scholarly journals Biological control of the spotted stem borer Chilo partellus (Swinhoe) (Lepidoptera: Crambidae) with the entomopathogenic fungi, Beauveria bassiana and Metarhizium anisopliae

2008 ◽  
Vol 30 (1) ◽  
Author(s):  
T Tefera ◽  
K L Pringle
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Chilo Partellus ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Stem Borer ◽  
Spotted Stem Borer

scholarly journals Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia

2018 ◽  
Vol 19 (6) ◽  
pp. 2365-2373 ◽  
Author(s):  
AYU SAFITRI ◽  
SITI HERLINDA ◽  
ARUM SETIAWAN
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Insect Pests ◽  
Vegetation Type ◽  
Tenebrio Molitor ◽  
Soil Samples ◽  
Fungal Isolates

Safitri A, Herlinda S, Setiawan A. 2018. Entomopathogenic fungi of soils of freshwater swamps, tidal lowlands, peatlands, and highlands of South Sumatra, Indonesia. Biodiversitas 19: 2365-2373. Ecosystems of lowlands and highlands in South Sumatra have specific characteristics of soils and vegetations that can affect the availability of entomopathogenic fungi. This study aimed to explore and identify species and to determine inoculum potentials of the entomopathogenic fungi from soils of freshwater swamps, tidal lowlands, peatlands, and highlands. Baiting of entomopathogenic fungi on soil samples used the larvae of Tenebrio molitor. The entomopathogenic fungi species found in this research were Beauveria bassiana and Metarhizium anisopliae. The number of the fungal isolates were 30 isolates consisting of nine isolates of B. bassiana and 21 isolates of M. anisopliae.The highest number of isolates was found in the highland ecosystem (11 isolates) and the lowest was found in peatland ecosystem (4 isolates). The highest percentage average of inoculum potentials of the fungi was found in the high land ecosystem (4.04%) and the lowest one was found in freshwater swamps ecosystem (2.11%). Based on the vegetation type, the soil planted with mustard in Talang Patai-Pagaralam (highland ecosystem) had the highest inoculum potentials (9.33%). These fungi will make an important contribution to the biological control for insect pests in lowland to highland ecosystems in Indonesia.

scholarly journals Selection of Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. strains for control of Cornitermes cumulans (Kollar)

2000 ◽  
Vol 43 (4) ◽  
pp. 373-378 ◽  
Author(s):  
Pedro J. Neves ◽  
Sérgio Batista Alves
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Production Test ◽  
Second Phase ◽  
Control Programs ◽  
Insect Mortality ◽  
Rice Medium ◽  
Selection Of

Fifty strains of the entomopathogenic fungi Beauveria bassiana (Bals.) Vuill. and Metarhizium anisopliae (Metsch.) Sorok. were tested against the termite Cornitermes cumulans. In the first phase of the experiments, several bioassays were conducted and the five best strains were selected. The criterion for strain selection during this phase was the confirmed mortality above 50% five days after application of the fungus. Three M. anisopliae and two B. bassiana strains were the most virulent. The second phase of the experiments consisted of a bioassay and a conidial production test using a rice medium. The best M. anisopliae and B. bassiana strains were selected using both the confirmed insect mortality nine days after application of the fungus and the yield of conidia. Considering results from the bioassay, M. anisopliae 1037 showed the highest confirmed (57.8%) and total (89.2%) mortalities after nine days. Among the B. bassiana strains, 447 was the most virulent with confirmed and total mortalities of 45.9% and 89.8%, respectively. M. anisopliae 1037 had the highest conidial yield with mean of 3.37 x 10(12) conidia/kg of rice, followed by B. bassiana strain 447 with 2.66 x 10(12) conidia/kg of rice. The M. anisopliae strain 1037 was the highest virulent followed by B. bassiana strain 447. This strains showed the greatest potential as agents to be used in biological control programs against C. cumulans.

scholarly journals Pathogenicity of indigenous strains of three entomopathogenic fungi to the sisal weevil, Scyphophorus acupunctatus (Gyllenhal) (Coleoptera: Curculionidae)

2015 ◽  
Vol 8 (2) ◽  
pp. 46-54
Author(s):  
V.T. Gkounti ◽  
D. Markoyiannaki ◽  
D.Ch. Kontodimas
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Fungal Species ◽  
Life Stages ◽  
Biological Control Agents ◽  
Isaria Fumosorosea ◽  
Mean Survival Time ◽  
High Pathogenicity

SummaryThe pathogenicity of indigenous isolates of Beauveria bassiana, Metarhizium anisopliae and Isaria fumosorosea was evaluated in the laboratory against larvae and adults of the sisal weevil, Scyphophorus acupunctatus. Inoculation was achieved via immersion of individuals into conidia suspensions of different concentrations. All three fungal species proved high pathogenicity against larvae of the weevil, causing 100% mortality in most of the treatments. Beauveria bassiana caused the highest mortality of the adults (86.67±12%), followed by M. anisopliae (46.67±17.8%) and I. fumosorosea (40±17.5%). Mean survival time also differed significantly among treatments and life stages of the weevil. In total, larvae survived significantly fewer days than adults post infection. Results of the present study indicate the potential of indigenous strains of entomopathogenic fungi as biological control agents against the invasive weevil.

scholarly journals SELEÇÃO DE ISOLADOS DE FUNGOS ENTOMOPATOGÊNICOS PARA O CONTROLE DE Hedypathes betulinus E AVALIAÇÃO DA PERSISTÊNCIA

