Evaluation of oxathiapiprolin for the management of grapevine Downy mildew

Abstract Downy mildews are obligate biotrophic oomycete pathogens that cause devastating plant diseases on economically important crops. Plasmopara viticola is the causal agent of grapevine downy mildew, a major disease in vineyards worldwide. We sequenced the genome of Pl. viticola with PacBio long reads and obtained a new 92.94 Mb assembly with high contiguity (359 scaffolds for a N50 of 706.5 kb) due to a better resolution of repeat regions. This assembly presented a high level of gene completeness, recovering 1,592 genes encoding secreted proteins involved in plant–pathogen interactions. Plasmopara viticola had a two-speed genome architecture, with secreted protein-encoding genes preferentially located in gene-sparse, repeat-rich regions and evolving rapidly, as indicated by pairwise dN/dS values. We also used short reads to assemble the genome of Plasmopara muralis, a closely related species infecting grape ivy (Parthenocissus tricuspidata). The lineage-specific proteins identified by comparative genomics analysis included a large proportion of RxLR cytoplasmic effectors and, more generally, genes with high dN/dS values. We identified 270 candidate genes under positive selection, including several genes encoding transporters and components of the RNA machinery potentially involved in host specialization. Finally, the Pl. viticola genome assembly generated here will allow the development of robust population genomics approaches for investigating the mechanisms involved in adaptation to biotic and abiotic selective pressures in this species.

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Climate change and the occurrence of downy mildew in Brazilian grapevines

Pesquisa Agropecuária Brasileira ◽

10.1590/s0100-204x2017000600006 ◽

2017 ◽

Vol 52 (6) ◽

pp. 426-434 ◽

Cited By ~ 3

Author(s):

Francislene Angelotti ◽

Emília Hamada ◽

Edineide Elisa Magalhães ◽

Raquel Ghini ◽

Lucas da Ressureição Garrido ◽

...

Keyword(s):

Climate Change ◽

Latent Period ◽

Downy Mildew ◽

Temporal Distribution ◽

Gas Emission ◽

Grapevine Downy Mildew ◽

Diseased Leaf Area ◽

The Mean ◽

Potential Impact ◽

Logic Functions

Abstract: The objective of this work was to evaluate the potential impact of climate change on the occurrence of grapevine downy mildew in Brazil. Seedlings containing four to six leaves were sprayed with a sporangia suspension containing 105 sporangia per milliliter. After spraying, the seedlings were subjected to temperatures of 26, 28, 29.1, 30.4, and 31.8°C for 24 hours. The percentage of diseased leaf area and the latent period were evaluated. Maps of the geographic and temporal distribution of the disease were made considering the monthly average of the mean air temperature and leaf wetness duration for the reference climate or climate normal (1961-1990) and the future climates (2011-2040, 2041-2070, and 2071-2100), considering the A2 and B1 gas emission scenarios, designed by the Intergovernamental Panel on Climate Change (IPCC). Favorability ranges were set and used in logic functions of the geografical information system (GIS) to generate monthly maps for grapevine downy mildew. Rising temperatures interfered with the grapevine downy mildew infections, reduced the disease severity, and increased the latent period. Future climate scenarios indicate a reduction of favorability of downy mildew in Brazil, with variability in the different grape producing regions.

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Timing of Grape Downy Mildew Onset in Bordeaux Vineyards

Phytopathology ◽

10.1094/phyto-12-17-0412-r ◽

2019 ◽

Vol 109 (5) ◽

pp. 787-795 ◽

Cited By ~ 3

Author(s):

Mathilde Chen ◽

François Brun ◽

Marc Raynal ◽

David Makowski

Keyword(s):

