scholarly journals Transabdominal Ultrasound-guided Follicular Aspiration in Case of Müllerian Anomaly with Ovarian Malposition

2017 ◽  
Vol 9 (1) ◽  
pp. 53-55
Author(s):  
Preeti A Goyal
Keyword(s):  
Oocyte Retrieval ◽  
Controlled Ovarian Hyperstimulation ◽  
Fertilization Rate ◽  
Transabdominal Ultrasound ◽  
Ultrasound Guided ◽  
Vitro Fertilization ◽  
Follicular Aspiration ◽  
Mullerian Anomaly ◽  
Embryo Number

ABSTRACT Objectives: To describe the approach of transabdominal ultrasound-guided oocyte retrieval in a patient of Müllerian anomaly and ovarian malposition. Design: Case report. Setting A tertiary referral reproductive medicine unit. Patient A 23-year-old patient with Müllerian anomaly (right unicornuate with left rudimentary horn uterus) and ovarian malposition. Intervention Controlled ovarian hyperstimulation, transabdominal ultrasound-guided oocyte retrieval. Main outcome measure Total and mature oocytes retrieved, fertilization rate, embryo number, and quality. Results Five oocytes were retrieved transabdominally, using percutaneous needle puncture under ultrasound guidance. Conclusion In in vitro fertilization protocols, patients with Müllerian anomaly with vaginally inaccessible ovaries, transabdominal ultrasound-guided follicular aspiration is a safe and efficacious alternative technique for oocyte retrieval. How to cite this article Mishra VV, Choudhary S, Goyal PA, Aggarwal R. Transabdominal Ultrasound-guided Follicular Aspiration in Case of Müllerian Anomaly with Ovarian Malposition. J South Asian Feder Obst Gynae 2017;9(1):53-55.

scholarly journals Transabdominal Ultrasound-guided Follicular Aspiration in Case of Müllerian Anomaly with Ovarian Malposition

2015 ◽  
Vol 7 (3) ◽  
pp. 213-215
Author(s):  
Vineet V Mishra ◽  
Preeti A Goyal
Keyword(s):  
Oocyte Retrieval ◽  
Controlled Ovarian Hyperstimulation ◽  
Fertilization Rate ◽  
Transabdominal Ultrasound ◽  
Ultrasound Guided ◽  
Vitro Fertilization ◽  
Follicular Aspiration ◽  
Mullerian Anomaly ◽  
Embryo Number

ABSTRACT Objective To describe the approach of transabdominal ultrasound-guided oocyte retrieval in a patient of müllerian anomaly and ovarian malposition. Setting A tertiary referral reproductive medicine unit. Patient A 23-year-old patient with müllerian anomaly (right unicornuate with left rudimentary horn uterus) and ovarian malposition. Intervention Controlled ovarian hyperstimulation, transabdominal ultrasound-guided oocyte retrieval. Main outcome measure Total and mature oocytes retrieved, fertilization rate, embryo number and quality. Result Five oocytes were retrieved transabdominally using percutaneous needle puncture under ultrasound guidance. Conclusion In in vitro fertilization (IVF) protocols, patients with müllerian anomaly with inaccessible ovaries vaginally, transabdominal ultrasound-guided follicular aspiration is a safe and efficacious alternative technique for oocyte retrieval. How to cite this article Mishra VV, Choudhary S, Goyal PA, Aggarwal R. Transabdominal Ultrasound-guided Follicular Aspiration in Case of Müllerian Anomaly with Ovarian Malposition. J South Asian Feder Obst Gynae 2015;7(3):213-215.

Clinical results from intracytoplasmic sperm injection at monash IVF

1995 ◽  
Vol 7 (2) ◽  
pp. 247 ◽  
Author(s):  
RI McLachlan ◽  
G Fuscaldo ◽  
H Rho ◽  
C Poulos ◽  
J Dalrymple ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Oocyte Retrieval ◽  
Clinical Results ◽  
Fertilization Rate ◽  
Vitro Fertilization ◽  
Failed Fertilization ◽  
Patient Groups ◽  
Sperm Antibodies ◽  
The Impact

