scholarly journals Design and construction of a localized surface plasmon resonance-based gold nanobiosensor for rapid detection of brucellosis

2021 ◽  
Vol 48 (3) ◽  
Author(s):  
Sina Vakili ◽  
◽  
Gholamreza Asadikaram ◽  
Masoud Torkzadeh-Mahani ◽  
Alimohammad Behroozikhah ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Brucella Melitensis ◽  
Localized Surface Plasmon ◽  
Human Sera ◽  
Phenol Water ◽  
Covalent Interactions

In this study, a localized surface plasmon resonance (LSPR) nanobiosensor was designed to quantify anti-Brucella antibodies in the human sera. Smooth Lipopolysaccharide (LPS) was extracted from Brucella melitensis via a modified hot phenol water method and fixed on the surface of the gold nanoparticles by covalent interactions with the functionalized nanoparticles. To obtain the best performance from the designed probe, the ratio of LPS to gold nanoparticle was optimized. Dynamic light scattering was used for the characterization of the probe. The reduction of the LSPR peak at 600nm was used to quantify the amount of captured anti-Brucella antibody. Finally, satisfactory results were obtained when the nanobiosensor was used to analyze the control and patient sera for the presence of anti-Brucella antibodies.

Development and Characterization of a Diamond-Based Localized Surface Plasmon Resonance Interface

2010 ◽  
Vol 114 (8) ◽  
pp. 3346-3353 ◽  
Author(s):  
Sabine Szunerits ◽  
Slimane Ghodbane ◽  
Joanna Niedziółka-Jönsson ◽  
Elisabeth Galopin ◽  
Frederik Klauser ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Localized Surface Plasmon

scholarly journals Gold Nanobiosensor Based on the Localized Surface Plasmon Resonance is Able to Diagnose Human Brucellosis, Introducing a Rapid and Affordable Method

2021 ◽  
Vol 16 (1) ◽  
Author(s):  
Sina Vakili ◽  
Mohammad Samare-Najaf ◽  
Amirreza Dehghanian ◽  
Amir Tajbakhsh ◽  
Hassan Askari ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Predictive Value ◽  
Localized Surface Plasmon Resonance ◽  
Brucella Melitensis ◽  
Localized Surface Plasmon ◽  
Human Brucellosis ◽  
True Negative ◽  
Significant Difference

AbstractBrucellosis is considered as the most common bacterial zoonosis in the world. Although the laboratory findings are the most reliable diagnosis today, the current laboratory methods have many limitations. This research aimed to design and evaluate the performance of a novel technique based on the localized surface plasmon resonance (LSPR) to eliminate or reduce existing shortcomings. For this purpose, smooth lipopolysaccharides were extracted from Brucella melitensis and Brucella abortus and fixed on the surface of the gold nanoparticles through covalent interactions. After some optimizing processes, dynamic light scattering was used to characterize the probe. The detection of captured anti-Brucella antibody was performed by measuring the redshift on LSPR peak followed by the determination of cutoff value, which indicated a significant difference between controls and true positive patients (P value < 0.01). Furthermore, 40 sera from true negative samples and positive patients were used to evaluate the performance of this method by comparing its outcomes with the gold standard (culture), standard tube agglutination test, and anti-brucellosis IgM and IgG levels (ELISA). The sensitivity, specificity, positive predictive value, and negative predictive value showed an appropriate performance of the LSPR-based method (85%, 100%, 100%, and 86%, respectively). The current research results provide a promising fast, convenient, and inexpensive method for detecting the anti-Brucella antibodies in human sera, which can be widely used in medical laboratories to diagnose brucellosis quickly and effectively.

Fabrication of Gold Nanocrescents by Angle Deposition with Nanosphere Lithography for Localized Surface Plasmon Resonance Applications

2008 ◽  
Vol 8 (7) ◽  
pp. 3369-3378 ◽  
Author(s):  
Xiaodong Zhou ◽  
Selven Virasawmy ◽  
Wolfgang Knoll ◽  
Kai Yu Liu ◽  
Man Siu Tse ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Localized Surface Plasmon Resonance ◽  
Gold Film ◽  
Nanosphere Lithography ◽  
Localized Surface Plasmon ◽  
2D And 3D ◽  
Deposition Angle

The gold nanostructures fabricated on a substrate yield localized surface plasmon resonance. We describe the fabrication and characterization of nanocrescents on a silicon substrate, which are fabricated by depositing a gold film at an oblique angle through nanosphere lithography. Following the etching of the gold perpendicular to the substrate and the removal of the nanospheres by dissolution, nanocrescents with fine nanostructures are generated. By varying the deposition angle of the gold film from 0° to 72°, nanorings, 2D and 3D nanocrescents can be obtained. During the nanocrescent fabrication, we also compared the deposition angle difference between the e-beam and thermal evaporators for oblique depositions of the gold. The 3D nanocrescents fabricated in our experiments are expected to have improved sensitivity in localized surface plasmon resonance measurements when compared to the previously reported 2D nanocrescents, which enable broader biosensor applications. Simulations of the profiles of these 3D nanocrescents using solid geometry show good consistency with the fabricated ones.

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