scholarly journals Assessment of stem cell viability in the initial healing period in rabbits with a cranial bone defect according to the type and form of scaffold

2019 ◽  
Vol 49 (4) ◽  
pp. 258 ◽  
Author(s):  
Seung-Hwan Kang ◽  
Jun-Beom Park ◽  
InSoo Kim ◽  
Won Lee ◽  
Heesung Kim
Keyword(s):  
Stem Cell ◽  
Cell Viability ◽  
Bone Defect ◽  
Cranial Bone ◽  
Healing Period

The Effect of PAMAM Dendrimers on Mesenchymal Stem Cell Viability and Differentiation

2012 ◽  
Vol 19 (29) ◽  
pp. 4969-4975 ◽  
Author(s):  
M. Goncalves ◽  
R. Castro ◽  
J. Rodrigues ◽  
H. Tomas
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Viability ◽  
Pamam Dendrimers

scholarly journals Shortening and Improving the Embryonic Stem Cell Test through the Use of Gene Biomarkers of Differentiation

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Andrea C. Romero ◽  
Eugenio Vilanova ◽  
Miguel A. Sogorb
Keyword(s):  
Gene Expression ◽  
Risk Assessment ◽  
Stem Cell ◽  
Cell Viability ◽  
Embryonic Stem Cell ◽  
Total Duration ◽  
Embryonic Stem ◽  
End Points ◽  
Cell Test

The embryonic Stem cell Test (EST) is a validated assay for testing embryotoxicityin vitro. The total duration of this protocol is 10 days, and its main end-point is based on histological determinations. It is suggested that improvements on EST must be focused toward molecular end-points and, if possible, to reduce the total assay duration. Five days of exposure of D3 cells in monolayers under spontaneous differentiation to 50 ng/mL of the strong embryotoxic 5-fluorouracil or to 75 μg/mL of the weak embryotoxic 5,5-diphenylhydeantoin caused between 20 and 74% of reductions in the expression of the following genes:Pnpla6,Afp,Hdac7,Vegfa, andNes. The exposure to 1 mg/mL of nonembryotoxic saccharin only caused statistically significant reductions in the expression ofNes. These exposures reduced cell viability of D3 cells by 15, 28, and 34%. We applied these records to the mathematical discriminating function of the EST method to find that this approach is able to correctly predict the embryotoxicity of all three above-mentioned chemicals. Therefore, this work proposes the possibility of improve EST by reducing its total duration and by introducing gene expression as biomarker of differentiation, which might be very interesting forin vitrorisk assessment embryotoxicity.

Adenoviral overexpression of Lhx2 attenuates cell viability but does not preserve the stem cell like phenotype of hepatic stellate cells

2014 ◽  
Vol 328 (2) ◽  
pp. 429-443 ◽  
Author(s):  
Berit Genz ◽  
Maria Thomas ◽  
Brigitte M. Pützer ◽  
Marcin Siatkowski ◽  
Georg Fuellen ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Viability ◽  
Hepatic Stellate Cells ◽  
Stellate Cells

scholarly journals Three-dimensional printed PLA scaffold and human gingival stem cell-derived extracellular vesicles: a new tool for bone defect repair

2018 ◽  
Vol 9 (1) ◽  
Author(s):  
Francesca Diomede ◽  
Agnese Gugliandolo ◽  
Paolo Cardelli ◽  
Ilaria Merciaro ◽  
Valeria Ettorre ◽  
...  
Keyword(s):  
Stem Cell ◽  
Bone Defect ◽  
Extracellular Vesicles ◽  
Three Dimensional ◽  
Bone Defect Repair ◽  
Defect Repair

scholarly journals A high-strength mineralized collagen bone scaffold for large-sized cranial bone defect repair in sheep

2018 ◽  
Vol 5 (5) ◽  
pp. 283-292 ◽  
Author(s):  
Shuo Wang ◽  
Zhijun Zhao ◽  
Yongdong Yang ◽  
Antonios G Mikos ◽  
Zhiye Qiu ◽  
...  
Keyword(s):  
Bone Defect ◽  
High Strength ◽  
Cranial Bone ◽  
Bone Scaffold ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Mineralized Collagen

scholarly journals Injectable BMP ‐2 gene‐activated scaffold for the repair of cranial bone defect in mice

2020 ◽  
Vol 9 (12) ◽  
pp. 1631-1642
Author(s):  
Kai Sun ◽  
Hang Lin ◽  
Ying Tang ◽  
Shiqi Xiang ◽  
Jingwen Xue ◽  
...  
Keyword(s):  
Bone Defect ◽  
Cranial Bone
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