Dietary Lipid Level Affects Growth Performance, Antioxidant Capacity, Hematological Characteristics and Lipid Metabolism in Juvenile Black Seabream (Acanthopagrus schlegelii)

AbstractThe regulation of lipogenesis and lipolysis mechanisms related to consumption of lipid has not been studied in swimming crab. The aims of the present study were to evaluate the effects of dietary lipid levels on growth, enzymes activities and expression of genes of lipid metabolism in hepatopancreas of juvenile swimming crab. Three isonitrogenous diets were formulated to contain crude lipid levels at 5·8, 9·9 and 15·1 %. Crabs fed the diet containing 15·1 % lipid had significantly lower growth performance and feed utilisation than those fed the 5·8 and 9·9 % lipid diets. Crabs fed 5·8 % lipid had lower malondialdehyde concentrations in the haemolymph and hepatopancreas than those fed the other diets. Highest glutathione peroxidase in haemolymph and superoxide dismutase in hepatopancreas were observed in crabs fed 5·8 % lipid. The lowest fatty acid synthase and glucose 6-phosphate dehydrogenase activities in hepatopancreas were observed in crabs fed 15·1 % lipid, whereas crabs fed 5·8 % lipid had lower carnitine palmitoyltransferase-1 activity than those fed the other diets. Crabs fed 15·1 % lipid showed lower hepatopancreas expression of genes involved in long-chain-PUFA biosynthesis, lipoprotein clearance, fatty acid uptake, fatty acid oxidation, lipid anabolism and lipid catabolism than those fed the other diets, whereas expression of some genes of lipoprotein assembly and fatty acid oxidation was up-regulated compared with crabs fed 5·8 % lipid. Overall, high dietary lipid level can inhibit growth, reduce antioxidant enzyme activities and influence lipid metabolic pathways to regulate lipid deposition in crab.

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The effect of dietary L-carnitine on the growth performance in fingerlings of the african catfish (Clarias gariepinus) in relation to dietary lipid

British Journal Of Nutrition ◽

10.1079/bjn19930030 ◽

1993 ◽

Vol 69 (1) ◽

pp. 289-299 ◽

Cited By ~ 56

Author(s):

Els Torreele ◽

Ad van Der Sluiszen ◽

Johan Verreth

Keyword(s):

Growth Performance ◽

Lipid Level ◽

Clarias Gariepinus ◽

Live Weight ◽

Growth Efficiency ◽

Dietary Lipid ◽

African Catfish ◽

Feed Conversion ◽

Energy Retention ◽

Dietary Lipid Level

The effect of dietary L-carnitine on the growth and growth efficiency of African catifish (Clarias gariepinus, Burchell 1822) fingerlings was investigated. Six dietary levels of L-carnitine, varying from the conrol level (about 125 mg/kg) to 3920 mg/kg, were each tested at two dietary lipid levels (96 and 155 g/kg). The diets were isonitrogenous and were fed to thirty-six experimental groups of 100 fish weighing 5 g at a feeding level of 25·2 g/kg live weight(w)0.8 per d, during 18 d. The average final weight varied from 19·1 to 28·0 g. At a dietary lipid level of 96 g/kg the metabolic growth increased from 30·8 to 36·5 g/kg w0.8 per d. At the higher dietary lipid level the metabolic growth increased from 30·9 to 35·4 g/kg w0.8 per d. To assess the dose–response relationship between dietary L-carnitine and growth performance in the African catfish a linear-plateau model was fitted to the experimental data. According to this model, metabolic growth was at a maximum at L-carnitine levels of 500 mg/kg and above at a lipid level of 96 g/kg and at L-carnitine levels of 684 mg/kg and above at a lipid level of 155 g/kg. The fitted maximum metabolic growth was higher at a dietary lipid level of 96 g/kg (35·9 g/kg w0.8 per d) than at 155 g/kg (34·7 g/kg w0.8 per d). Feed conversion improved significantly with increasing dietary levels of L-carnitine, reaching a fitted plateau at L-carnitine levels of 448·8 and 236·7 mg/kg respectively for the high and low dietary fat levels. Other growth efficiency variables, e.g. protein efficiency ratio, protein retention and energy retention improved accordingly. Taking into consideration that all fish received the same amount of feed, the results of the present study demonstrate that the positive effect of increased levels of dietary L-carnitine is the result of an improved feed utilization, probably because of a stimulated protein-sparing action.

