IDENTIFICATION OF CYTOGENETICALLY VISIBLE mtDNA IN INTERPHASE NUCLEI OF HUMAN BLOOD LYMPHOCYTES EXPOSED TO DOXORUBICIN

2020 ◽  
Vol 54 (1 (251)) ◽  
pp. 63-67
Author(s):  
T.A. Harutyunyan ◽  
G.G. Hovhannisyan ◽  
A.A. Sargsyan ◽  
T. Liehr ◽  
A. Al-Rikabi ◽  
...  
Keyword(s):  
Human Blood ◽  
Dna Translocation ◽  
Interphase Nuclei ◽  
Free Living ◽  
Blood Lymphocytes ◽  
Dna Damaging Agents ◽  
Human Blood Lymphocytes ◽  
Living Organisms ◽  
The Impact

The endosymbiotic hypothesis suggests that mitochondria were free-living organisms that colonized another cells, and millions of years of co-evolution resulted in the transfer of a large number of genes from mitochondria to the nucleus. Recent studies indicate that this is an ongoing process and can be due to the impact of cells to DNA damaging agents, e.g. radiation or chemical mutagens. Here we demonstrate that cytogenetically visible mitochondrial DNA translocation into interphase nuclei genome in human blood lymphocytes can be identified via fluorescence in situ hybridization after exposure of cells to doxorubicin.

Genotoxic effects of chromium(VI) and cadmium(II) in human blood lymphocytes using the electron microscopy in situ end-labeling (EM-ISEL) assay

2006 ◽  
Vol 20 (4) ◽  
pp. 513-518 ◽  
Author(s):  
François Depault ◽  
Marilena Cojocaru ◽  
Fléchère Fortin ◽  
Saroj Chakrabarti ◽  
Nicole Lemieux
Keyword(s):  
Electron Microscopy ◽  
Human Blood ◽  
Genotoxic Effects ◽  
Blood Lymphocytes ◽  
Chromium Vi ◽  
Human Blood Lymphocytes

Study of interaction between l-glutamate and human blood lymphocytes

1997 ◽  
Vol 58 (3) ◽  
pp. 177-180 ◽  
Author(s):  
I.A Kostanyan ◽  
M.I Merkulova ◽  
E.V Navolotskaya ◽  
R.I Nurieva
Keyword(s):  
Human Blood ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes

A simplified and reliable assay for complex I in human blood lymphocytes

2007 ◽  
Vol 326 (1-2) ◽  
pp. 76-82 ◽  
Author(s):  
L.E.A. de Wit ◽  
L. Spruijt ◽  
G.C. Schoonderwoerd ◽  
I.F.M. de Coo ◽  
H.J.M. Smeets ◽  
...  
Keyword(s):  
Human Blood ◽  
Complex I ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes

Modulation of UVB-induced Oxidative Stress by Ursolic Acid in Human Blood Lymphocytes

2007 ◽  
Vol 3 (1) ◽  
pp. 11-18 ◽  
Author(s):  
S. Ramachandr ◽  
N. Rajendra Prasad ◽  
K.V. Pugalendi ◽  
Venugopal P. Menon
Keyword(s):  
Oxidative Stress ◽  
Ursolic Acid ◽  
Human Blood ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes

Potential of rod, sphere and semi-cube shaped gold nanoparticles to induce cytotoxicity and genotoxicity in human blood lymphocytes in vitro

2015 ◽  
Vol 7 (1) ◽  
Author(s):  
Mona A.M. Abo-Zeid ◽  
Thomas Liehr ◽  
Amira M. Gamal-Eldeen ◽  
Mahmoud Zawrah ◽  
Mostafa Ali ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Gold Nanoparticles ◽  
Mitotic Activity ◽  
Human Blood ◽  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
Blood Lymphocytes ◽  
Human Blood Lymphocytes ◽  
Different Shapes

AbstractGold nanoparticles (GNPs) are intended to be used in nanomedicine. Due to nanotechnology innovation GNPs of variable sizes and in different shapes including rods, spheres, cubes, etc., can easily be produced. The aim of the present studies was to evaluate the cyto-and genotoxicity inducible by different shaped GNPs on normal human peripheral blood lymphocytes.Four different shapes of GNPs including big rod GNPs (BR-GNPs, 50 nm), small rod GNPs (SR-GNPs, 30 nm), sphere GNPs (S-GNPs, 15 nm) and semi-cube GNPs (SC-GNPs, 15 nm) were studied. Cultured human blood lymphocytes were treated with different concentrations of these GNPs for 24 h in vitro. Cytotoxicity was evaluated based on the mitotic index (MI), while genotoxicity was studied by an interphase-fluorescence in situ hybridization (I-FISH) assay. The following genes were studied in I-FISH:The lowest concentration of BR-GNPs neither had an effect mitotic activity nor enhanced gain or loss of examined gene signals in a significant manner with I-FSH. Other concentrations of BR-GNPs, SR-GNPs, S-GNPs and SC-GNPs with all concentrations inhibited the mitotic activity of the cells and reduced the cell proliferation highly significantly. The different types of GNPs initiated the duplication ofGNPs at high concentration can reduce the cell proliferation and induce DNA damage. Low concentration of rod-shaped GNPs at 50 nm was safe on human lymphocytes. Further research studies are required to optimize the concentration, shape and size of GNPs before using them in nanomedicine.

Different distributions of homologous chromosomes in adult human Sertoli cells and in lymphocytes signify nuclear differentiation

1996 ◽  
Vol 109 (4) ◽  
pp. 773-776 ◽  
Author(s):  
A.C. Chandley ◽  
R.M. Speed ◽  
A.R. Leitch
Keyword(s):  
Sertoli Cells ◽  
Cell Types ◽  
Fish Analysis ◽  
Cell Nuclei ◽  
Interphase Nuclei ◽  
Homologous Chromosomes ◽  
Blood Lymphocytes ◽  
Painting Probes ◽  
Whole Chromosome Painting

Using whole chromosome painting probes for human chromosomes 3,7,8,13,17 and 21 and X and the probe pHY2.1 for the Y chromosome coupled with fluorescent in situ hybridization (FISH) analysis, the distribution of chromosomes is reported in nuclei of Sertoli cells of the adult testis and in stimulated blood lymphocytes. The distribution of chromosomes in the two cell types is significantly different. A strong tendency for each pair of homologues to pair is inferred from the observation of only a single detectable signal in the majority of Sertoli cell nuclei. The sex chromosomes, by contrast, give two clearly separated signals. Interphase nuclei in dividing blood lymphocytes, analysed as controls, also show mainly two separated signals for all non-acrocentric autosomal pairs, but acrocentric pairs no. 13 and 21 show some tendency to associate, probably reflecting satellite association.

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