scholarly journals Experimental evaluation of the activity of the product meflochine against coronavirus SАRS-CoV-2

2020 ◽  
Author(s):  
KN Filin ◽  
IA Berzin ◽  
VN Bykov ◽  
VD Gladkikh ◽  
SYa Loginova ◽  
...  

When evaluating the effectiveness of the drug Mefloquine against SARS-Cov-2 coronavirus, in vitro experiments examined its toxicity for African green monkey kidney cell culture - Vero C1008, as well as antiviral activity against SARS-Cov-2, which was evaluated by suppressing the cytopathic effect the virus. A study of the toxicity of the drug Mefloquine showed that the concentration at which the drug exerts a cytopathic effect against 50% of Vero C1008 cells is 4.5 μg / ml. The maximum tolerated concentration (MTD) of Mefloquine is 2.25 μg / ml. A study of the effectiveness showed that 1 day after infection, the antiviral effect of Mefloquine was recorded when the drug was added 24 hours and 1 hour before infection with SARS-CoV-2, as well as when it was added 1 hour after infection, the cell culture was already at a concentration of 0.5 μg / ml Mefloquine at a concentration of 2 μg / ml, added to the Vero C1008 cell culture 1 hour after the introduction of SARS-CoV-2, completely blocked the action of the virus for 2 days after infection.

1969 ◽  
Vol 15 (5) ◽  
pp. 451-454 ◽  
Author(s):  
J. Joncas ◽  
A. Chagnon ◽  
G. Lussier ◽  
V. Pavilanis

A human fetal lung cell culture contaminant possessing most of the characteristics of that described by Girardi et al. (5) was shown to belong to the M. hyorhinis-GDL group of cytopathic mycoplasma by accessory factor neutralization test. The homotypic antiserum in this test neutralized the contaminant as well as three closely related strains while it could inhibit the growth of the contaminant only on agar. The mycoplasma could be detected in tissue cultures by the acridine orange staining technique and by direct immunofluorescence. These appeared as minute coccobacillary bodies either isolated or in clumps, predominantly extracellular in the original tissue culture but apparently intracellular in monkey kidney cell culture. A titer of 106 TCID50 per 0.1 ml was obtained in monkey kidney cell culture and the cytopathic effect induced in this culture was inhibited by pretreatment of the agent with 20% ether for 18 hours at 4 C, with tris buffer at pH 3 for 3 hours, or by heating at 56 C for 3 minutes. The cytopathic effect was suppressed by chlortetracycline (50 μg/ml) and neutralized with accessory factor by specific antiserum produced in rabbit up to a titer of 8192, whereas a variety of normal human and animal sera failed to do so. The source of the contaminant could not be determined.


Sign in / Sign up

Export Citation Format

Share Document