The impact of a modification of the intracytoplasmic sperm injection (ICSI) technique on fertilization and pregnancy rates was examined in a retrospective analysis of 171 consecutive ICSI treatment cycles (156 patients). Patients were selected for ICSI on the basis of severe oligoasthenozoospermia (65 patients) or following conventional in vitro fertilization (IVF) with failed or poor fertilization (70 patients). Seven patients in which epididymal or testicular sperm was used, 10 patients with sperm antibodies and 4 patients with retrograde ejaculation or who required electro-ejaculation were also treated with ICSI. In the first 105 cycles (102 patients), single sperm, rendered immotile, were injected into the ooplasm of 979 metaphase II (M II) oocytes using an established technique (Method 1). In the following 66 cycles (513 M II oocytes injected), the ICSI procedure was modified by increased aspiration of the oolemma to ensure the intracytoplasmic deposition of sperm (Method 2). The patient groups did not differ between the two injection procedures. The normal (two pronuclear) fertilization rate increased significantly (P < 0.001) from 34.3% with Method 1 to 73.1% with Method 2, with no difference in the oocyte degeneration rate (4.3% v. 4.5% respectively). The incidence of failed fertilization was significantly (P < 0.01) reduced from 17.1% (18 cycles) to 1.6% (1 cycle) with the change in technique. As a consequence of the increased fertilization rates with Method 2, more embryos were available for assessment and transfer, and a pregnancy rate per oocyte retrieval of 21.2% was obtained for Method 2. Fertilization, embryo transfer and pregnancies were obtained in all patient groups treated with ICSI.(ABSTRACT TRUNCATED AT 250 WORDS)

scholarly journals Does Unilateral Oocyte Retrieval due to Transvaginally Inaccessible Ovaries, Contrary to Common Beliefs, Affect IVF/ICSI Treatment Outcomes That Much?

2016 ◽  
Vol 2016 ◽  
pp. 1-6
Author(s):  
Safak Olgan ◽  
Sezcan Mumusoglu ◽  
Gurkan Bozdag
Keyword(s):  
Treatment Outcomes ◽  
Oocyte Retrieval ◽  
Antral Follicle ◽  
Antral Follicle Count ◽  
Clinical Pregnancy ◽  
Roc Curve Analysis ◽  
Vitro Fertilization ◽  
Follicular Aspiration ◽  
Single Ovary

Objective. To investigate in vitro fertilization (IVF) treatment outcomes of unilateral oocyte retrieval in patients with transvaginally inaccessible ovaries.Study Design. Ninety-two women who underwent unilateral oocyte retrieval were retrospectively matched for age, antral follicle count, and body mass index with 184 women who underwent bilateral oocyte retrieval. Each patient in bilateral oocyte retrieval group had the same number of cumulus oophorus complexes (COCs) from single ovary and had comparable number of follicles (±2) on contralateral site where follicular aspiration was performed.Results. The number of COCs, metaphase-2 oocytes, 2-pronuclei, and top-quality embryos was significantly lower in unilateral oocyte retrieval group. However, proportion of patients with an embryo transfer of at least one top-quality embryo was found to be comparable between unilateral and bilateral oocyte retrieval. Subsequently, clinical pregnancy and live birth rates were found to be similar between the groups. The ROC curve analysis revealed (AUC = 0.74, 95% CI 0.63–0.86,p=0.001) that retrieved COCs ≥ 5 from single ovary had sensitivity of 76.0% and specificity of 64.2% for occurrence of a clinical pregnancy.Conclusion. The patients with unilateral oocyte retrieval have reasonable chance of success with IVF. The retrieval of ≥5 COCs from accessible ovary might result in better treatment outcomes among these patients.

263 IN VITRO FERTILIZATION AND EMBRYO DEVELOPMENT OF CUMULUS - OOCYTE COMPLEXES COLLECTED BY ULTRASOUND-GUIDED FOLLICULAR ASPIRATION IN LLAMAS TREATED WITH GONADOTROPIN

2010 ◽  
Vol 22 (1) ◽  
pp. 288 ◽  
Author(s):  
M. A. Berland ◽  
A. von Baer ◽  
V. Parraguez ◽  
P. Morales ◽  
G. P. Adams ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Animal Health ◽  
Ovarian Response ◽  
Ultrasound Guided ◽  
Vitro Fertilization ◽  
Follicular Aspiration ◽  
Oocyte Collection ◽  
Health Canada