Downy Mildew ◽

Severe Disease ◽

Survival Analyses ◽

Fungicide Treatment ◽

Grapevine Downy Mildew ◽

Local Characteristics ◽

South West ◽

Disease Surveys ◽

Grape Downy Mildew ◽

Regional Disease

Grapevine downy mildew (GDM) is a severe disease of grapevines. Because of the lack of reliable information about the dates of GDM symptom onset, many vine growers begin fungicide treatments early in the season. We evaluate the extent to which such preventive treatments are justified. Observational data for 266 untreated sites for the years between 2010 and 2017 were used to estimate the timing of GDM onset on vines and bunches of grapes in South West France (Bordeaux region) through survival analyses. The onset of GDM was not apparent on vines and bunches before early to mid-May, and the rate of GDM symptom appearance was highly variable across years. Depending on the year, 50% of the plots displayed symptoms between mid-May and late June for vines. For several years, our statistical analysis revealed that the proportion of plots with no symptoms was high in early August on vines (27.5 and 43.7% in 2013 and 2016) and on bunches (between 23 and 79% in 2011, 2013, and 2016). We found a significant effect of the amount of rainfall in spring on the date of symptom appearance. These results indicate that preventive fungicide application is unjustified in many vineyards, and that regional disease surveys should be used to adjust fungicide treatment dates according to local characteristics, in particular according to rainfall conditions in spring.

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Factors affecting zoospore responses towards stomata in hop downy mildew (Pseudoperonospora humuli) including some comparisons with grapevine downy mildew (Plasmopara viticola)

Physiological Plant Pathology ◽

10.1016/0048-4059(73)90013-1 ◽

1973 ◽

Vol 3 (3) ◽

pp. 405-417 ◽

Cited By ~ 31

Author(s):

D.J. Royle ◽

G.G. Thomas

Keyword(s):

Downy Mildew ◽

Plasmopara Viticola ◽

Factors Affecting ◽

Grapevine Downy Mildew ◽

Pseudoperonospora Humuli

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Exploring host-pathogen combinations for compatible and incompatible reactions in grapevine downy mildew

European Journal of Plant Pathology ◽

10.1007/s10658-017-1156-2 ◽

2017 ◽

Vol 149 (1) ◽

pp. 1-10 ◽

Cited By ~ 5

Author(s):

Javier Gómez-Zeledón ◽

Markus Kaiser ◽

Otmar Spring

Keyword(s):

Downy Mildew ◽

Grapevine Downy Mildew ◽

Host Pathogen ◽

Pathogen Combinations

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Isolation of Plasmopara viticola from Grapevine Leaves

Proceedings ◽

10.3390/proceedings2019029034 ◽

2019 ◽

Vol 29 (1) ◽

pp. 34

Author(s):

Camelia Ungureanu ◽

Liliana Cristina Soare ◽

Diana Vizitiu ◽

Mirela Calinescu ◽

Irina Fierascu ◽

...

Keyword(s):

Downy Mildew ◽

Plasmopara Viticola ◽

Grapevine Downy Mildew ◽

Grapevine Leaves ◽

Downy Mildew Disease

In order to test some biofungicides, the isolation of Plasmopara viticola was carried out.Plasmopara viticola is a fungus that causes the grapevine downy mildew disease [...]

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Identification of the First Oomycete Mating-type Locus Sequence in the Grapevine Downy Mildew Pathogen, Plasmopara viticola

Current Biology ◽

10.1016/j.cub.2020.07.057 ◽

2020 ◽

Vol 30 (20) ◽

pp. 3897-3907.e4 ◽

Cited By ~ 4

Author(s):

Yann Dussert ◽

Ludovic Legrand ◽

Isabelle D. Mazet ◽

Carole Couture ◽

Marie-Christine Piron ◽

...

Keyword(s):

Downy Mildew ◽

Mating Type ◽

Plasmopara Viticola ◽

Type Locus ◽

Grapevine Downy Mildew ◽

Mating Type Locus

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Draft Genome Sequence of Plasmopara viticola , the Grapevine Downy Mildew Pathogen

Genome Announcements ◽

10.1128/genomea.00987-16 ◽

2016 ◽

Vol 4 (5) ◽

Cited By ~ 17

Author(s):

Yann Dussert ◽

Jérôme Gouzy ◽

Sylvie Richart-Cervera ◽

Isabelle D. Mazet ◽

Laurent Delière ◽

...

Keyword(s):

Downy Mildew ◽

Genome Sequence ◽

Genome Analysis ◽

Plant Pathogen ◽

Host Resistance ◽

Draft Genome ◽

Plasmopara Viticola ◽

Host Specialization ◽

Grapevine Downy Mildew ◽

Plant Pathogen Interactions

Plasmopara viticola is a biotrophic pathogenic oomycete responsible for grapevine downy mildew. We present here the first draft of the P. viticola genome. Analysis of this sequence will help in understanding plant-pathogen interactions in oomycetes, especially pathogen host specialization and adaptation to host resistance.

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