Characterization of Cellular Growth, CO2 Assimilation and Neutral Lipid Production for 4 Different Algal Species

AbstractSupplementing cultivation media with exogenous carbon sources enhances biomass and lipid production in microalgae. Utilization of renewable organic carbon from agricultural residues can potentially reduce the cost of algae cultivation, while enhancing sustainability. In the present investigation a medium was developed from sweet sorghum bagasse for cultivation of Chlorella under mixotrophic conditions. Using response surface methodology, the optimal values of critical process parameters were determined, namely inoculum cell density (O.D.750) of 0.786, SSB hydrolysate content of the medium 25% v/v, and zero medium salinity, to achieve maximum lipid productivity of 120 mg/L/d. Enhanced biomass (3.44 g/L) and lipid content (40% of dry cell weight) were observed when the alga was cultivated in SSB hydrolysate under mixotrophic conditions compared to heterotrophic and photoautotrophic conditions. A time course investigation revealed distinct physiological responses in terms of cellular growth and biochemical composition of C. vulgaris cultivated in the various trophic modes. The determined carbohydrate and lipid profiles indicate that sugar addition to the cultivation medium boosts neutral lipid synthesis compared to structural lipids, suggesting that carbon flux is channeled towards triacylglycerol synthesis in the cells. Furthermore, the fatty acid profile of lipids extracted from mixotrophically grown cultures contained more saturated and monosaturated fatty acids, which are suitable for biofuel manufacturing. Scale-up studies in a photobioreactor using SSB hydrolysate achieved a biomass concentration of 2.83 g/L consisting of 34% lipids and 26% carbohydrates. These results confirmed that SSB hydrolysate is a promising feedstock for mixotrophic cultivation of Chlorella and synthesis of algal bioproducts and biofuels.

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Fine Tuning of Redox Networks on Multiheme Cytochromes fromGeobacter sulfurreducensDrives Physiological Electron/Proton Energy Transduction

Bioinorganic Chemistry and Applications ◽

10.1155/2012/298739 ◽

2012 ◽

Vol 2012 ◽

pp. 1-9 ◽

Cited By ~ 17

Author(s):

Leonor Morgado ◽

Joana M. Dantas ◽

Marta Bruix ◽

Yuri Y. Londer ◽

Carlos A. Salgueiro

Keyword(s):

Microbial Fuel Cells ◽

Geobacter Sulfurreducens ◽

Cellular Growth ◽

Bohr Effect ◽

Fine Tuning ◽

Physiological Relevance ◽

Ph Range ◽

Coupling Mechanisms ◽

Redox Bohr Effect

The bacteriumGeobacter sulfurreducens (Gs)can grow in the presence of extracellular terminal acceptors, a property that is currently explored to harvest electricity from aquatic sediments and waste organic matter into microbial fuel cells. A family composed of five triheme cytochromes (PpcA-E) was identified inGs. These cytochromes play a crucial role by bridging the electron transfer from oxidation of cytoplasmic donors to the cell exterior and assisting the reduction of extracellular terminal acceptors. The detailed thermodynamic characterization of such proteins showed that PpcA and PpcD have an important redox-Bohr effect that might implicate these proteins in the e−/H+coupling mechanisms to sustain cellular growth. The physiological relevance of the redox-Bohr effect in these proteins was studied by determining the fractional contribution of each individual redox-microstate at different pH values. For both proteins, oxidation progresses from a particular protonated microstate to a particular deprotonated one, over specific pH ranges. The preferred e−/H+transfer pathway established by the selected microstates indicates that both proteins are functionally designed to couple e−/H+transfer at the physiological pH range for cellular growth.

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Neutral lipid storage disease with myopathy: Further phenotypic characterization of a rare PNPLA2 variant

Neuromuscular Disorders ◽

10.1016/j.nmd.2018.04.010 ◽

2018 ◽

Vol 28 (7) ◽

pp. 606-609 ◽

Cited By ~ 3

Author(s):

Caitlin S. Latimer ◽

Jennifer Schleit ◽

Adam Reynolds ◽

Desiree A. Marshall ◽

Benjamin Podemski ◽

...

Keyword(s):

Neutral Lipid ◽

Storage Disease ◽

Lipid Storage ◽

Phenotypic Characterization ◽

Lipid Storage Disease ◽

Neutral Lipid Storage Disease

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Selection and characterization of microalgae with potential for nutrient removal from municipal wastewater and simultaneous lipid production

Journal of Bioscience and Bioengineering ◽

10.1016/j.jbiosc.2019.12.004 ◽

2020 ◽

Vol 129 (5) ◽

pp. 565-572 ◽

Cited By ~ 10

Author(s):

Tsuyoshi Aketo ◽

Yumiko Hoshikawa ◽

Daisuke Nojima ◽

Yusuke Yabu ◽

Yoshiaki Maeda ◽

...

