scholarly journals The acute effect of postural change on subcutaneous tissue blood flow in the hand after sympathetic denervation.

1983 ◽  
Vol 31 (3) ◽  
pp. 705-707
Author(s):  
M. Naito ◽  
K. Ogata ◽  
H. Chuman ◽  
H. Takeshita ◽  
M. Moriyama
Keyword(s):  
Blood Flow ◽  
Subcutaneous Tissue ◽  
Acute Effect ◽  
Sympathetic Denervation ◽  
Tissue Blood Flow ◽  
Postural Change

Effect of training on epinephrine-stimulated lipolysis determined by microdialysis in human adipose tissue

1995 ◽  
Vol 269 (6) ◽  
pp. E1059-E1066 ◽  
Author(s):  
B. Stallknecht ◽  
L. Simonsen ◽  
J. Bulow ◽  
J. Vinten ◽  
H. Galbo
Keyword(s):  
Blood Flow ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Tissue ◽  
Physical Work ◽  
Tissue Blood Flow ◽  
Epinephrine Infusion ◽  
Arterial Blood ◽  
Adipose Tissue Blood Flow ◽  
Subcutaneous Adipose

Trained humans (Tr) have a higher fat oxidation during submaximal physical work than sedentary humans (Sed). To investigate whether this reflects a higher adipose tissue lipolytic sensitivity to catecholamines, we infused epinephrine (0.3 nmol.kg-1.min-1) for 65 min in six athletes and six sedentary young men. Glycerol was measured in arterial blood, and intercellular glycerol concentrations in abdominal subcutaneous adipose tissue were measured by microdialysis. Adipose tissue blood flow was measured by 133Xe-washout technique. From these measurements adipose tissue lipolysis was calculated. During epinephrine infusion intercellular glycerol concentrations were lower, but adipose tissue blood flow was higher in trained compared with sedentary subjects (P < 0.05). Glycerol output from subcutaneous tissue (Tr: 604 +/- 322 nmol.100 g-1.min-1; Sed: 689 +/- 203; mean +/- SD) as well as arterial glycerol concentrations (Tr: 129 +/- 36 microM; Sed: 119 +/- 56) did not differ between groups. It is concluded that in intact subcutaneous adipose tissue epinephrine-stimulated blood flow is enhanced, whereas lipolytic sensitivity to epinephrine is the same in trained compared with untrained subjects.

Clinical biochemical tissue monitoring during ischaemia and reperfusion in major vascular surgery

2003 ◽  
Vol 40 (3) ◽  
pp. 289-291 ◽  
Author(s):  
S Klaus ◽  
KH Staubach ◽  
W Eichler ◽  
J Gliemroth ◽  
M Heringlake ◽  
...  
Keyword(s):  
Blood Flow ◽  
Vascular Surgery ◽  
Lower Limb ◽  
Subcutaneous Tissue ◽  
Aortic Surgery ◽  
Tissue Blood Flow ◽  
Limb Ischaemia ◽  
Additional Tool ◽  
Acute Changes ◽  
Major Vascular Surgery

Background: Major vascular surgery with aortic cross-clamping is associated with temporary ischaemia of the lower limb due to lack of tissue blood flow. The present study was designed to determine if the short-term changes in cellular metabolism occurring during this situation can be detected by subcutaneous microdialysis. It was also hoped to ascertain if this new technique is useful in the continuous bedside monitoring of metabolism during aortic surgery. Methods: In a controlled clinical study 20 patients undergoing elective aortic surgery were monitored using microdialysis probes that were inserted in the subcutaneous tissue of the left lower limb and a non-ischaemic region (shoulder). Interstitial fluid was obtained and the concentrations of glucose and lactate during lower limb ischaemia and during reperfusion were measured and compared with concentrations observed in fluid obtained from the non-ischaemic control tissue. Results: Circulatory occlusion caused an immediate and significant decrease in the glucose/lactate ratio from 3·1±1·3 to 0·48±0·5 (P<0·05) that returned to preocclusion values within 2 h of commencing reperfusion. Conclusion: We suggest that microdialysis may be used both to assess acute changes in tissue metabolism during ischaemic periods and also to act as an additional tool for the detection of peri-operative acute variations in limb blood flow.