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Effects of dietary lipid level on growth performance, body composition and digestive enzymes activity of juvenile sea cucumber,Apostichopus japonicus

Aquaculture Research ◽

10.1111/are.12864 ◽

2015 ◽

Vol 48 (1) ◽

pp. 92-101 ◽

Cited By ~ 10

Author(s):

Ming-Ling Liao ◽

Tong-Jun Ren ◽

Wei Chen ◽

Yu-Zhe Han ◽

Chen-Min Liu ◽

...

Keyword(s):

Body Composition ◽

Growth Performance ◽

Digestive Enzymes ◽

Sea Cucumber ◽

Apostichopus Japonicus ◽

Lipid Level ◽

Dietary Lipid ◽

Enzymes Activity ◽

Dietary Lipid Level

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Effect of dietary lipid level on growth, lipid metabolism and oxidative status of largemouth bass, Micropterus salmoides

Aquaculture ◽

10.1016/j.aquaculture.2019.04.007 ◽

2019 ◽

Vol 506 ◽

pp. 394-400 ◽

Cited By ~ 8

Author(s):

Jia-ling Guo ◽

Yue-lang Zhou ◽

Hang Zhao ◽

Wen-Yan Chen ◽

Yong-Jun Chen ◽

...

Keyword(s):

Lipid Metabolism ◽

Largemouth Bass ◽

Micropterus Salmoides ◽

Lipid Level ◽

Dietary Lipid ◽

Oxidative Status ◽

Dietary Lipid Level

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Dietary lipid and n-3 long-chain PUFA levels impact growth performance and lipid metabolism of juvenile mud crab, Scylla paramamosain

British Journal Of Nutrition ◽

10.1017/s0007114520003335 ◽

2020 ◽

pp. 1-15 ◽

Cited By ~ 1

Author(s):

Xuexi Wang ◽

Min Jin ◽

Xin Cheng ◽

Jiaxiang Luo ◽

Lefei Jiao ◽

...

Keyword(s):

Fatty Acid ◽

Lipid Metabolism ◽

Weight Gain ◽

Growth Performance ◽

Lipid Level ◽

Dietary Lipid ◽

Scylla Paramamosain ◽

Mud Crab ◽

Lipid Levels ◽

Expression Levels

Abstract An 8-week feeding trial was conducted to evaluate the effects of dietary n-3 LC-PUFA levels on growth performance, tissue fatty acid profiles and relative expression of genes involved in the lipid metabolism of mud crab (Scylla paramamosain). Ten isonitrogenous diets were formulated to contain five n-3 LC-PUFA levels at 7 and 12 % dietary lipid levels. The highest weight gain and specific growth rate were observed in crabs fed the diets with 19·8 and 13·2 mg/g n-3 LC-PUFA at 7 and 12 % lipid, respectively. Moisture and lipid contents in hepatopancreas and muscle were significantly influenced by dietary n-3 LC-PUFA at the two lipid levels. The DHA, EPA, n-3 LC-PUFA contents and n-3:n-6 PUFA ratio in hepatopancreas and muscle significantly increased as dietary n-3 LC-PUFA levels increased at both lipid levels. The expression levels of Δ-6 fatty acyl desaturase and acyl-CoA oxidase in hepatopancreas increased significantly, and expression levels of fatty acid synthase, carnitine palmitoyltransferase I and hormone-sensitive TAG lipase were down-regulated, with increased dietary n-3 LC-PUFA regardless of lipid level. Based on weight gain, n-3 LC-PUFA requirements of S. paramamosain were estimated to be 20·1 and 12·7 mg/g of diet at 7 and 12 % dietary lipid, respectively. Overall, dietary lipid level influenced lipid metabolism, and purified, high-lipid diets rich in palmitic acid reduced the n-3 LC-PUFA requirement of juvenile mud crab.

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