Keyword(s):

Nutrient Removal ◽

Municipal Wastewater ◽

Lipid Production

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Impact of Culture Conditions on Neutral Lipid Production by Oleaginous Yeast

Methods in Molecular Biology - Microbial Lipid Production ◽

10.1007/978-1-4939-9484-7_18 ◽

2019 ◽

pp. 311-325

Author(s):

Irene Fakankun ◽

Maryam Mirzaei ◽

David B. Levin

Keyword(s):

Neutral Lipid ◽

Oleaginous Yeast ◽

Lipid Production ◽

Culture Conditions

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Synthesis and characterization of fluorescent neutral lipid analogs containing N-(7-nitro-2,1,3-benzoxadiazol-4-yl)-aminohexanoic acid

Chemistry and Physics of Lipids ◽

10.1016/0009-3084(93)90002-k ◽

1993 ◽

Vol 66 (3) ◽

pp. 171-180 ◽

Cited By ~ 5

Author(s):

Jonathan C. McIntyre ◽

David Watson ◽

Richard G. Sleight

Keyword(s):

Neutral Lipid ◽

Synthesis And Characterization

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Detection, identification and mapping of cyanobacteria — Using remote sensing to measure the optical quality of turbid inland waters

Marine and Freshwater Research ◽

10.1071/mf9940801 ◽

1994 ◽

Vol 45 (5) ◽

pp. 801 ◽

Cited By ~ 74

Author(s):

DLB Jupp ◽

JTO Kirk ◽

GP Harris

Keyword(s):

Remote Sensing ◽

Algal Species ◽

Optical Quality ◽

High Spectral Resolution ◽

Turbid Waters ◽

Eastern Australia ◽

Processing Techniques ◽

Compact Airborne Spectrographic Imager

The advantages of airborne scanning for the detection, identification and mapping of algal species, cyanobacteria and associated water parameters (such as turbidity) can be realized if current research outcomes are developed into operational methods based on images with high spectral resolution. Evidence for this has become available through data obtained recently in Australia from the Compact Airborne Spectrographic Imager. This paper shows how pigments associated with cyanobacteria are detectable, even in the very turbid waters typical of eastern Australia. It demonstrates how, if the waterbodies and their constituents can be characterized by a programme of field and laboratory measurement, current processing techniques and models allow the concentrations of different constituents (algae and particles) in the photic zone to be estimated and mapped. The challenge for operational remote sensing of optical water quality in Australia (and throughout the world) is seen to have two components. One is to provide an effective characterization of the target inland and adjacent coastal waters and the other is to streamline the data analysis to provide maps of water properties in the time and cost frameworks required for operational use.

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Isolation and characterization of neutral-lipid-containing organelles and globuli-filled plastids from Brassica napus tapetum

Proceedings of the National Academy of Sciences ◽

10.1073/pnas.94.23.12711 ◽

1997 ◽

Vol 94 (23) ◽

pp. 12711-12716 ◽

Cited By ~ 67

Author(s):

S. S. H. Wu ◽

K. A. Platt ◽

C. Ratnayake ◽

T.-W. Wang ◽

J. T. L. Ting ◽

...

Keyword(s):

Brassica Napus ◽

Neutral Lipid ◽

Isolation And Characterization

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Characterization of a murine gene encoding a developmentally regulated cytoplasmic dual-specificity mitogen-activated protein kinase phosphatase

Biochemical Journal ◽

10.1042/bj3640145 ◽

2002 ◽

Vol 364 (1) ◽

pp. 145-155 ◽

Cited By ~ 38

Author(s):

Robin J. DICKINSON ◽

David J. WILLIAMS ◽

David N. SLACK ◽

Jill WILLIAMSON ◽

Ole-Morten SETERNES ◽

...

Keyword(s):

Nuclear Export ◽

Mammalian Cells ◽

Critical Role ◽

Nuclear Export Signal ◽

Cellular Growth ◽

Cell Fate Decisions ◽

Isolation And Characterization ◽

Dual Specificity ◽

Mitogen Activated Protein

Mitogen-activated protein kinases (MAPKs) play a vital role in cellular growth control, but far less is known about these signalling pathways in the context of embryonic development. Duration and magnitude of MAPK activation are crucial factors in cell fate decisions, and reflect a balance between the activities of upstream activators and specific MAPK phosphatases (MKPs). Here, we report the isolation and characterization of the murine Pyst3 gene, which encodes a cytosolic dual-specificity MKP. This enzyme selectively interacts with, and is catalytically activated by, the ‘classical’ extracellular signal-regulated kinases (ERKs) 1 and 2 and, to a lesser extent, the stress-activated MAPK p38α. These properties define the ability of this enzyme to dephosphorylate and inactivate ERK1/2 and p38α, but not JNK (c-Jun N-terminal kinase) in vivo. When expressed in mammalian cells, the Pyst3 protein is predominantly cytoplasmic. Furthermore, leptomycin B causes a complete redistribution of the protein to the nucleus, implicating a CRM (chromosomal region maintenance)1/exportin 1-dependent nuclear export signal in determining the subcellular localization of this enzyme. Finally, whole-mount in situ hybridization studies in mouse embryos reveal that the Pyst3 gene is expressed specifically in the placenta, developing liver and in migratory muscle cells. Our results suggest that this enzyme may have a critical role in regulating the activity of MAPK signalling during early development and organogenesis.

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