scholarly journals Comparative assessment of poly (D,L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption

2017 ◽  
Vol 16 (10) ◽  
pp. 2315-2324
Author(s):  
Abd Almonem Doolaanea ◽  
Nur ‘Izzati Mansor ◽  
Nurul Hafizah Mohd Nor ◽  
Mohd Affendi Bin Mohd Shafri ◽  
Susi Sukmasari ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Plga Nanoparticles ◽  
Cell Uptake ◽  
Diffusion Method ◽  
Ammonium Bromide ◽  
Scanning Calorimetry ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Dna Adsorption ◽  
Cetyltrimethyl Ammonium ◽  
Positively Charged

Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption.Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight chitosan. The nanoparticles were  characterised by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and  transfection.Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1 surface. This resulted in negative zeta potential, increased  cytotoxicity as well as decreased cell uptake and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand, chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA, leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and transfection. Amongst the four formulations, F4 exhibited the highest transfection.Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB and chitosan. Modification with chitosan yields PLGA  nanoparticles with higher DNA adsorption and transfection with lower cytotoxicity.Keywords: Chitosan, cetyltrimethyl ammonium bromide (CTAB), Nanoparticle, Poly (D,L-lactide-coglycolide) PLGA, Plasmid DNA adsorption, Gene therapy

scholarly journals Preparation and thermal properties of chitosan/bentonite composite beads

2014 ◽  
Vol 68 (6) ◽  
pp. 653-659 ◽  
Author(s):  
Vesna Teofilovic ◽  
Jelena Pavlicevic ◽  
Oskar Bera ◽  
Mirjana Jovicic ◽  
Jaroslava Budinski-Simendic ◽  
...  
Keyword(s):  
Differential Scanning Calorimetry ◽  
Thermal Properties ◽  
Solution Process ◽  
Preparation Procedure ◽  
Ammonium Bromide ◽  
Scanning Calorimetry ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Composite Beads ◽  
Biopolymer Composites ◽  
Cetyltrimethyl Ammonium

Due to their biodegradable and nontoxic nature, biopolymer composites are often used as remarkable adsorbents in treatment of wastewater. In this study chitosan/bentonite composite beads were obtained by addition of clay into the polymer using solution process. Before the composite preparation, bentonite was modified with surfactant cetyltrimethyl ammonium bromide (CTAB). The morphology of beads was examined by scanning electron microscopy (SEM). Thermal properties of the composite beads were studied by simultaneous thermogravimetry coupled with differential scanning calorimetry (SDT) and differential scanning calorimetry (DSC). TG results showed that the complex decomposition mechanism of the composites depends on the preparation procedure. It was observed that the concentration of NaOH used for composites precipitation affects the final structure of beads. The influence of preparation procedure on the glass transition temperature Tg of chitosan/bentonite samples was not found (Tg values for all samples were about 144 ?C).

Scheelite (CaWO4)-type microphosphors: Facile synthesis, structural characterization and photoluminescence properties

2016 ◽  
Vol 30 (32n33) ◽  
pp. 1650400 ◽  
Author(s):  
Yuanyuan Han ◽  
Dan Wang ◽  
Danyang Liang ◽  
Shiqi Wang ◽  
Guoxin Lu ◽  
...  
Keyword(s):  
Luminescence Decay ◽  
Precipitation Method ◽  
Ammonium Bromide ◽  
Photoluminescence Properties ◽  
Facile Synthesis ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Broad Emission ◽  
Transition Formula ◽  
Cetyltrimethyl Ammonium ◽  
Uv Excitation

Scheelite (CaWO4)-type microphosphors were synthesized by the precipitation method assisted with cetyltrimethyl ammonium bromide (CTAB). All compounds crystallized in the tetragonal structure with space group [Formula: see text] (No. 88). FE-SEM micrographs illustrate the spherical-like morphologies and rough surface. PL spectra indicate the broad emission peak maximum at 613 nm under UV excitation. Luminescence decay curves monitored by [Formula: see text] transition ([Formula: see text] nm) of Eu[Formula: see text] in doped CaWO4 are presented, the curves exhibit a single-exponential feature and the lifetime for doped CaWO4 is 0.61 ms.

Fe(iii) induced structural, optical, and dielectric behavior of cetyltrimethyl ammonium bromide stabilized strontium stannate nanoparticles synthesized by a facile wet chemistry route

RSC Advances ◽  
2015 ◽  
Vol 5 (22) ◽  
pp. 17202-17209 ◽  
Author(s):  
Astakala Anil Kumar ◽  
Ashok Kumar ◽  
J. K. Quamara ◽  
G. R. Dillip ◽  
Sang Woo Joo ◽  
...  
Keyword(s):  
Dielectric Behavior ◽  
Ammonium Bromide ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Wet Chemistry ◽  
Strontium Stannate ◽  
Cetyltrimethyl Ammonium

Correlation between the structural, optical and dielectric behavior of Fe doped perovskite strontium stannate nanoparticles synthesized by a facile wet chemistry route.

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