scholarly journals Injectable BMP ‐2 gene‐activated scaffold for the repair of cranial bone defect in mice

2020 ◽  
Vol 9 (12) ◽  
pp. 1631-1642
Author(s):  
Kai Sun ◽  
Hang Lin ◽  
Ying Tang ◽  
Shiqi Xiang ◽  
Jingwen Xue ◽  
...  
Keyword(s):  
Bone Defect ◽  
Cranial Bone

scholarly journals A high-strength mineralized collagen bone scaffold for large-sized cranial bone defect repair in sheep

2018 ◽  
Vol 5 (5) ◽  
pp. 283-292 ◽  
Author(s):  
Shuo Wang ◽  
Zhijun Zhao ◽  
Yongdong Yang ◽  
Antonios G Mikos ◽  
Zhiye Qiu ◽  
...  
Keyword(s):  
Bone Defect ◽  
High Strength ◽  
Cranial Bone ◽  
Bone Scaffold ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Mineralized Collagen

Bone repair and augmentation using block of sintered bovine-derived anorganic bone graft in cranial bone defect model

2009 ◽  
Vol 20 (4) ◽  
pp. 340-350 ◽  
Author(s):  
Tania Mary Cestari ◽  
José Mauro Granjeiro ◽  
Gerson Francisco de Assis ◽  
Gustavo Pompermaier Garlet ◽  
Rumio Taga
Keyword(s):  
Bone Graft ◽  
Bone Defect ◽  
Bone Repair ◽  
Cranial Bone ◽  
Defect Model

scholarly journals Acalvaria in a Malian girl: a new surviving case

2021 ◽  
Author(s):  
Lassana Cissé ◽  
Salimata Diarra ◽  
Abdoulaye Yalcouyé ◽  
Youlouza Coulibaly ◽  
Abdoulaye Tamega ◽  
...  
Keyword(s):  
Congenital Malformation ◽  
Bone Defect ◽  
Dura Mater ◽  
Cranial Vault ◽  
Cranial Bone ◽  
Facial Dysmorphia

Acalvaria is a rare and lethal congenital malformation characterized by the absence of the cranial vault bones, dura mater and associated muscles with complete cranial content. We report a 5-year-old Malian girl seen at 20 months old for facial dysmorphia, hemiparesis, and a cranial bone defect that improved progressively.

scholarly journals Activation of Mesenchymal Stem Cells Promotes New Bone Formation Within Dentigerous Cyst

2020 ◽  
Author(s):  
Yejia Yu ◽  
Mengyu Li ◽  
Yuqiong Zhou ◽  
Yueqi Shi ◽  
Wenjie Zhang ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Regeneration ◽  
Bone Defect ◽  
Dentigerous Cyst ◽  
Healing Process ◽  
Cell Counting ◽  
Cranial Bone ◽  
Alizarin Red

Abstract Background: Dentigerous cyst (DC) is a bone destructive disease and remains a challenge for clinicians. Marsupialization enables bone to regenerate with capsules maintaining, making it a preferred therapeutic means for DC adjacent to vital anatomical structures. Given that capsules of DC derive from odontogenic epithelium remnants at embryonic stage, we investigated whether there were mesenchymal stem cells (MSCs) located in DC capsules and the role that they played in the bone regeneration after marsupialization.Methods: Samples obtained before and after marsupialization were used for histological detection and cell culture. The stemness of cells isolated from fresh tissues were analyzed by morphology, surface marker and multi-differentiation assays. Comparison of proliferation ability between Am-DCSCs and Bm-DCSCs were evaluated by Cell Counting Kit-8 (CCK-8), fibroblast colony-forming units (CFU-F) and 5’‐ethynyl‐2’‐deoxyuridine (EdU) assay. Their osteogenic capacity in vitro was detected by Alkaline phosphatase (ALP) and Alizarin Red staining (ARS), combined with Real-time polymerase chain reaction (RT-PCR) and immunofluorescence (IF) staining. Subcutaneous ectopic osteogenesis as well as cranial bone defect model in nude mice were performed to detect their bone regeneration and bone defect repair ability.Results: Bone tissue and strong ALP activity were detected in the capsule of DC after marsupialization. Two types of MSCs were isolated from fibrous capsules of DC both before (Bm-DCSCs) and after (Am-DCSCs) marsupialization. These fibroblast-like, colony forming cells expressed MSC markers (CD44+, CD90+, CD31-, CD34-, CD45-), and they could differentiate into osteoblast-, adipocyte- and chondrocyte-like cells under induction. Notably, Am-DCSCs performed better in cell proliferation and self-renewal. Moreover, Am-DCSCs showed greater osteogenic capacity both in vitro and in vivo compared with Bm-DCSCs. Conclusions: There are MSCs residing in capsules of DC, and the cell viability as well as osteogenic capacity of them are largely enhanced after marsupialization. Our findings suggested that MSCs might play a crucial role in the healing process of DC after marsupialization, thus providing new insight into the treatment for DC by promoting the osteogenic differentiation of MSCs inside capsules.