We have previously documented that both FSH and eCG are equally effective in inducing ovarian superstimulation in llamas, resulting in the recovery of a high number of expanded COC suitable for in vitro fertilization (Ratto et al. 2005 Theriogenology 63, 2445-2457). The objective of the study was to evaluate the ovarian response, morphology, and competence of COC collected by ultrasound-guided follicular aspiration in llamas treated with FSH or eCG. Llamas were assigned randomly into 2 groups (n = 16 per group) and treated for 48 h after follicle ablation with (1)25 mg of FSH (Folltropin, Bioniche Animal Health Canada Inc., Belleville, Canada) i.m. twice daily for 4 d; or (2) 1000IU of eCG (Novormon, Bioniche Animal Health Canada) as a single i.m. dose. The starting of gonadotropin treatment was considered Day 0. Both groups were given an i.m. dose of 5 mg of Armour Standard LH (Lutropin, Bioniche Animal Health Canada) on Day 6, and COC were collected by transvaginal ultrasound follicle aspiration of all follicles ≥7 mm on Day 7. The ovarian response was assessed by transrectal ultrasonography using a 7.5-MHz linear-array transducer (Aloka SSD-500, Clinics, Santiago, Chile) immediately before oocyte collection at 24 to 26 h after LH treatment in both groups. The COC were classified as expanded, compact, denuded, or degenerated. Expanded COC collected from FSH- (n = 147) and eCG-treated llamas (n = 141) were fertilized in vitro using epididymal sperm as previously described (Ratto et al. 2006 Anim. Reprod. Sci. 97, 246-257). Gametes were co-incubated at 38.5°C in air with 5% CO2 and high humidity for 18 h. After in vitro fertilization, presumptive zygotes were co-culture in SOF medium supplemented with 0.6% of BSA with llama granulosa cells at 39°C, 5% CO2, 5% O2, and 90% N2 for 7 days. Embryo development was evaluated on Days 2, 5, and 7 of in vitro culture (Day 0 = IVF). Data were analyzed by Student’s t-test or Fisher’s exact test and presented as mean ± SEM. The FSH and eCG treatment groups did not differ with respect to the number of follicles ≥7 mm at the time of COC collection (16.0 ± 2.7 v. 14.0 ± 1.9; P = 0.5), the number of COC collected (11.5 ± 1.9 v. 9.7 ± 1.2; P = 0.4), or the collection rate per follicle aspirated (77.0 v. 71.5%; P = 0.2). No difference was detected between FSH and eCG-treated llamas in the number of expanded COCs (9.8 ± 1.4 v. 9.4 ± 1.2; P = 0.8). The percentage of presumptive zygotes to develop into 2 to 8 cells on Day 2 (65.3 v. 63.1), morulas on Day 5 (46.2 v. 42.5), and blastocyst stage on Day 7 (23.1 v. 20.5) did not differ (P > 0.05) between FSH and eCG-treated llamas, respectively. In conclusion, FSH and eCG treatments were equally effective for ovarian superstimulation and oocyte collection. The recovery of a high number of expanded COC can be used directly for in vitro fertilization and their competence is not affected by gonadotropin treatment. The study was supported by Convenio Desempeño en Investigacion (2007-DGI-CDA-04), Universidad Catolica de Temuco.

scholarly journals Prosaposin in seminal plasma on the day of oocyte retrieval is associated with normal fertilization and embryo development in in vitro fertilization cycles

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e8177
Author(s):  
Chun Xu ◽  
Jiali Cai ◽  
Lanlan Liu ◽  
Jianzhi Ren
Keyword(s):  
In Vitro Fertilization ◽  
Embryo Development ◽  
Sperm Concentration ◽  
Oocyte Retrieval ◽  
Estimating Equation ◽  
Fertilization Rate ◽  
Semen Parameters ◽  
Vitro Fertilization ◽  
Normal Fertilization

The prospective study including 166 participants aims to evaluate the association between seminal prosaposin and the outcomes of in vitro fertilization (IVF) cycles in humans. The generalized linear model (GLM) was used to analyze the associations between seminal prosaposin concentrations and normal fertilization rates and good embryos proportion. The generalized estimating equation (GEE) was used to evaluate the association between embryo parameters and the prosaposin concentrations. Each model was adjusted for age of the couples, female basal FSH, AFC and BMI, starting dose and oocyte yield of IVF cycles and smoker. GLM models suggested that prosaposin was significantly associated with fertilization rate (P = 0.005) and good embryo proportion (P = 0.038) while none of the semen parameters (sperm concentration, motility, progressive motility, normal morphology rate, postwash sperm concentration and motility) was significantly associated with the parameters in the cohort. Using GEE, it was also shown that prosaposin was positively associated with the occurrence of early cleavage and negatively associated with uneven cleavage pattern on day 3. In both the overall population and the normozoospermia patients, the prosaposin was significantly associated with pregnancy with adjustment with covariates. In conclusion, our data suggested that seminal prosaposin concentration could provide more information regarding normal fertilization and embryo development in IVF than traditional semen parameters.

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