scholarly journals HETEROTRANSPLANTATIONS AND GENOMIC IN CANCER RESEARCH

2021 ◽  
pp. 49-51
Keyword(s):  
Cancer Research ◽  
Nude Mouse ◽  
Hela Cells ◽  
Human Tumor ◽  
Sea Star ◽  
Axial Organ ◽  
U2os Cells ◽  
Tumoral Cells

We performed ,in 1975, the first heterotransplantation of invertebrate A.O in nude mouse, then a double heterotransplantation of human tumor and Axial organ next to this last one, always in nude mouse: The human tumor was rejected in 50% of observed cases. Some years later, we found that A.O cells exerted an induced and spontaneous cytotoxicity against SP2 and MBL2 mouse tumoral cells. Recently, we discovered a sea star Igkappa gene with immune properties. This gene was inserted in a CMV(cytomegalovirus) and finally in a plasmid called « young » plasmid. The induced« young » protein exerted a spontaneous cytotoxicity against osteosarcom cells (U2oS cells) against A-375 melanome cells and Hela cells

scholarly journals The Sea Star Igkappa Gene and Cancerology

2017 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Hela Cells ◽  
Carcinoma Cells ◽  
Cervix Carcinoma ◽  
Sea Star ◽  
Axial Organ ◽  
Cancerous Cells

It was shown 32 years ago that the sea star axial organ cells (AO cells) produced a spontaneous cytotoxicity against mouse cancerous cells. Recently, we discovered a sea star Igkappa gene with immune properties. This gene was inserted in a CMV (cytomegalovirus) and finally in a plasmid called « young » plasmid. The induced« young » protein exerted a spontaneous cytotoxicity against Hela cells (cervix carcinoma cells) and at a weaker degree against dendritic celle, MSC cells.

Synthesis & Anticancer Evaluation of New Substituted 2-(3,4- Dimethoxyphenyl)benzazoles

2019 ◽  
Vol 15 (3) ◽  
pp. 287-297 ◽  
Author(s):  
Cigdem Karaaslan ◽  
Yalcin Duydu ◽  
Aylin Ustundag ◽  
Can O. Yalcin ◽  
Banu Kaskatepe ◽  
...  
Keyword(s):  
Hela Cells ◽  
Parp Inhibitor ◽  
A549 Cells ◽  
Human Tumor ◽  
Antimicrobial Activities ◽  
Sodium Metabisulfite ◽  
Human Tumor Cell Lines ◽  
Antibacterial And Antifungal Activities ◽  
Antibacterial And Antifungal ◽  
Mitotic Inhibitor

Background: The benzazole nucleus is found in many promising small molecules such as anticancer and antibacterial agents. Bendamustine (Alkylating agent), Nocodazole (Mitotic inhibitor), Veliparib (PARP inhibitor), and Glasdegib (SMO inhibitor) are being clinically used as anticancer therapeutic which bear benzimidazole moiety. Based on the principle of bioisosterism, in the present work, 23 compounds belonging to 2-(3,4-dimethoxyphenyl)benzazoles and imidazopyridine series were synthesized and evaluated for their anticancer and antimicrobial activities. Objective: A series of new 2-(3,4-dimethoxyphenyl)-1H-benz(or pyrido)azoles were synthesized and evaluated for their anticancer and antimicrobial activities. Method: N-(5-chloro-2-hdroxyphenyl)-3,4-dimethoxybenzamide 1, was obtained by the amidation of 2-hydroxy-5-chloroaniline with 3,4-dimethoxybenzoic acid by using 1,1&'-carbonyldiimidazole. Cyclization of 1 to benzoxazole derivative 2, was achieved by p-toluenesulfonic acid. Other 1H-benz(or pyrido)azoles were prepared by the reaction between 2-aminothiophenol, ophenylenediamine, o-pyridinediamine with sodium metabisulfite adduct of 3,4-dimethoxybenzaldehyde. The NMR assignments of the dimethoxy groups were established by the NOESY spectra. Results: Compound 12, bearing two chlorine atoms at the 5(4) and 7(6) positions of the benzene moiety of benzimidazole was found the most potent analogue against A549 cells with the GI50 value of 1.5 μg/mL. Moreover, 24 showed remarkable cell growth inhibition against MCF-7 and HeLa cells with the GI50 values of 7 and 5.5 μg/mL, respectively. The synthesized compounds have no important antibacterial and antifungal activities. Conclusion: It could be concluded that the introduction of di-chloro atoms at the phenyl ring of 2-(3,4-dimethoxyphenyl)-1H-benzimidazoles increases significant cytotoxicity to selected human tumor cell lines in comparison to other all benzazoles synthesized. Unsubstituted 2-(3,4- dimethoxyphenyl)-imidazopyridines also gave good inhibitory profile against A549 and HeLa cells.

