Adaptive Mechanisms of a Mouse Locomotor Muscles "M. Soleus" and "M. Edl" the Conditions of the Allergic Modification of the Organism

International Journal of Biology and Biomedical Engineering ◽

10.46300/91011.2020.14.21 ◽

2020 ◽

Vol 14 ◽

Keyword(s):

Skeletal Muscles ◽

Slow Muscle ◽

Muscular System ◽

Compensatory Mechanisms ◽

Adaptive Mechanisms ◽

Locomotor Muscles ◽

Adaptation Processes ◽

Fast Muscles

The relevance of the problem discussed in the article is connected to the fact that mandatory athletes’ vaccination before competitions leads to the change in the function of the muscular system, the mechanisms of which have not yet been fully clarified. The purpose of the article is to determine the mechanism of a mouse skeletal muscles adaptation (SM) ("fast" (in case of m.edl) and "slow" (in case of m.soleus) in case of allergic alteration. The following research methods were used in the presented work: registration of the constrictive function of the abovementioned muscles in vitro to the humoral constriction initiators (carbacholinum and KCI) and determination of malonyldialdehyde (MDA) level in them, just as the indicators of the oxidant and antioxidant equilibrium. It has been demonstrated that the change in the “slow” muscle strength correlates with the MDA level dynamics, evidently, reflects the adaptation processes during the allergic modification. "Fast" muscles turn out to be more sustainable to oxidative stress which is most probably achieved by the work of compensatory mechanisms and is expressed in quite minor changes in the MDA dynamics. The article can be used in the search of the new possibilities for the correction of the locomotor muscles function in the conditions of the allergy, аnd also while the therapeutic impact strategy is determined, taking into account their fiber composition.

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Mechanisms of adaptation of motor «fast» and «slow» locomotor muscles in mice under conditions of allergic reorganization of the body

Spravočnik vrača obŝej praktiki (Journal of Family Medicine) ◽

10.33920/med-10-2103-07 ◽

2021 ◽

pp. 64-72

Author(s):

Aleksandr Yurievich Teplov

Keyword(s):

Sports Medicine ◽

Contractile Function ◽

The State ◽

The Body ◽

Mechanisms Of Adaptation ◽

Locomotor Muscles ◽

Action Taking ◽

Mandatory Vaccination

The state of the contractile function of transversely striated locomotor muscles under conditions of allergic reorganization remains relevant for modern sports medicine. In particular, it is known that the mandatory vaccination of athletes before the competition leads to changes in the state of the muscular system. The mechanisms of these changes are not fully understood. In the presented work, the following research methods were used: the registration of: a) the contractile function of various locomotor «fast» and «slow» muscles of the mouse in vitro on humoral initiators of contraction — carbacholine (CCh) and potassium chloride (KCI); b) indicators of the systems of pro- and antioxidant balance in the blood and in the tissues of these muscles and the determination of the level of malondialdehyde (MDA) in them. The differences in the ratios of the dynamics of the contraction force and the MDA level in both muscles demonstrate the degree of their resistance to oxidative stress, which determines the differences in the mechanisms of their adaptation to allergic reorganization. The materials of the article can be used to correct the function of locomotor muscles during vaccination, as well as to determine the strategy of therapeutic action, taking into account their fiber composition. The purpose of the article is to determine the mechanisms of adaptation of skeletal muscles (SM) of the mouse («fast» (m.EDL) and «slow» (m.Soleus)) during allergic reorganization.

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Selective LC determination of cabergoline in the bulk drug and in tablets: In vitro dissolution studies

Chromatographia ◽

10.1016/s0009-5893(07)82061-3 ◽

2007 ◽

Vol 65 (9-10) ◽

pp. 561-567

Author(s):

A ONAL ◽

O SAGIRLI ◽

D SENSOY

Keyword(s):

In Vitro Dissolution ◽

Dissolution Studies ◽

Bulk Drug

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In Vitro Validation of an Automated Method Based on Color Doppler Imaging for Determination of Flow Volume in the Presence of Nonflat Velocity Distributions

Journal of the American College of Cardiology ◽

10.1016/s0735-1097(97)88255-1 ◽

1998 ◽

Vol 31 (2) ◽

pp. 518A ◽

Cited By ~ 1

Author(s):

K Dennig

Keyword(s):

Color Doppler ◽

Color Doppler Imaging ◽

Doppler Imaging ◽

Flow Volume ◽

Automated Method

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Determination of in vitro „anti-aging„-effects of Ganoderma pfeifferi

Planta Medica ◽

10.1055/s-0030-1264580 ◽

2010 ◽

Vol 76 (12) ◽

Cited By ~ 1

Author(s):

W Jülich ◽

J Pörksen ◽

H Welzel ◽

U Lindequist

Keyword(s):

Aging Effects

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In vitro determination of the skin anti-aging potential of eleven South African plants

