scholarly journals Viral complexes in the Allium cepa L.- Frankliniella occidentalis P. interaction by DAS-ELISA in Baja California, Mexico

2021 ◽  
Vol 38 (3) ◽  
pp. 585-607
Keyword(s):  
Allium Cepa ◽  
Frankliniella Occidentalis ◽  
Baja California ◽  
Tomato Spot Wilt Virus ◽  
Yellow Spot ◽  
Onion Yellow Dwarf Virus ◽  
Spot Virus ◽  
Indicator Plants ◽  
Allium Cepa L ◽  
Yellow Dwarf Virus

The onion (Allium cepa L.) is one of the main horticultural crops produced in Baja California, Mexico. In this crop, it has been observed presumptive viral symptoms, which are able to cause losses in yield and quality. Therefore, the objective of this study was to detect the associated virus in onion in high producing culture zones in Baja California. Plant material samples (symptomatic and asymptomatic) and trips insects were collected from commercial farming or areas. Commercial anti-serum were used for the detection of Iris yellow spot virus (IYSV), Tomato spot wilt virus (TSWV), Leek yellow spot virus (LYSV), Onion yellow dwarf virus (OYDV) and Garlic common latent virus (GarCLV). Seven indicator plants species were mechanically inoculated with onion sap (symptomatic and asymptomatic). The results showed the presence of IYSV, TSWV, OYDV and GarCLV in symptomatic onion plants. The plant samples showed a 77 % of incidence in the form of viral complexes and in a 23 % only the presence of LYSV was detected. The trips identified as Frankiniella occidentalis P. harbored IYSV, TSWV, LYSV, OYDV and GarCLV in the form of complexes in La Trinidad and San Quintin. The indicator plants did not show sympotoms of virosis. It is evident that F. occidentalis P. is responsible for the transmision of the analyzed viruses in this study in cultured onions. There are not previous reports for the detection of Orthotospovirus, Potyvirus and Carlavirus in Baja California onions.

scholarly journals Variabilidad y síntomas causados por Iris yellow spot virus en Nicotiana benthamiana

2017 ◽  
Vol 35 (4) ◽  
Author(s):  
Katya Ornelas-Ocampo ◽  
Daniel Leobardo Ochoa-Martínez ◽  
Sergio Aranda-Ocampo ◽  
Sergio Ramírez-Rojas ◽  
Hernán García-Ruíz
Keyword(s):  
Allium Cepa ◽  
Nicotiana Benthamiana ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus

<p>En cultivos de cebolla <em>Allium cepa</em> del estado de Morelos, México, se observan síntomas típicos y severos asociados a <em>Iris yellow spot virus </em>(IYSV). En esta investigación se estudiaron las alteraciones que ocasionan los aislamientos de IYSV procedentes de síntomas típicos y severos en <em>Nicotiana benthamiana</em>, las diferencias en el gen N y su filogenia. Cuatro aislamientos típicos y cinco severos inoculados mecánicamente causaron infección sistémica. En cámara bioclimática los aislamientos severos ocasionaron mayor severidad de síntomas. La secuencia del gen N de ambos aislamientos tuvo 98-99% de identidad con la nucleoproteína de IYSV y no se observaron cambios en la secuencia de nucleótidos entre ellos. Ambos aislamientos se agruparon con el genotipo IYSV<sub>BR</sub> y tuvieron mayor similitud con los reportados en Canadá y Estados Unidos.</p>

scholarly journals Distribution and Transmission of Iris yellow spot virus

Plant Disease ◽  
2001 ◽  
Vol 85 (8) ◽  
pp. 838-842 ◽  
Author(s):  
A. Kritzman ◽  
M. Lampel ◽  
B. Raccah ◽  
A. Gera
Keyword(s):  
Frankliniella Occidentalis ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Onion Thrips ◽  
Spot Virus ◽  
Nucleocapsid Gene ◽  
Thrips Species ◽  
Mother Plants ◽  
Thrips Population

