DEVELOPMENT OF IN VITRO PLANT REGENERATION PROTOCOL OF BANANA (MUSA SP.) CV. GRAND NAINE USING SUCKER EXPLANT

Banana is an important fruit crop belongs to the family Musaceae. This has more demand for it multifarious uses like food, medicinal as well as industrial values. The present study was carried out to develop micropropagation protocol for large scale production of banana cv. Grand naine using sucker explant. Sucker explants were inoculated on Murashige and Skoog’s (1962) (MS) basal medium and MS basal medium supplemented with different types and concentrations and combination of plant growth regulators. Highest mean number of shoots (10.2) per explant having mean shoot length 5.2 cm was observed on MS medium supplemented with 4.0 mg/L BA, 2.0 mg/L Z, 1.0 mg/L NAA, and 3.0 mg/L ADS. For large scale production of shoot, in vitro regenerated shoots were harvested, cut into small pieces and inoculated on the optimum medium for multiple shoot proliferation. In this way, more than thousand numbers of in vitro shoots were regenerated from a single explant at six month of culture. In vitro regenerated shoots were excised and rooted on ½ MS medium supplemented with 1.0 mg/L IBA. Finally in vitro regenerated plants were acclimatized and subsequently transferred to field with zero mortality. This protocol helps to meet the demand of the farmers.

Download Full-text

Biochemical and morpho-anatomical analyses of strawberry vitroplants hyperhydric tissues affected by BA and gelling agents

Revista CERES ◽

10.1590/s0034-737x2013000200002 ◽

2013 ◽

Vol 60 (2) ◽

pp. 152-160 ◽

Cited By ~ 5

Author(s):

Leticia Mascarenhas Pereira Barbosa ◽

Vespasiano Borges de Paiva Neto ◽

Leonardo Lucas Carnevalli Dias ◽

Reginaldo Alves Festucci-Buselli ◽

Rodrigo Sobreira Alexandre ◽

...

Keyword(s):

In Vitro Propagation ◽

Large Scale ◽

Culture Medium ◽

Shoot Proliferation ◽

Cellular Level ◽

Fragaria X Ananassa ◽

Scale Production ◽

Ms Medium ◽

Large Scale Production

In vitro propagation has become an effective practice for large-scale production of strawberry plants. The objective of this study was to evaluate the hyperhydricity and the multiplication capacity of two strawberry varieties (Fragaria x ananassa Duch. 'Dover' and 'Burkley') propagated in vitro. Plants maintained in MS medium supplemented with 1.0 mg L-1 BA were individualized and transferred to the same medium solidified with Agar (6.5 g L-1) or Phytagel® (2.5 g L-1) and BA at different concentrations (0; 0.5; 1.0; 2.0 and 3.0 mg L-1). Biochemical and anatomical analyses were carried out, as well as the analysis of the morphological hyperhydricity characteristics. The analysis of data showed: a) the increase in cytokinin concentration increased hyperhydricity frequency in both varieties; b) at concentrations up to 2.0 mg L-1 BA, the replacement of Agar by Phytagel® induced a higher formation of hyperhydric shoots; and c) the addition of BA induced oxidative stress, which is characterized by increased antioxidant activity and lipid peroxidation, as well as alterations at the cellular level, such as malformation of stomata and epidermal cells. In conclusion, the culture medium containing 0.5 mg L-1 BA solidified with Agar provided lower hyperhydricity percentages in association with higher rates of shoot proliferation in strawberry.

Download Full-text

CLONAL MICROPROPAGATION OF MALE-STERILE ZINNIA ELEGANS

HortScience ◽

10.21273/hortsci.25.9.1124 ◽

1990 ◽

Vol 25 (9) ◽

pp. 1124F-1124

Author(s):

R.B. Rogers ◽

M.A.L. Smith ◽

R. Cowen

Keyword(s):

Large Scale ◽

Basal Medium ◽

Shoot Proliferation ◽

Zinnia Elegans ◽

Scale Production ◽

Male Sterile ◽

Clonal Micropropagation ◽

Large Scale Production ◽

High Humidity Environment

The only method for large scale production of pure hybrid seed in Zinnia elegans involves the use of male sterile individuals. The male sterile trait, however, is a three gene recessive which at best produces only 50% male sterile progeny from seed. Since no method of clonal propagation is available, seed-produced female lines require labor intensive field roguing to insure removal of all normal flowered individuals. Clonal micropropagation was investigated as a means of mass producing male steriles for use as female lines. Sterilization procedures were developed for seed and axillary bud explants. Shoot proliferation media containing various levels of BAP, 2ip, and kinetin were screened using in vitro germinated seedling explants of the inbred line `Orange Starlight'. Microshoots demonstrated a high rooting percentage after 2 weeks on basal medium without growth regulators. Plantlets were easily acclimated in 1 to 2 weeks in a high humidity environment. In vitro derived plants of identified male sterile plants were phenotypically evaluated as to their suitability for use in field production.