scholarly journals Spontaneous and Induced Animal Models for Cancer Research

Diagnostics ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 660
Author(s):  
Anca Onaciu ◽  
Raluca Munteanu ◽  
Vlad Cristian Munteanu ◽  
Diana Gulei ◽  
Lajos Raduly ◽  
...  
Keyword(s):  
Cancer Research ◽  
Animal Models ◽  
Mouse Models ◽  
Behavioral Science ◽  
Laboratory Mouse ◽  
Human Tumor ◽  
Large Animal ◽  
Large Animal Models ◽  
Oncology Research ◽  
Genetically Engineered Mice

Considering the complexity of the current framework in oncology, the relevance of animal models in biomedical research is critical in light of the capacity to produce valuable data with clinical translation. The laboratory mouse is the most common animal model used in cancer research due to its high adaptation to different environments, genetic variability, and physiological similarities with humans. Beginning with spontaneous mutations arising in mice colonies that allow for pursuing studies of specific pathological conditions, this area of in vivo research has significantly evolved, now capable of generating humanized mice models encompassing the human immune system in biological correlation with human tumor xenografts. Moreover, the era of genetic engineering, especially of the hijacking CRISPR/Cas9 technique, offers powerful tools in designing and developing various mouse strains. Within this article, we will cover the principal mouse models used in oncology research, beginning with behavioral science of animals vs. humans, and continuing on with genetically engineered mice, microsurgical-induced cancer models, and avatar mouse models for personalized cancer therapy. Moreover, the area of spontaneous large animal models for cancer research will be briefly presented.

scholarly journals Critical Role of PICT-1, a Tumor Suppressor Candidate, in Phosphatidylinositol 3,4,5-Trisphosphate Signals and Tumorigenic Transformation

2006 ◽  
Vol 17 (11) ◽  
pp. 4888-4895 ◽  
Author(s):  
Fumiaki Okahara ◽  
Kouichi Itoh ◽  
Akira Nakagawara ◽  
Makoto Murakami ◽  
Yasunori Kanaho ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Hela Cells ◽  
Critical Role ◽  
Human Tumor ◽  
Causative Factor ◽  
Dependent Manner ◽  
Human Neuroblastoma ◽  
Pten Protein ◽  
Nih3t3 Cells ◽  
Tumorigenic Transformation

The tumor suppressor phosphatase and tensin homolog deleted on chromosome 10 (PTEN) regulates diverse cellular functions by dephosphorylating the lipid second messenger, phosphatidylinositol 3,4,5-trisphosphate (PIP3). Recent study revealed that PICT-1/GLTSCR2 bound to and stabilized PTEN protein in cells, implicating its roles in PTEN-governed PIP3 signals. In this study, we demonstrate that RNA interference-mediated knockdown of PICT-1 in HeLa cells down-regulated endogenous PTEN and resulted in the activation of PIP3 downstream effectors, such as protein kinase B/Akt. Furthermore, the PICT-1 knockdown promoted HeLa cell proliferation; however the proliferation of PTEN-null cells was not altered by the PICT-1 knockdown, suggesting its dependency on PTEN status. In addition, apoptosis of HeLa cells induced by staurosporine or serum-depletion was alleviated by the PICT-1 knockdown in the similar PTEN-dependent manner. Most strikingly, the PICT-1 knockdown in HeLa and NIH3T3 cells promoted anchorage-independent growth, a hallmark of tumorigenic transformation. Furthermore, PICT-1 was aberrantly expressed in 18 (41%) of 44 human neuroblastoma specimens, and the PICT-1 loss was associated with reduced PTEN protein expression in spite of the existence of PTEN mRNA. Collectively, these results suggest that PICT-1 plays a role in PIP3 signals through controlling PTEN protein stability and the impairment in the PICT-1–PTEN regulatory unit may become a causative factor in human tumor(s).

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