Iris yellow spot virus (IYSV), a new tospovirus associated with a disease in onion (Allium cepa) that is known to growers in Israel as “straw bleaching,” was identified and further characterized by host range, serology, electron microscopy, and molecular analysis of the nucleocapsid gene. The transmissibility of IYSV by Thrips tabaci and Frankliniella occidentalis was studied. IYSV was efficiently transmitted by T. tabaci from infected to healthy onion seedlings and leaf pieces. Two biotypes of F. occidentalis, collected from two different locations in Israel, failed to transmit the virus. Surveys to relate the incidence of thrips populations to that of IYSV were conducted in onion fields. They revealed that the onion thrips T. tabaci was the predominant thrips species, and that its incidence was strongly related to that of IYSV. Forty-five percent of the thrips population collected from IYSV-infected onion and garlic fields in Israel transmitted the virus. IYSV was not transmitted to onion seedlings from infected mother plants through the seed, and was not located in bulbs of infected plants.

scholarly journals Susceptibility of Onion Relatives (Allium spp.) to Iris yellow spot virus

Plant Disease ◽  
2011 ◽  
Vol 95 (10) ◽  
pp. 1319-1319 ◽  
Author(s):  
C. S. Cramer ◽  
S. Bag ◽  
H. F. Schwartz ◽  
H. R. Pappu
Keyword(s):  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
N Gene ◽  
Rt Pcr ◽  
Sources Of Resistance ◽  
Spot Virus ◽  
Allium Species ◽  
Elisa Kit ◽  
Important Constraint ◽  
Allium Cepa L

Iris yellow spot virus (IYSV; family Bunyaviridae, genus Tospovirus) is becoming an increasingly important constraint to the production of bulb and seed onions (Allium cepa L.) in many onion-growing regions of the continental United States and the world (4). During an evaluation of onion germplasm for susceptibility to IYSV, six other Allium species (A. altaicum, A. galanthum, A. roylei, A. schoenoprasum, A. tuberosum, and A. vavilovii) were also evaluated under natural field conditions. In July 2010, symptoms suggestive of IYSV infection (straw-colored necrotic lesions) were observed on leaves of these Allium spp. in experimental plots in Las Cruces, NM. IYSV was detected in symptomatic leaves of A. altaicum, A. vavilovii, A. tuberosum, A. schoenoprasum and A. roylei with a commercially available ELISA kit (Agdia Inc., Elkhart, IN). IYSV infection was confirmed by reverse transcription (RT)-PCR with forward and complementary primers 5′-CTCTTAAACACATTTAACAAGCAC-3′ and 5′-TAAAACAAACATTCAAACAA-3′ flanking the nucleocapsid (N) gene encoded by the small RNA of IYSV as previously described (1,3). Amplicons, approximately 1.1 kb long, were obtained from all symptomatic Allium spp. samples but not from healthy samples or water controls. Sequencing of selected amplicons confirmed IYSV infection. The highest nucleotide identity of 98% was shared with IYSV isolates from Japan (GenBank Accession No. AB180921). A. altaicum, A. vavilovii, and A. pskemense were previously reported from Washington to be susceptible to IYSV (2). Current findings expand the list of Allium spp. that are susceptible to IYSV and underscores the need for continued screening of other members of the genus to find sources of resistance to IYSV. References: (1) H. R. Pappu et al. Arch. Virol. 151:1015, 2006. (2) H. R. Pappu et al. Plant Dis. 90:378, 2006. (3) H. R. Pappu et al. Plant Dis. 92:588, 2008. (4) H. R. Pappu et al. Virus Res. 141:219, 2009.

scholarly journals First Report of Onion Yellow Dwarf Virus on Shallot (Allium cepa var. aggregatum) in China

Plant Disease ◽  
2019 ◽  
Vol 103 (4) ◽  
pp. 778-778 ◽  
Author(s):  
Y. Su ◽  
L. X. Liu ◽  
B. Liu ◽  
Y. Su ◽  
Y. Z. Wang ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Yellow Dwarf ◽  
Dwarf Virus ◽  
Onion Yellow Dwarf Virus ◽  
First Report ◽  
Yellow Dwarf Virus

scholarly journals Potential factors contributing to the decline of Iris Yellow Spot Virus in organically grown onion crops in Canterbury and Hawke's Bay over three years

2018 ◽  
Vol 71 ◽  
pp. 39-44
Author(s):  
Melanie M. Davidson ◽  
Mette C. Nielson ◽  
John D. Fletcher
Keyword(s):  
New Zealand ◽  
Allium Cepa ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus ◽  
Potential Factors ◽  
Organically Grown ◽  
Organically Managed

