scholarly journals Electron Microscopy of Satsuma Dwarf Virus in Host Cells

1972 ◽  
Vol 5 (5) ◽  
Author(s):  
Y. Saito ◽  
H Hibino
Keyword(s):  
Electron Microscopy ◽  
Host Cells ◽  
Dwarf Virus ◽  
Satsuma Dwarf Virus

Satsuma dwarf virus. [Distribution map].

2004 ◽  
Author(s):  
Keyword(s):  
Geographical Distribution ◽  
Poncirus Trifoliata ◽  
Dwarf Virus ◽  
Citrus Unshiu ◽  
Distribution Map ◽  
Trifoliate Orange ◽  
Satsuma Dwarf Virus ◽  
Virus Distribution ◽  
New Distribution ◽  
Citrus Spp

Abstract A new distribution map is provided for Satsuma dwarf virus Viruses: Comoviridae: Nepovirus (tentative species) Hosts: Citrus spp., satsuma (Citrus unshiu), kumquat (Fortunella) and trifoliate orange (Poncirus trifoliata). Information is given on the geographical distribution in ASIA, China, Sichuan, Zhejiang, Iran, Japan, Honshu, Kyushu, Shikoku, North Korea, Korea Republic, Turkey.

scholarly journals Diversity of Properties among Satsuma Dwarf Virus and Related Viruses.

1993 ◽  
Vol 59 (6) ◽  
pp. 642-650 ◽  
Author(s):  
Toru IWANAMI ◽  
Meisaku KOIZUMI ◽  
Hiroyuki IEKI
Keyword(s):  
Dwarf Virus ◽  
Satsuma Dwarf Virus

Electron microscopy of host cells infected with tobacco etch virus

Virology ◽  
1967 ◽  
Vol 33 (1) ◽  
pp. 47-54 ◽  
Author(s):  
Takaharu Hayashi ◽  
Chiaki Matsui
Keyword(s):  
Electron Microscopy ◽  
Tobacco Etch Virus ◽  
Host Cells

A new virus related to Satsuma dwarf virus: the nucleotide sequence of the 3?-terminal regions of Hyuganatsu virus RNAs 1 and 2

2004 ◽  
Vol 149 (7) ◽  
Author(s):  
T. Ito ◽  
T. Iwanami ◽  
H. Ieki ◽  
K. Shimomura ◽  
S. Shimizu ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Dwarf Virus ◽  
Satsuma Dwarf Virus

scholarly journals Isolation of Antimicrobial Genes from Oryza rufipogon Griff by Using a Bacillus subtilis Expression System with Potential Antimicrobial Activities

2020 ◽  
Vol 21 (22) ◽  
pp. 8722
Author(s):  
Jiale Li ◽  
Samiul Islam ◽  
Pengfei Guo ◽  
Xiaoqing Hu ◽  
Wubei Dong
Keyword(s):  
Electron Microscopy ◽  
Bacillus Subtilis ◽  
Antimicrobial Peptides ◽  
Expression System ◽  
Oryza Rufipogon ◽  
Host Cells ◽  
Cdna Libraries ◽  
Potential Candidate ◽  
Oryza Rufipogon Griff ◽  
Bacillus Subtilis Expression

Antimicrobial genes are distributed in all forms of life and provide a primary defensive shield due to their unique broad-spectrum resistance activities. To better isolate these genes, we used the Bacillus subtilis expression system as the host cells to build Oryza rufipogon Griff cDNA libraries and screen potential candidate genes from the library at higher flux using built-in indicator bacteria. We observed that the antimicrobial peptides OrR214 and OrR935 have strong antimicrobial activity against a variety of Gram-positive and Gram-negative bacteria, as well as several fungal pathogens. Owing to their high thermal and enzymatic stabilities, these two peptides can also be used as field biocontrol agents. Furthermore, we also found that the peptide OrR214 (MIC 7.7–10.7 μM) can strongly inhibit bacterial growth compared to polymyxin B (MIC 5–25 μM) and OrR935 (MIC 33–44 μM). The cell flow analysis, reactive oxygen burst, and electron microscopy (scanning and transmission electron microscopy) observations showed that the cell membranes were targeted by peptides OrR214 and OrR935, which revealed the mode of action of bacteriostasis. Moreover, the hemolytic activity, toxicity, and salt sensitivity experiments demonstrated that these two peptides might have the potential to be used for clinical applications. Overall, OrR214 and OrR935 antimicrobial peptides have a high-throughput bacteriostatic activity that acts as a new form of antimicrobial agent and can be used as a raw material in the field of drug development.