Planta Medica ◽

10.1055/s-0033-1352005 ◽

2013 ◽

Vol 79 (13) ◽

Author(s):

GN Ndlovu ◽

G Fouche ◽

W Cordier ◽

V Steenkamp ◽

M Tselanyane

Keyword(s):

South African ◽

South African Plants ◽

African Plants

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A Molecular Approach to Immunoscintigraphy: A Study of the T-Antigen Conformation on the Surface of Tumors

Nuklearmedizin ◽

10.1055/s-0038-1624353 ◽

1987 ◽

Vol 26 (01) ◽

pp. 1-6 ◽

Cited By ~ 2

Author(s):

S. Selvaraj ◽

M. R. Suresh ◽

G. McLean ◽

D. Willans ◽

C. Turner ◽

...

Keyword(s):

Monoclonal Antibodies ◽

Tumor Cell ◽

T Antigen ◽

Human Carcinomas ◽

Animal Tumor ◽

Synthetic Antigens

The role of glycoconjugates in tumor cell differentiation has been well documented. We have examined the expression of the two anomers of the Thomsen-Friedenreich antigen on the surface of human, canine and murine tumor cell membranes both in vitro and in vivo. This has been accomplished through the synthesis of the disaccharide terminal residues in both a and ß configuration. Both entities were used to generate murine monoclonal antibodies which recognized the carbohydrate determinants. The determination of fine specificities of these antibodies was effected by means of cellular uptake, immunohistopathology and immunoscintigraphy. Examination of pathological specimens of human and canine tumor tissue indicated that the expressed antigen was in the β configuration. More than 89% of all human carcinomas tested expressed the antigen in the above anomeric form. The combination of synthetic antigens and monoclonal antibodies raised specifically against them provide us with invaluable tools for the study of tumor marker expression in humans and their respective animal tumor models.

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Storage of Human Blood Platelets

Thrombosis and Haemostasis ◽

10.1055/s-0038-1647709 ◽

1974 ◽

Vol 32 (02/03) ◽

pp. 405-416 ◽

Cited By ~ 3

Author(s):

M. R Hardeman ◽

Carina J L. Heynens

Keyword(s):

Storage Temperature ◽

Platelet Rich Plasma ◽

Blood Platelets ◽

Storage Period ◽

Serotonin Uptake ◽

Hypotonic Shock ◽

Glycolytic Intermediates

SummaryStorage experiments were performed at 4°, 25° and 37° C with platelet-rich plasma under sterile conditions. In some experiments also the effect of storing platelets at 4° C in whole blood was investigated.Before, during and after three days of storage, the platelets were tested at 37° C for their serotonin uptake and response to hypotonic shock. In addition some glycolytic intermediates were determined.A fair correlation was noticed between the serotonin uptake and hypotonic shock experiments. Both parameters were best maintained at 25° C. Also platelet counting, performed after the storage period, indicated 25° C as the best storage temperature. Determination of glycolytic intermediates did not justify any conclusion regarding the optimal storage temperature. Of the various anticoagulants studied, ACD and heparin gave the best results as to the serotonin uptake and hypotonic shock response, either with fresh or stored platelets. The use of EDTA resulted in the lowest activity, especially after storage.The results of these storage experiments in vitro, correspond well with those in vivo reported in the literature.

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DETERMINATION OF BLOOD CORTICOIDS USING THE IN VITRO RESIN UPTAKE OF 3H-PREDNISOLONE

Acta Endocrinologica ◽

10.1530/acta.0.0570023 ◽

1968 ◽

Vol 57 (1) ◽

pp. 23-32 ◽

Cited By ~ 2

Author(s):

Hironori Nakajima ◽

Mitsunori Murala ◽

Masumitsu Nakata ◽

Takeshi Naruse ◽

Seiji Kubo

Keyword(s):

Thyroid Function Test ◽

Normal Subjects ◽

Adrenal Function ◽

Function Test ◽

Before And After ◽

Adrenal Response ◽

Suppression Test

ABSTRACT The in vitro resin uptake of 3H-prednisolone was used for the determination of blood cortisol after addition of radioactive prednisolone followed by Amberlite CG 400 Type 1 to the test serum, and incubation of the mixture. The radioactivity of the supernatant was compared before and after the addition of the resin. The principle of this method is similar to that of the 131I-triiodothyronine resin uptake for the thyroid function test. The tests for the specificity, reproducibility and sensitivity gave satisfactory results. The mean basal value ± SD of the 3H-prednisolone resin uptake was 35.3 ± 9.2% in normal subjects, and 27.1 ± 4.8% in pregnant women. This method was valid in various adrenal function tests, i. e. the adrenal circadian rhythm, corticotrophin (ACTH) test, dexamethasone suppression test and the adrenal response to lysine-8-vasopressin. It proved to be a sensitive indicator of the adrenal function. These results suggest that this method should be useful for a routine adrenal function test.

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