FLORESTA ◽  
2011 ◽  
Vol 41 (3) ◽  
Author(s):  
Maria Sílvia Pereira Leite ◽  
Edson Tadeu Iede ◽  
Susete Do Rocio Chiarello Penteado ◽  
Scheila Ribeiro Messa Zaleski ◽  
Joelma Melissa Malherbe Camargo ◽  
...  
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Control Plot ◽  
Ilex Paraguariensis ◽  
Conidial Suspension

Hedypathes betulinus (KLUG, 1825) (Coleoptera: Cerambycidae) é a principal praga da cultura da erva-mate. Para o seu controle, avaliou-se em laboratório e campo a utilização de fungos entomopatogênicos. Em laboratório, foi avaliada a infectividade de isolados dos fungos Beauveria bassiana (Bals.) Vuillemin, 1912; B. brongniartii (Sacc.) Petch, 1926; Metarhizium anisopliae (Metsch.) Sorokin, 1883, e Paecilomyces (=Isaria) sp. Bainier, 1907, em adultos de H. betulinus. Os insetos foram imersos nas suspensões fúngicas na concentração de 107 conídios/mL. Após a aplicação, eles foram mantidos em laboratório para registro da mortalidade. Verificou-se que B. bassiana (CG 716) foi o mais infectivo, com 100% de mortalidade. Foi estimada a CL50 do isolado CG 716 de B. bassiana com cinco concentrações, variando de l05 a l09 conídios/mL, mais a testemunha. A CL50 estimada foi de 2,0x106 conídios/mL, variando de 6x105 a 5x106 conídios/mL. Foi avaliada a persistência do isolado CG 716 de B. bassiana formulado à base de óleo emulsionável em campo, verificando-se alta mortalidade nas primeiras três semanas após a aplicação do fungo, variando de 95 a 78%, decaindo para 65 a 47%, um e dois meses após a aplicação, respectivamente.Palavras-chave: Controle biológico; broca-da-erva-mate; CL50. AbstractEntomopathogenic fungi in the control of Hedypathes betulinus and evaluation of persistence. Hedypathes betulinus (KLUG, 1825) (Coleoptera: Cerambycidae) is the main pest of “erva-mate” (Ilex paraguariensis) culture. Focusing its control, it was evaluated in laboratory and field the entomopathogenic fungi using. In laboratory, it was evaluated the infectiveness of the strains of the following fungi: Beauveria bassiana (Bals.)  Vuillemin, B. brongniartii (Sacc.) Petch, Metarhizium anisopliae (Metsch.), Sorokin and Paecilomyces (= Isaria) sp. Bainier, in adults specimen of H. betulinus. The insects were immersed in a conidial suspension at a concentration of 107 conidia mL-1. After the application the insects had been kept at laboratory for mortality register. It was verified that B. bassiana (CG 716) was more infective, reaching to 100% of mortality. It estimated LC50 of isolated CG 716 of B. bassiana with five concentrations and a variation of l05 to l09 conidia mL-1, along the control plot. The estimated LC50 was 2,0 x 106 conidia mL-1, ranging from 6 x 105 to 5 x 106 conidia mL-1. The persistence of strain CG 716 of B. bassiana - formulate oil was evaluated in field, producing the highest mortality, at the first three weeks after the application of the fungus, with a variance of 95 to 78 %, with a decreasing of 65 to 47% , along one and two months after the application , respectively.Keywords: Biological control; erva-mate pest; LC50,.

scholarly journals Control biológico de Rhicephalus (Boophilus) microplus con hongos entomopatógenos / Biological Control of Rhicephalus (Boophilus) microplus with Entomopathogenic Fungi

2017 ◽  
Vol 6 (12) ◽  
pp. 33-62
Author(s):  
Arely Bautista Gálvez ◽  
Rafael Pimentel Segura ◽  
Armando Gómez-Vázquez
Keyword(s):  
Biological Control ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Boophilus Microplus

En México el problema principal de la ganadería bovina, en lo particular en el trópico, es controlar las garrapatas Rhicephalus (Boophilus) microplus, ya que el uso inadecuado de los tratamientos químicos ha creado resistencia en esos parásitos. El control biológico en garrapatas es un concepto nuevo y ha sido una medida efectiva, que ha permitido mantener estas dentro de parámetros económicos y zoosanitarios aceptables. Una de las posibilidades reales en este campo para controlar garrapatas Rhicephalus (Boophilus) microplus de importancia pecuaria es el uso de hongos entomopatógenos como el Metharrizium anisopliae y Beauveria bassiana. La finalidad del presente estudio fue observar la capacidad patógena de dos cepas de Metarhizium anisopliae a dosis de 1X108 conidias/ml y 1.3X1012 conidias/ml, y de una cepa de Beauveria bassiana a una dosis de 1.3X1012 conidias/ml en unidades de producción en bovinos de doble propósito, en los municipios de Emiliano Zapata Tabasco y la Cuenca Lechera de Catazajá, Chiapas, México; directamente en campo. Se presentó una respuesta binomial negativa de mayor porcentaje de patogenicidad en las dosis de 1.3X1012 conidias/ml de Beauveria bassiana con 76.66% a los 37.3 días de haber sido inoculada la garrapata, en contraste con las dos cepas de Metarhizium anisopliae, que mostraron una patogenicidad de 47.71% a los 10 días con la dosis 1X108 conidias/ml y de 37.75% a los 44.5 días con la dosis 1.3X1012 conidias/ml. Se presentó interacción entre tratamiento y tiempo. Por tanto, se concluye que el uso de los hongos entomopatógenos son una alternativa para el control de garrapatas adultas en el sureste de México.

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