scholarly journals Flagellum of Legionella pneumophilaPositively Affects the Early Phase of Infection of Eukaryotic Host Cells

2001 ◽  
Vol 69 (4) ◽  
pp. 2116-2122 ◽  
Author(s):  
Claudia Dietrich ◽  
Klaus Heuner ◽  
Bettina C. Brand ◽  
Jörg Hacker ◽  
Michael Steinert
Keyword(s):  
Electron Microscopy ◽  
Legionella Pneumophila ◽  
Kanamycin Resistance ◽  
Etiologic Agent ◽  
Polyclonal Antiserum ◽  
Host Cells ◽  
Kanamycin Resistance Cassette ◽  
Legionnaires Disease ◽  
Invasion Capacity

ABSTRACT Legionella pneumophila, the etiologic agent of Legionnaires' disease, contains a single, monopolar flagellum which is composed of one major subunit, the FlaA protein. To evaluate the role of the flagellum in the pathogenesis and ecology ofLegionella, the flaA gene of L. pneumophila Corby was mutagenized by introduction of a kanamycin resistance cassette. Immunoblots with antiflagellin-specific polyclonal antiserum, electron microscopy, and motility assays confirmed that the specific flagellar mutant L. pneumophila Corby KH3 was nonflagellated. The redelivery of the intact flaA gene into the chromosome (L. pneumophila Corby CD10) completely restored flagellation and motility. Coculture studies showed that the invasion efficiency of the flaA mutant was moderately reduced in amoebae and severely reduced in HL-60 cells. In contrast, adhesion and the intracellular rate of replication remained unaffected. Taking these results together, we have demonstrated that the flagellum of L. pneumophila positively affects the establishment of infection by facilitating the encounter of the host cell as well as by enhancing the invasion capacity.

scholarly journals Modifications in Tissue and Cell Ultrastructure as Elements of Immunity-Like Reaction in Chenopodium quinoa against Prune Dwarf Virus (PDV)

Cells ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 148 ◽  
Author(s):  
Edmund Kozieł ◽  
Katarzyna Otulak-Kozieł ◽  
Józef J. Bujarski
Keyword(s):  
Vascular Tissue ◽  
Chenopodium Quinoa ◽  
Cell Ultrastructure ◽  
Host Cells ◽  
Dwarf Virus ◽  
Ultrastructural Changes ◽  
Viral Pathogen ◽  
Prune Dwarf Virus ◽  
Systemic Spread ◽  
Specific Reactions

Prune dwarf virus (PDV) is a plant RNA viral pathogen in many orchard trees worldwide. Our knowledge about resistance genes or resistant reactions of plant hosts to PDV is scant. To fill in part of this gap, an aim of this study was to investigate reactions to PDV infection in a model host, Chenopodium quinoa. Our investigations concentrated on morphological and ultrastructural changes after inoculation with PDV strain 0599. It turned out that PDV infection can cause deformations in host cells but also induce changes in the organelles, such as chloroplasts in inoculated leaves. Moreover, we also demonstrated specific reactions/changes, which could be associated with both types of vascular tissue capable of effectively blocking the systemic spread of PDV to upper leaves. Furthermore, the relative amount of virus, P1 protein deposition, and movement protein (MP) gene expression consequently decreased in PDV-inoculated leaves.

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