Isolation and Identification of Phenol-Degrading Bacteria from Oil-Contaminated Sites

This work is aimed at isolating and identifying phenol-degrading bacteria from oil-contaminated sites. Five soil samples from three auto-mechanic workshops within Katsina metropolis were collected. The samples were analyzed by selective enrichment technique, which resulted in the isolation of four bacterial species. The species were further subjected to the Vitek 2 compact microbiological system analysis. Cupriavidus pauculus, Pontoea spp, Proteus mirabilis 1 and Proteus mirabilis 2 were identified. Result from the present study showed that the bacteria could utilize phenol as their carbon source. Proteus mirabilis 1 and Proteus mirabilis 2 showed lower phenol degradation potential, under similar conditions. Cupriavidus pauculus and Pontoea sp. showed significant increases (p<0.05) in their optical densities. The optical density increment is strongly correlated with increase in colony forming units of the bacteria. This study further showed that the isolates could tolerate high phenol concentrations and may serve as strong putative isolates in bioremediation of phenol-contaminated sites.

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Characterization of hydrocarbon utilizing bacteria in waste engine oil-impacted sites

Highlights in BioScience ◽

10.36462/h.biosci.20218 ◽

2020 ◽

Author(s):

Iniobong Ime James ◽

Mayen Godwin Ben ◽

Agnes Monday Jones ◽

Patience Saturday Akpan ◽

Idorenyin Idorenyin Eka ◽

...

Keyword(s):

Physicochemical Properties ◽

Oil Pollution ◽

Micrococcus Luteus ◽

Bacterial Species ◽

Engine Oil ◽

Soil Physicochemical Properties ◽

Selective Enrichment ◽

Waste Engine Oil ◽

Enrichment Technique ◽

Degrading Bacteria

Changes in soil physicochemical properties and bacterial species present in soil contaminated with waste engine oil were evaluated at three auto-mechanical workshops in Uyo, Nigeria. This work was aimed at isolating and identifying hydrocarbon degrading bacteria from waste engine oil polluted soil, and assessing their hydrocarbon-utilizing ability. Waste engine oil pollution affected soils significantly with increases in soil physicochemical properties, and heterotrophic bacterial population counts. Eight bacterial species Corynebacterium kutscheri, Pseudomonas aeruginosa, Flavobacterium aquatile, Serratia odorifera, Micrococcus agilis, Staphylococcus aureus, Micrococcus luteus and Bacillus substilis were isolated by the selective enrichment technique and screened for hydrocarbon utilization capability in mineral salt media with 1% (v/v) waste engine oil as a sole carbon and energy source. The extent of bacterial growth observed was related to the ability of organisms to biodegrade hydrocarbons present in the medium bacterium species, which showed varying hydrocarbon utilization during the 15 days of incubation. Growth in hydrocarbon medium was the most efficient in cultures of Corynebacterium kutscheri. All isolates also showed variable emulsification ability, with Corynebacterium kutscheri, showing the highest ability. These results demonstrate the presence of indigenous bacteria in hydrocarbon-polluted soils and the potential toward the remediation of hydrocarbons.

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Isolation and partial characterization of organophosphate pesticide degrading bacteria from soil sample of Noakhali, Bangladesh

Bangladesh Journal of Microbiology ◽

10.3329/bjm.v36i1.44263 ◽

2019 ◽

Vol 36 (1) ◽

pp. 17-22

Author(s):

Mahin Reza ◽

Jannatul Fiza ◽

Foysal Hossen ◽

Firoz Ahmed

Keyword(s):

Soil Surface ◽

Sole Source ◽

Growth Study ◽

Mineral Salts ◽

Selective Enrichment ◽

Pseudomonas Spp ◽

Enrichment Technique ◽

Viable Solution ◽

Degrading Bacteria ◽

Visible Growth

Extensive use of organophosphate pesticides particularly malathion can result in pollution of soil, surface water and ground water, and thus disrupts ecosystem byexposing non-target species to its toxicity. Bioremediation with indigenous microorganisms having pesticide utilizing abilities is considered to be a viable solution regarding decontamination of organophosphate residues from pesticide contaminated soil. In this study, we isolated five malathion degrading bacter ial strains designated as S-1, S-2, S-3, S-4 and S-5 from paddy fields of Noakhali, Bangladesh by observing visible growth in malathion supplemented mineral salts medium (MSM) agar following selective enrichment technique. The isolates were provisionally identified based on their morphological and biochemical characteristics as Pseudomonas spp. (S-1), Bacillus subtilis (S-2), Staphylococcus aureus (S-3), Pseudomonas aeruginosa (S-4) and Pseudomonas spp. (S-5) respectively. To determine their malathion utilization potential, the isolates were inoculated in MSM containing 50 mg/l malathion as sole source of carbon. When compared with control, the turbidometric growth study with the isolates revealed that all the isolates showed a significant increase of growth, indicating utilization of malathion (conc. 50 mg/l) in MSM at 37oC. The rate of growth varied for all the isolates when this growth study was done using different temperature schemes (25oC, 35oC and 45oC). Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 17-22

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Isolation and identification of a high-efficiency phenol-degrading bacteria and optimization of its degradation conditions

E3S Web of Conferences ◽

10.1051/e3sconf/202129301028 ◽

2021 ◽

Vol 293 ◽

pp. 01028

Author(s):

Haili Sun ◽

Tianpeng Gao ◽

Guohua Chang ◽

Xisheng Tai ◽

Ruiqi Yang ◽

...