Marine spongin incorporation into Biosilicate® for tissue engineering applications: An in vivo study

2020 ◽  
Vol 35 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Julia Risso Parisi ◽  
Kelly Rossetti Fernandes ◽  
Giovanna Caroline Aparecida do Vale ◽  
Alan de França Santana ◽  
Matheus de Almeida Cruz ◽  
...  
Keyword(s):  
Bone Defect ◽  
Bone Structure ◽  
Bone Fractures ◽  
Biological Effects ◽  
Bone Grafts ◽  
Tissue Growth ◽  
Cranial Bone ◽  
Control Group ◽  
Positive Effects ◽  
Post Surgery

Biomaterials and bone grafts, with the ability of stimulating tissue growth and bone consolidation, have been emerging as very promising strategies to treat bone fractures. Despite its well-known positive effects of biosilicate (BS) on osteogenesis, its use as bone grafts in critical situations such as bone defects of high dimensions or in non-consolidated fractures may not be sufficient to stimulate tissue repair. Consequently, several approaches have been explored to improve the bioactivity of BS. A promising strategy to reach this aim is the inclusion of an organic part, such as collagen, in order to mimic bone structure. Thus, the present study investigated the biological effects of marine spongin (SPG)-enriched BS composites on the process of healing, using a critical experimental model of cranial bone defect in rats. Histopathological and immunohistochemistry analyzes were performed after two and six weeks of implantation to investigate the effects of the material on bone repair (supplemental material-graphical abstract). Histological analysis demonstrated that for both BS and BS/SPG, similar findings were observed, with signs of material degradation, the presence of granulation tissue along the defect area and newly formed bone into the area of the defect. Additionally, histomorphometry showed that the control group presented higher values for Ob.S/BS (%) and for N.Ob/T.Ar (mm2) (six weeks post-surgery) compared to BS/SPG and higher values of N.Ob/T.Ar (mm2) compared to BS (two weeks post-surgery). Moreover, BS showed higher values for OV/TV (%) compared to BS/SPG (six weeks post-surgery). Also, VEGF immunohistochemistry was increased for BS (two weeks post-surgery) and for BS/SPG (six weeks) compared to CG. TGFb immunostaining was higher for BS compared to CG. The results of this study demonstrated that the BS and BS/SPG scaffolds were biocompatible and able to support bone formation in a critical bone defect in rats. Moreover, an increased VEGF immunostaining was observed in BS/SPG.

scholarly journals Porous Poly(ε-caprolactone)–Poly(l-lactic acid) Semi-Interpenetrating Networks as Superior, Defect-Specific Scaffolds with Potential for Cranial Bone Defect Repair

2017 ◽  
Vol 18 (12) ◽  
pp. 4075-4083 ◽  
Author(s):  
Lindsay N. Woodard ◽  
Kevin T. Kmetz ◽  
Abigail A. Roth ◽  
Vanessa M. Page ◽  
Melissa A. Grunlan
Keyword(s):  
Lactic Acid ◽  
Bone Defect ◽  
Cranial Bone ◽  
Interpenetrating Networks ◽  
Bone Defect Repair ◽  
Defect Repair ◽  
Porous Poly
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