Expanding n-stationary Metabolic Flux Analysis to Unravel the Impact of Photorespiration on Central Metabolism

2020 ◽  
Author(s):  
Xinyu Fu
Keyword(s):  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Central Metabolism ◽  
The Impact

Metabolic flux analysis gives an insight on verapamil induced changes in central metabolism of HL-1 cells

2011 ◽  
Vol 155 (3) ◽  
pp. 299-307 ◽  
Author(s):  
Alexander Strigun ◽  
Fozia Noor ◽  
Alejandro Pironti ◽  
Jens Niklas ◽  
Tae Hoon Yang ◽  
...  
Keyword(s):  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Central Metabolism ◽  
Induced Changes

A metabolic flux analysis to study the role of sucrose synthase in the regulation of the carbon partitioning in central metabolism in maize root tips

2007 ◽  
Vol 9 (5-6) ◽  
pp. 419-432 ◽  
Author(s):  
Ana Paula Alonso ◽  
Philippe Raymond ◽  
Michel Hernould ◽  
Corinne Rondeau-Mouro ◽  
Albert de Graaf ◽  
...  
Keyword(s):  
Metabolic Flux Analysis ◽  
Sucrose Synthase ◽  
Metabolic Flux ◽  
Carbon Partitioning ◽  
Maize Root ◽  
Flux Analysis ◽  
Central Metabolism ◽  
Root Tips

scholarly journals Quantifying Central Metabolic Fluxes in Human Platelets Using Metabolic Flux Analysis

2021 ◽  
Author(s):  
Cara L Sake ◽  
Alexander J Metcalf ◽  
Jorge DiPaola ◽  
Keith B Neeves ◽  
Nanette Boyle
Keyword(s):  
Metabolic Flux Analysis ◽  
Metabolic Flux ◽  
Tricarboxylic Acid Cycle ◽  
Human Platelets ◽  
Flux Analysis ◽  
Central Metabolism ◽  
Metabolic Fluxes ◽  
Activated Platelets ◽  
Flux Measurements ◽  
Reaction Level

Platelet metabolism is linked to platelet hyper- and hypoactivity in numerous human diseases. Most studies of platelet metabolism use extracellular uptake and excretion measurements or metabolomics to infer metabolism changes but have not quantified the carbon flux through central metabolism. The reaction-level resolution is necessary to identify the major contributors to different platelet phenotypes. The goal of this study was to develop the metabolic flux map of resting and agonist activated platelets based on intracellular flux measurements of central metabolism. Isotopically nonstationary 13C metabolic flux analysis (INST-MFA) was used to measure metabolic fluxes in platelets from labeling profiles obtained with parallel glucose and acetate labeling experiments. Flux results show that resting platelets primarily metabolize glucose to lactate via glycolysis, while acetate is oxidized to fuel the tricarboxylic acid cycle. Upon activation with thrombin, a potent platelet agonist, global flux increases occur, and platelets display a metabolic shift toward glucose oxidation.

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