Keyword(s):

Organic Matter ◽

Sodium Chloride ◽

Low Temperature ◽

High Efficiency ◽

Phenol Degradation ◽

Pcr Amplification ◽

Toxic Substances ◽

Isolation And Identification ◽

Gram Staining ◽

Degrading Bacteria

Phenol is widely used in China, it not only pollutes the environment, but also accumulates toxic substances in the human body through the food chain, further harming humans. In this experiment, a strain of high-efficiency low-temperature degradation phenol bacteria B5 was selected from the soil contaminated by organic matter of Lanzhou. Through research methods such as Gram staining observation, DNA extraction, PCR amplification, sequencing and comparison, it was found that this strain was Pantoea agglomerans. Through the subsequent optimization of degradation conditions, it was found that the B5 strain can degrade 500mg/L of phenol to 24.8mg/L in 36h. The ability to degrade phenol is stronger between pH5.5-pH6.0, and the ability to degrade phenol is higher in a medium containing 4-8g/L sodium chloride. This research can provide certain theoretical guidance for phenol degradation.

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Characterization Of Hydrocarbon Utilizing Bacteria In Waste Engine Oil-Impacted Sites

10.1101/2020.03.21.998872 ◽

2020 ◽

Author(s):

A. M. Jones ◽

I. I. James ◽

P. S. Akpan ◽

I.I. Eka ◽

A. E. Oruk ◽

...

Keyword(s):

Micrococcus Luteus ◽

Bacterial Species ◽

Engine Oil ◽

Polluted Soils ◽

Selective Enrichment ◽

Waste Engine Oil ◽

Enrichment Technique ◽

Hydrocarbon Utilizing Bacteria ◽

Flavobacterium Aquatile

ABSTRACTThe present work was aimed at isolating and identifying hydrocarbon utilizing bacteria from waste engine oil polluted soil, and assessing their hydrocarbon-utilizing ability. Materials and method: Eight bacterial species; Corynebacterium kutscheri, Pseudomonas aeruginosa, Flavobacterium aquatile, Serratia odorifera, Micrococcus agilis, Staphylococcus aureus, Micrococcus luteus and Bacillus substilis were isolated by selective enrichment technique and screened for hydrocarbon utilization capability in mineral salt media with waste engine oil as a sole carbon and energy source. Bacterial species showed varied hydrocarbon utilization during the 15 days of incubation. All isolates also showed variable emulsification ability. These results demonstrate the presence of indigenous bacteria in hydrocarbon polluted soils and the potential towards remediation of hydrocarbons.

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Isolation and Identification of Oil Degrading Bacteria From Oil Contaminated Soil and Comparison of Their Bioremediation Potential

Global Journal For Research Analysis ◽

10.15373/22778160/august2014/61 ◽

2012 ◽

Vol 3 (8) ◽

pp. 181-184

Author(s):

Chithra. S Chithra. S ◽

◽

Hema Shenpagam. N

Keyword(s):

Contaminated Soil ◽

Isolation And Identification ◽

Degrading Bacteria

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Isolation and identification of novel geosmin-degrading bacteria

Frontiers in Bioscience ◽

10.2741/e290 ◽

2011 ◽

Vol E3 (3) ◽

pp. 830-833

Author(s):

Kazuya Shimizu

Keyword(s):

Isolation And Identification ◽

Degrading Bacteria

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Molecular Detection of Bacterial Pathogens Directly from the Nasal Swabs and Lung Tissue of Sheep and Goats

THE INDIAN JOURNAL OF VETERINARY SCIENCES AND BIOTECHNOLOGY ◽

10.21887/ijvsbt.15.2.6 ◽

2019 ◽

Vol 15 (02) ◽

pp. 22-25

Author(s):

Sunaina Thakur ◽

Subhash Verma ◽

Prasenjit Dhar ◽

Mandeep Sharma

Keyword(s):

Pasteurella Multocida ◽

Direct Detection ◽

Bacterial Species ◽

Economic Losses ◽

Isolation And Identification ◽

Sheep And Goats ◽

Histophilus Somni ◽

Nasal Swabs ◽

Mycoplasma Spp

Respiratory infections of sheep and goats cause heavy morbidity and mortality, leading to huge economic losses. Conventional methods of diagnosis that include isolation and identification of incriminating microbes are time-consuming and fraught with logistic challenges. Direct detection of incriminating microbes using molecular tools is gaining popularity in clinical, microbiological settings. In this study, a total of 50 samples (44 nasal swabs and 6 lung tissues) from sheep and goats were screened for the detection of different bacterial species by in vitro amplification of genus or species-specific genes. Histophilus somni was detected in 2% goat samples, Trueperella pyogenes in 20% goat nasal swabs, whereas 22% goat nasal swab samples were found positive for Mycoplasma spp. None of the samples from sheep was detected positive for H. somni, T. pyogenes, Mycoplasma spp. Similarly, all samples, irrespective, whether from sheep or goats, showed negative results for Pasteurella multocida, Mannheimia haemolytica, and Corynebacterium pseudotuberculosis.

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