The Tospovirus Iris yellow spot virus (IYSV), transmitted by thrips (predominantly Thrips tabaci), was first recorded in New Zealand in 2007. In March 2015, symptoms of the virus were relatively widespread in an organically managed onion crop in Canterbury. Onion plants were sampled for the presence of T. tabaci adults and larvae and for IYSV symptoms on an organically managed farm in Canterbury in 2014-2015, 2015-16 and 2016-17, and on a similar farm in Hawke's Bay in 2014-2015 and 2015-16. An immunoassay was used to confirm the presence of IYSV in some symptomatic plants. In Canterbury, IYSV symptoms were less apparent in 2015-16 and no symptoms were observed in 2016-17. No IYSV symptoms were observed in the Hawke's Bay onion crop, despite relatively high T. tabaci numbers. The virus symptoms declined from when they were first observed in March 2015 to undetectable levels in 2016-17 in Canterbury, which may be attributed to crop location, fewer thrips and the absence of a disease reservoir in volunteer Allium cepa plants or other hosts.

scholarly journals Distribución viral en plantas de cebolla (Allium cepa L.) asintomáticas

2018 ◽  
Vol 3 (7) ◽  
pp. 1425-1434 ◽  
Author(s):  
Rodolfo Velásquez-Valle ◽  
Manuel Reveles-Hernández ◽  
Mario Domingo Amador-Ramírez
Keyword(s):  
Tomato Spotted Wilt Virus ◽  
Onion Yellow Dwarf Virus ◽  
Leek Yellow Stripe Virus ◽  
Tomato Spotted Wilt ◽  
Allium Cepa L ◽  
Garlic Common Latent Virus ◽  
Yellow Dwarf Virus ◽  
Yellow Stripe ◽  
Wilt Virus ◽  
Das Elisa

Se estudió por medio de DAS- ELISA la distribución del Onion yellow dwarf virus (OYDV, virus del enanismo amarillo de la cebolla), Garlic common latent virus (GarCLV, virus latente común del ajo), Shallot latent v irus (SLV, virus latente del chalote), Leek yellow stripe virus (LYSV: virus de la franja amarilla del puerro), Tobacco etch virus (TEV, virus del jaspeado del tabaco) y Tomato spotted wilt virus (TSWV, virus de la marchitez manchada del jitomate) en hojas, psdeudotallo e inflorescencia de plantas de cebolla asintomáticas colectadas en diferentes épocas de 2010 en tres localidades del estado de Zacatecas, México. Se registró la presencia de esos virus en los órganos mencionados aunque resultó frecuente detectar infecciones mezcladas en hojas individuales donde las interacciones más comunes involucraban dos, tres y cuatro virus. No se encontró una tendencia que relacione la presencia viral con los órganos vegetales analizados o con la edad de las hojas; sin embargo, las infecciones con un solo virus parecen ser menos frecuentes en las hojas de edad intermedia.

scholarly journals Hospedantes alternos de Iris yellow spot virus y trips en cultivos de cebolla de Morelos y Michoacán, México

2017 ◽  
Vol 35 (2) ◽  
Author(s):  
Norma Ávila Alistac ◽  
Sergio Ramírez Rojas ◽  
Ángel Rebollar Alviter ◽  
Remigio Anastacio Guzmán Plazola
Keyword(s):  
Allium Cepa ◽  
Ricinus Communis ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Rt Pcr ◽  
Spot Virus ◽  
Das Elisa

<p>El objetivo de la investigación fue identificar hospedantes alternos de <em>Iris yellow spot virus</em> (IYSV) y establecer el rango de hospedantes del putativo vector(es) en regiones productoras de cebolla (<em>Allium cepa</em>) de Morelos y Michoacán, México. En 10 localidades de ambos estados se muestrearon cultivos de cebolla y arvenses, en presencia y ausencia del cultivo. Las plantas de cebolla se analizaron por RT-PCR y las arvenses por DAS-ELISA. Las arvenses se identificaron a nivel especie, los trips colectados de las mismas se establecieron colonias para su identificación por PCR, con iniciadores específicos que amplifican un segmento del gen de citocromo oxidasa I (COI). Se analizaron e identificaron 295 arvenses agrupadas en 56 especies (23 familias), todas resultaron negativas para IYSV. Se detectaron trips en 75 arvenses agrupadas en 17 especies. Se analizaron 33 poblaciones de trips (22 de Morelos y 11 de Michoacán). La secuenciación indicó identidad con <em>Thrips tabaci</em> con una homología superior a 97 %. Las arvenses <em>Ricinus communis</em> y <em>Acalypha ostryifolia</em> registraron el mayor número de <em>T. tabaci</em>. En cebolla se confirmó la presencia de IYSV con RT-PCR en las 10 parcelas muestreadas. Este es el primer reporte de la presencia de IYSV en el estado de Michoacán.</p>