Deuterium MR Spectroscopy at 4.7 T

1995 ◽  
Vol 36 (1) ◽  
pp. 85-91 ◽  
Author(s):  
R. Wirestam ◽  
V. Andrée Larsen ◽  
M. Stubgaard ◽  
C. Thomsen ◽  
B. Vikhoff ◽  
...  
Keyword(s):  
Blood Flow ◽  
Mr Spectroscopy ◽  
Normal Tissue ◽  
Subcutaneous Tissue ◽  
Volume Effect ◽  
Tissue Blood Flow ◽  
Injection Technique ◽  
Vasoactive Agents ◽  
Flow Measurements ◽  
Injection Volume

Deuterium MR spectroscopy was used for the determination of tissue blood flow (TBF). The tracer D2O was injected into the tissue of interest, and tracer washout was followed using a 4.7 T spectroscopy/imaging unit. Normal subcutaneous tissue in rats was studied, as well as tissue influenced by vasoactive agents (papaverine and adrenaline). The vasoactive agents introduced changes of 40% in TBF, compared with normal tissue. Normal tissue measurements were repeated using various D2O injection volumes (5–400 μl). The injection volume 5 μl gave TBF 11.7 ± 2.0 ml/100 g·min (mean ± 1 SD). This value was 40% higher than corresponding values observed at larger injection volumes (200–400 μl). This injection volume effect is probably partly due to a capillary dilution caused by tracer administration, and partly related to the non-physiological deuterium signal decrease observed in dead rats. Blood flow measurements in human colon tumours implanted in nude mice showed a rather poor reproducibility, not improved by the use of a multiple site injection technique.

MEASUREMENT OF BLOOD FLOW WITH FREELY DIFFUSIBLE INDICATORS AS INERT GASES, ANTIPYRINE, LABELLED WATER AND RUBIDIUM

1972 ◽  
Vol 68 (2_Supplb) ◽  
pp. S95-S111 ◽  
Author(s):  
Niels A. Lassen ◽  
Ole Andrée Larsen
Keyword(s):  
Blood Flow ◽  
Flow Measurement ◽  
Capillary Wall ◽  
Inert Gases ◽  
Tissue Blood Flow ◽  
Blood Flow Measurement

ABSTRACT Indicators which freely cross the capillary wall can be used for measurement of tissue blood flow in many different ways. Basically one can distinguish two categories of methods, viz. the ones where the indicator enters the tissue via the inflowing blood and the ones where the indicator is deposited locally in the tissue. The most important methods are briefly described with special emphasis on the theory of blood flow measurement.

Blood flow and high-energy phosphates in microregions of left ventricular subendocardium

1981 ◽  
Vol 240 (5) ◽  
pp. H804-H810 ◽  
Author(s):  
H. D. Kleinert ◽  
H. R. Weiss
Keyword(s):  
Blood Flow ◽  
Linear Relationship ◽  
Tissue Perfusion ◽  
High Energy ◽  
Left Ventricular ◽  
Significant Loss ◽  
Tissue Blood Flow ◽  
High Energy Phosphates ◽  
Tissue Samples ◽  
Heterogeneous Distribution

Blood flow and high-energy phosphate (HEP) content were determined simultaneously in multiple microregions of left ventricular subendocardium in 29 normal anesthetized open-chest rabbits by use of a new micromethod to determine whether a direct linear relationship existed between these parameters. Tissue samples weighed 1-2 mg. ATP and creatine phosphate (CP) content were quantitated in quick-frozen hearts by fluorometry at sites where tissue perfusion was measured by H2 clearance by use of bare-tipped platinum electrodes. A series of validation studies were conducted to ensure that 1) no significant damage to the tissue surrounding the electrode occurred during the period of experimentation and 2) no significant loss of biochemical constituents had occurred due to labile processes during freezing or storage of the tissue. Blood flow, ATP, and CP values averaged 79.1 +/- 24.1 (SD) ml.min-1.100 g-1, 4.9 +/- 1.3 mumol/g tissue, and 8.0 +/- 3.0 mumol/g tissue, respectively, and are similar to those reported in studies using larger tissue samples. Correlation between the heterogeneous distribution of tissue perfusion and HEP revealed no direct linear relationship between these parameters in the normal unstressed rabbit subendocardium.

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