scholarly journals Antibiotic resistance pattern of methicillin-resistant Staphylococcus aureus isolated from children under the age of five Years in Anambra State

2019 ◽  
Vol 36 (1) ◽  
pp. 213
Author(s):  
I Emeka-Nwabunnia ◽  
K Ejigeme ◽  
O.I. Oguoma
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Resistance Pattern ◽  
Methicillin Resistant ◽  
Anambra State ◽  
Antibiotic Resistance Pattern

Genetic diversity of healthcare-associated methicillin-resistant Staphylococcus aureus isolates from Southern Iran

2021 ◽  
Author(s):  
Sorour Farzi ◽  
Mohsen Rezazadeh ◽  
Ahmadreza Mirhosseini ◽  
Mohammad Amin Rezazadeh ◽  
Farhan Houshyar ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Molecular Epidemiology ◽  
Resistance Pattern ◽  
Spa Typing ◽  
Methicillin Resistant ◽  
Antibiotic Resistance Pattern ◽  
Southern Iran ◽  
Resistance Patterns ◽  
Healthcare Associated

AbstractStaphylococcus aureus is a common pathogen causing hospital infections. The increasing rate of healthcare-associated infections caused by methicillin-resistant Staphylococcus aureus (MRSA) in developing countries has led to many public health problems. This study aimed to investigate the molecular epidemiology as well as the antibiotic resistance pattern of clinical isolates of MRSA from Southern Iran. A total of 135 S. aureus isolates were collected from the patients referred to three hospitals in South Iran. The phenotypic and genotypic diagnosis of MRSA isolates was performed by disk diffusion and PCR methods, respectively. The antibiotic resistance pattern for MRSA isolates was performed using Kirby–Bauer method. The molecular epidemiology of isolates was performed by MLST, Spa typing and SCCmec typing. From 135 S. aureus isolates, 50 (37%) MRSA strains were detected from which two different sequence types including ST239 and ST605 were identified. SCCmec type III was the most common profile (50%) and t030 was the predominant spa type (48%) among the strains. The MRSA isolates had the highest resistance to penicillin (100%), tetracycline (88%), levofloxacin (86%), ciprofloxacin (84%), erythromycin (82%), gentamicin (80%), and clindamycin (78%). The results of this study show that the most common genetic type among the MRSA isolates was ST239-SCCmec III/t030. The rapid and timely detection of MRSA and the administration of appropriate antibiotics according to the published antibiotic resistance patterns are essential. Furthermore, the continuous and nationwide MRSA surveillance studies are necessary to investigate clonal distribution and spreading of MRSA from community to hospitals.

scholarly journals Antibiotic resistance pattern of Staphylococcus aureus with reference to MRSA isolates from pediatric patients

2019 ◽  
Author(s):  
Raja Ram Gurung ◽  
Prashanna Maharjan ◽  
Ganga GC
Keyword(s):  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Pediatric Patients ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Current Status ◽  
Clinical Samples ◽  
Resistance Pattern ◽  
Isolation And Identification ◽  
Cross Sectional ◽  
Methicillin Resistant

Abstract Background: Staphylococcus aureus is one of the important superbugs distributed throughout the world. It causes minor skin infections to severe complications including nosocomial infections in both hospitals and community settings. These strains have multi-drug resistant property. Hence, they are difficult to manage which increase health-related costs and simultaneously intensifying the need for new antibiotics. The extent of Methicillin-Resistant Staphylococcus aureus (MRSA) in children is largely unknown. The study determines the current status of S. aureus and MRSA causing various infections in pediatric patients visiting International Friendship Children’s Hospital (IFCH). Methods: A cross-sectional study was conducted among patients visiting a hospital. Various clinical specimens were aseptically collected and processed according to standard microbiological procedures. Isolation and identification of S. aureus were done by microscopy, mannitol fermentation, and coagulase positivity. All identified S. aureus isolates subjected to in-vitro antibiogram by Kirby-Bauer disc diffusion technique adopting Clinical and Laboratory Standards Institute (CLSI) guideline. Isolates resistant to cefoxitin were considered to be MRSA. Whereas, isolates produced D-shaped inhibition zone around clindamycin when kept near erythromycin were considered to be Inducible Clindamycin Resistant (ICR). Results: 672 various types of clinical samples were processed from the microbiology laboratory from June and November 2015. Out of 300 culture positive samples, 52 (17.3%) were S. aureus isolates, among them 39 (75.0%) were found to be MRSA. The D-test showed that Macrolide-Lincosamide-Streptogramin-B (MLSB) phenotype was 15.4%. Conclusion: The study shows the MRSA occurrence is prevalent in pediatric patients and newer classes’ drugs are found more effective than β-lactam drugs to treat S. aureus infection. However, restriction on the indiscriminate use of such drugs may be an effective strategy to control the drug resistance. Keywords: Methicillin-Resistant Staphylococcus aureus (MRSA), Macrolide-Lincosamide-Streptogramin B (MLSB) phenotype, Inducible Clindamycin Resistant (ICR) test or D-zone test, Antibiotic resistance, Nepal

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