scholarly journals DETECCIÓN DEL IRIS YELLOW SPOT VIRUS EN EL CULTIVO DE CEBOLLA EN ZACATECAS, MÉXICO

2018 ◽  
Vol 2 (6) ◽  
pp. 971-978
Author(s):  
Rodolfo Velásquez-Valle ◽  
Manuel Reveles-Hernández
Keyword(s):  
Frankliniella Occidentalis ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Spot Virus ◽  
La Mancha ◽  
Das Elisa

El virus de la mancha amarilla del iris (IYSV) es la enfermedad viral de mayor importancia para los cultivos de ajo y cebolla en Zacatecas, México. A finales de mayo de 2010 se encontraron lesiones amarillentas en forma de diamante en hojas y tallo floral de plantas de cebolla en parcelas comerciales, localizados en los municipios de Loreto, Villa de Cos, Sain Alto, Villanueva, Calera de V. R. y Enrique Estrada pertenecientes al estado de Zacatecas. La prueba DAS-ELISA mostró resultados positivos para este virus en las muestras procedentes de diferentes municipios. Thrips tabaci el único vector conocido del IYSV se encontró en las parcelas comerciales muestreadas, aunque la presencia de Frankliniella occidentalis también fue reconocida en una parcela comercial de cebolla, cuyas muestras resultaron positivas a IYSV. No se encontró diferencia entre la altura, número de hojas, peso y diámetro de bulbo de plantas sin lesiones y aquellas con diferente número de lesiones de IYSV.

scholarly journals Hospedantes alternos de Iris yellow spot virus y trips en cultivos de cebolla de Morelos y Michoacán, México

2017 ◽  
Vol 35 (2) ◽  
Author(s):  
Norma Ávila Alistac ◽  
Sergio Ramírez Rojas ◽  
Ángel Rebollar Alviter ◽  
Remigio Anastacio Guzmán Plazola
Keyword(s):  
Allium Cepa ◽  
Ricinus Communis ◽  
Thrips Tabaci ◽  
Iris Yellow Spot Virus ◽  
Yellow Spot ◽  
Rt Pcr ◽  
Spot Virus ◽  
Das Elisa

<p>El objetivo de la investigación fue identificar hospedantes alternos de <em>Iris yellow spot virus</em> (IYSV) y establecer el rango de hospedantes del putativo vector(es) en regiones productoras de cebolla (<em>Allium cepa</em>) de Morelos y Michoacán, México. En 10 localidades de ambos estados se muestrearon cultivos de cebolla y arvenses, en presencia y ausencia del cultivo. Las plantas de cebolla se analizaron por RT-PCR y las arvenses por DAS-ELISA. Las arvenses se identificaron a nivel especie, los trips colectados de las mismas se establecieron colonias para su identificación por PCR, con iniciadores específicos que amplifican un segmento del gen de citocromo oxidasa I (COI). Se analizaron e identificaron 295 arvenses agrupadas en 56 especies (23 familias), todas resultaron negativas para IYSV. Se detectaron trips en 75 arvenses agrupadas en 17 especies. Se analizaron 33 poblaciones de trips (22 de Morelos y 11 de Michoacán). La secuenciación indicó identidad con <em>Thrips tabaci</em> con una homología superior a 97 %. Las arvenses <em>Ricinus communis</em> y <em>Acalypha ostryifolia</em> registraron el mayor número de <em>T. tabaci</em>. En cebolla se confirmó la presencia de IYSV con RT-PCR en las 10 parcelas muestreadas. Este es el primer reporte de la presencia de IYSV en el estado de Michoacán.</p>

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