy women. Oncological markers (CEA, CA 15-3), some pro-inflammatory and inflammatory cytokines (IL-2, IL-6, IL-8, IL-10 and TNF-α) and lactoferrin were determined in serum by using enzyme-linked immunosorbent assay method. All patients underwent ultrasound with a combination of Doppler and X-ray mammography. Ultrasound examination assessed the estimation of tumor size, contours, echogenicity, echostructure, the presence and nature of vascularization of breast tumors, and also assessed the location of regional lymph nodes. During mammography, the contours and sizes of the detected tumor were determined, and the presence of microcalcifications was also taken into account. The results of the study showed that a statistically positive correlation between some biochemical parameters and parameters of radiological diagnostics was established.

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