scholarly journals Efficient shoot regeneration using cotyledon explants and Agrobacterium tumefaciens - mediated transformation of bottle gourd (Lagenaria sicraria Standl.)

2014 ◽  
Vol 41 (3) ◽  
pp. 140-145
Author(s):  
Soo-Yun Kim ◽  
Yul-Kyun Ahn ◽  
Yun-Chan Huh ◽  
Hye-Eun Lee ◽  
Do-Sun Kim
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Shoot Regeneration ◽  
Bottle Gourd ◽  
Cotyledon Explants

scholarly journals Agrobacterium-mediated Transformation of Cotyledon Explants of Bottle Gourd (Lagenaria siceraria S.)

HortScience ◽  
2004 ◽  
Vol 39 (4) ◽  
pp. 821E-822
Author(s):  
Jeung-Sul Han* ◽  
Chang Kil Kim
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Binary Vector ◽  
Bottle Gourd ◽  
Infection Frequency ◽  
Ms Medium ◽  
Lagenaria Siceraria ◽  
Cultivation Medium ◽  
Glufosinate Ammonium ◽  
Cotyledon Explants ◽  
Selection Medium

A procedure for producing transgenic bottle gourd plants by inoculating cotyledon explants with Agrobacterium tumefaciens strain AGL1 carrying a binary vector pCAMBIA3301, which contains glufosinate ammonium-resistant (bar) and the reporter (gus) genes, is describe. Infection was the most effective (highest infection frequency and index) when explants were co-cultivated with Agrobacterium for 6-8 days on co-cultivation medium supplemented with 0.001-0.1 mg/L L-a-(2-aminoethoxyvinyl) glycine (AVG). Transgenic plants were obtained with frequencies of about 0.2% when the explants were cultured on selection medium (MS medium supplemented with 3.0 mg/L BAP, 0.5 mg/L AgNO3, 500 mg/L cefotaxime, 2.0 mg/L DL-phosphinothricin, 0.3% sucrose and 0.8% Plant Agar. A histochemical gus assay, PCR and Southern blot analyses confirmed that transformation had occurred. Genetic analysis of T1 progenies showed that the transgenes were inherited in a Mendelian fashion. To our knowlege, this study represents the first report for Agrobacterium-mediated transformation in bottle gourd, rootstock for watermelon and other cucurbit crops in many countries.

scholarly journals The influence of silver thiosulfate and thidiazuron on shoot regeneration from cotyledon explants of Brassica napus

2012 ◽  
Vol 39 (3) ◽  
pp. 133-139 ◽  
Author(s):  
Kyung-Hee Roh ◽  
Bo-Kyung Kwak ◽  
Jong-Bum Kim ◽  
Kyeong-Ryeol Lee ◽  
Hyun-Uk Kim ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Shoot Regeneration ◽  
Silver Thiosulfate ◽  
Cotyledon Explants

scholarly journals The effect of carbon source on plant regeneration in tomato

2011 ◽  
Vol 32 (No. 1) ◽  
pp. 6-8 ◽  
Author(s):  
J. Gubiš ◽  
Z. Lajchová ◽  
L. Klčová
Keyword(s):  
Plant Regeneration ◽  
Carbon Source ◽  
Shoot Regeneration ◽  
Regeneration Capacity ◽  
Hypocotyl Explants ◽  
Cotyledon Explants ◽  
Tomato Cultivars ◽  
Number Of Shoots

 The effect of different tomato cultivars and different sugar types (sucrose, glucose and maltose) and concentrations (1.0, 2.0 and 3.0%) on shoot regeneration from aseptically grown hypocotyl and cotyledon explants were studied. Among sugar types, sucrose at a concentration of 3.0% induced the highest number of shoots from both types of explants. In hypocotyl explants, cv. Premium showed the best regeneration capacity (0.23 shoots per explant), and in cotyledon explants, cv. Hana produced the maximal number of shoots (0.43 or 0.37 for media with 2.0% or 3.0% sucrose, respectively).  

High frequency shoot regeneration from cotyledon explants of cucumber via organogenesis

2007 ◽  
Vol 112 (1) ◽  
pp. 2-8 ◽  
Author(s):  
N. Selvaraj ◽  
A. Vasudevan ◽  
M. Manickavasagam ◽  
S. Kasthurirengan ◽  
A. Ganapathi
Keyword(s):  
Shoot Regeneration ◽  
High Frequency ◽  
Cotyledon Explants

Shoot regeneration in stem expiants and its amenability to Agrobacterium tumefaciens mediated gene transfer in Brassica carinata

1992 ◽  
Vol 11 (7) ◽  
Author(s):  
S.B. Narasimhulu ◽  
P.B. Kirti ◽  
T. Mohapatra ◽  
Shyam Prakash ◽  
V.L. Chopra
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Gene Transfer ◽  
Shoot Regeneration ◽  
Brassica Carinata

scholarly journals Optimization of Agrobacterium tumefaciens-mediated transformation and shoot regeneration after co-cultivation of cabbage (Brassica oleracea subsp. capitata) cv. KY Cross with AtHSP101 gene

2010 ◽  
Vol 124 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Arash Rafat ◽  
Maheran Abd Aziz ◽  
Azmi Abd Rashid ◽  
Siti Nor Akmar Abdullah ◽  
Hossein Kamaladini ◽  
...  
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Brassica Oleracea ◽  
Shoot Regeneration

scholarly journals Interaction between Explant Size and Cultivar Affects Shoot Organogenic Competence of Watermelon Cotyledons

HortScience ◽  
2000 ◽  
Vol 35 (4) ◽  
pp. 749-750 ◽  
Author(s):  
Michael E. Compton
Keyword(s):  
Shoot Regeneration ◽  
Significant Interaction ◽  
Adventitious Shoots ◽  
Adventitious Shoot ◽  
Shoot Formation ◽  
Proximal Region ◽  
Specific Region ◽  
Adventitious Shoot Regeneration ◽  
Cotyledon Explants

Organic competence of different explant sizes and locations on watermelon seedlings was determined by calculating the percentage of cotyledon explants that produced adventitious shoots. About 52% (214/412) of explants prepared from the proximal region of cotyledons formed shoots, whereas only ≈6% (24/411) of distal explants did so. Shoot formation was limited to the proximal end of basal explants but was not restricted to any specific region on distal ones. The percentage of explants that produced harvestable shoots was greater from basal halves than basal quarters in `Sweet Gem', `Crimson Sweet', and `Minilee', but explant size did not affect adventitious shoot regeneration of `Yellow Doll', resulting in significant interaction between cultivar and explant size. This study indicates that cultivars that respond poorly to in vitro procedures may have fewer cells competent for shoot regeneration, requiring special care during explant preparation.

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