scholarly journals Efficiency of microspore embryogenesis in Brassica rapa using different genotypes and culture conditions

2014 ◽  
Vol 41 (3) ◽  
pp. 116-122 ◽  
Author(s):  
Mi-Suk Seo ◽  
Seong-Han Sohn ◽  
Beom-Seok Park ◽  
Ho-Cheol Ko ◽  
Mina Jin
Keyword(s):  
Brassica Rapa ◽  
Microspore Embryogenesis ◽  
Culture Conditions

Effect of thidiazuron on microspore embryogenesis and plantlet regeneration in Chinese flowering cabbage ( Brassica rapa. var. parachinenis )

2019 ◽  
Vol 138 (6) ◽  
pp. 916-924
Author(s):  
JunXiang Jia ◽  
Yun Zhang ◽  
LianWei Cui ◽  
Hui Feng
Keyword(s):  
Brassica Rapa ◽  
Microspore Embryogenesis ◽  
Plantlet Regeneration

scholarly journals Effects of Genotype and Culture Conditions on Microspore Embryogenesis and Plant Regeneration in Brassica Rapa ssp. Rapa L.

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 278 ◽  
Author(s):  
Daria Shumilina ◽  
Dmitry Kornyukhin ◽  
Elena Domblides ◽  
Alexey Soldatenko ◽  
Anna Artemyeva
Keyword(s):  
Microspore Culture ◽  
Doubled Haploids ◽  
Genetic Disorder ◽  
Cold Treatment ◽  
Microspore Embryogenesis ◽  
Culture Conditions ◽  
Secondary Embryogenesis ◽  
Isolated Microspore Culture ◽  
Pure Lines

Turnip is a biennial crop and, consequently, the creation of pure lines for breeding is a time-consuming process. The production of pure turnip lines using doubled haploids produced in isolated microspore culture has not been sufficiently developed. The aim of the present work was to determine some key factors inducing embryogenesis in the isolated microspore culture of turnip, as well as investigating the manners of embryo development. It was shown that the acidity of the medium is an important factor in embryo production; different optimal pH levels ranging from 6.2 to 6.6 corresponded to individual genotypes. Such factors as the cold treatment of buds and the addition of activated charcoal to the nutrient medium increased the responsiveness of all genotypes studied. The turnip variety ‘Ronde witte roodkop herfst’ demonstrated a genetic disorder in the development of microspores; namely, non-separation of some microspores from tetrads. In the in vitro culture, each of the daughter microspores developed on its own. This indicates the dependence of the possibility of embryogenesis in the turnip microspore culture on the genotype. Results suggest that the initiation of secondary embryogenesis in primary embryos leads to an increase in the proportion of doubled haploid plants.

scholarly journals QTL Analysis of the Content of Some Bioactive Compounds in Brassica rapa L. Grown under Light Culture Conditions

Horticulturae ◽  
2021 ◽  
Vol 7 (12) ◽  
pp. 583
Author(s):  
Ksenia V. Egorova ◽  
Nadezhda G. Sinyavina ◽  
Anna M. Artemyeva ◽  
Natalia V. Kocherina ◽  
Yuriy V. Chesnokov
Keyword(s):  
Molecular Markers ◽  
Brassica Rapa ◽  
Doubled Haploid ◽  
Biochemical Composition ◽  
Dry Matter ◽  
Culture Conditions ◽  
Biologically Active Compounds ◽  
Biologically Active ◽  
Active Compounds ◽  
Doubled Haploid Lines

The article presents the results of biochemical and QTL (Quantitative Trait Loci) analysis of dry matter content, nutrient and biologically active compounds: sugars, ascorbic acid, chlorophylls a and b, anthocyanins and carotenoids in populations of doubled haploid lines of leaf, root crops, and oilseeds of the Brassica rapa L. species grown in optimal light culture conditions, but with different photoperiod durations. The purpose of this study was to evaluate the effect of the photoperiod on the transition to bolting and the accumulation of biologically active substances, as well as how the localization and identification of chromosomal loci determined the content of certain phytochemicals. The influence of the length of daylight hours on the content of components of the biochemical composition was assessed. It was shown that growing under conditions of a 16 h photoperiod increased the content of dry matter, sugars, vitamin C, and anthocyanins. On the contrary, the content of photosynthetic pigments was higher under the conditions of a 12 h photoperiod. Valuable lines that can be sources of biologically active compounds were revealed. Based on the results of the obtained data, 102 QTLs were mapped, which determine the manifestation of the studied biochemical quality traits in the B. rapa doubled haploid lines under conditions of short and long daylight hours. Molecular markers genetically linked to the selected QTLs were determined. It was revealed that the identified loci controlling all the studied biochemical traits were mainly in the fifth, sixth, seventh, and ninth linkage groups, which correlated with the data obtained in the field and greenhouse. Most of the identified loci controlled several studied traits simultaneously. The identified QTLs and identified molecular markers are of interest for further study of the genetic control of the economically valuable traits determined by them and for the implementation of marker-assisted selection in B. rapa. The data obtained can be used in genetic and breeding work, including for the obtaining of new genotypes, lines and cultivars with a valuable biochemical composition, adapted for cultivation under specific photoperiodic conditions.

The effect of culture shaking on microspore embryogenesis and embryonic development in Pakchoi (Brassica rapa L. ssp. chinensis)

2013 ◽  
Vol 152 ◽  
pp. 70-73 ◽  
Author(s):  
Shuo Yang ◽  
Xueli Liu ◽  
Ying Fu ◽  
Xi Zhang ◽  
Yuefei Li ◽  
...  
Keyword(s):  
Embryonic Development ◽  
Brassica Rapa ◽  
Microspore Embryogenesis

scholarly journals Application of Cobalt Chloride and Silver Nitrate for Efficient Microspore Culture of Brassica rapa ssp.

2013 ◽  
Vol 23 (1) ◽  
Author(s):  
Khandakar Md. Rayhanul Kabir ◽  
Soon-Wook Kwon ◽  
Yong-Jin Park
Keyword(s):  
Silver Nitrate ◽  
Brassica Rapa ◽  
Plant Tissue ◽  
Culture Medium ◽  
Cobalt Chloride ◽  
Microspore Culture ◽  
Microspore Embryogenesis ◽  
Progressive Increase ◽  
Embryo Production ◽  
Embryogenic Response

The effects of ethylene antagonistic cobalt chloride and silver nitrate, on microspore embryogenesis were investigated using three different concentrations in the medium for three Korean cultivars (two non-heading and one heading) of Brassica rapa ssp. Inclusion of cobalt chloride (5 μM ) in the culture medium significantly improved embryo production in the non-heading cultivar (33 embryos/bud) with embryo yields being increased up to 32%. The addition of silver nitrate (0.1 mg/l) to the culture medium also showed a progressive increase in embryo yields in the non-heading cultivar (34 embryos/bud) with embryo yields being increased up to 36%. For the heading cultivars, the highest embryogenic response was 2.8 embryos/bud (Jo saeng Miho), following the addition of silver nitrate (0.1 mg/l) to the culture medium, whereas 2.4 embryos/bud were observed with the addition of 5 μM cobalt chloride to the culture medium. Plant Tissue Cult. & Biotech. 23(1): 1-10, 2013 (June)

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