Download Full-text

CLONAL MICROPROPAGATION OF MALE-STERILE ZINNIA ELEGANS

HortScience ◽

10.21273/hortsci.25.9.1124f ◽

1990 ◽

Vol 25 (9) ◽

pp. 1124f-1124 ◽

Cited By ~ 2

Author(s):

R.B. Rogers ◽

M.A.L. Smith ◽

R. Cowen

Keyword(s):

Large Scale ◽

Basal Medium ◽

Shoot Proliferation ◽

Zinnia Elegans ◽

Scale Production ◽

Male Sterile ◽

Clonal Micropropagation ◽

Large Scale Production ◽

High Humidity Environment

Download Full-text

Biochemical assessment of in vitro and in vivo culture of Tylophora indica (Burm. F.) Merr

Kathmandu University Journal of Science Engineering and Technology ◽

10.3126/kuset.v6i2.4005 ◽

1970 ◽

Vol 6 (2) ◽

pp. 1-5

Author(s):

Antaryami Kaushik ◽

Chandra Gurnani ◽

Shyam Sunder ◽

Abha Dhingra ◽

Vikram Chimpa

Keyword(s):

Large Scale ◽

Basal Medium ◽

Nodal Segments ◽

Scale Production ◽

Tylophora Indica ◽

Shoot Initiation ◽

Large Scale Production ◽

Chlorophyll Protein

Tylophora indica (Burm. F.) Merr is an endangered plant which can be protected from extinction by its large scale production. Nodal segments of healthy plants are used as explants and cultured on MS Basal medium fortified with different growth regulators. Optimum shoot induction conditions from explants were established. In vitro and in vivo phytochemical test were done by using standard methods for chlorophyll, carbohydrates, proteins, lipids and starch. 3mg/l 2, 4 D showed maximum and success full callus production. Shoot initiation started in 7 days and best shoot regeneration reported with 3 mg/ml BAP in Basal medium. Combination of IBA and NAA in concentration 2 and 4 mg/l respectively proved to be best for root initiation. Concentration of chlorophyll, protein, lipid, carbohydrate, and starch in vitro and in vivo culture are investigated. DOI: 10.3126/kuset.v6i2.4005Kathmandu University Journal of Science, Engineering and Technology Vol.6. No II, November, 2010, pp.1-5

Download Full-text

A competent protocol for large scale production of sugarcane (Saccharum officinarum L.) through meristem culture

Journal of Applied and Natural Science ◽

10.31018/jans.v8i1.761 ◽

2016 ◽

Vol 8 (1) ◽

pp. 128-132 ◽

Cited By ~ 1

Author(s):

J. Udhutha ◽

S. C. Mali ◽

H. A. Sahare

Keyword(s):

Apical Meristem ◽

Large Scale ◽

Shoot Formation ◽

Saccharum Officinarum ◽

Meristem Culture ◽

Scale Production ◽

Ms Medium ◽

Large Scale Production ◽

Micro Propagation

A rapid micro propagation and acclimatization response of two different varieties of sugarcane Co86032 and CoN 04131(Saccharum officinarum L.) was obtained in this study. The shoot apical meristem of different sizes wascultured on Murashige and Skoog medium supplemented with different concentrations and combinations of ben-zylaminopurine and kinetin either alone or in combination with each other alongwith GA3. Best shoot formation response in Co 86032 was obtained on MS medium containing 1.5mg/l BAP while in CoN 04131 the combination of 0.5 mg/l BAP with 0.25 mg/l Kinetin showed best shoot formation response from apical meristem. Meristem of 3.0 mm size proved to be the best size for micropropagation of sugarcane. Excellent multiplication response of In vitro formed shoots was obtained when the concentration of BAP was decreased to 1.0 mg/l in Co 86032and 0.25 mg/l BAP and Kin in CoN 04131 (i.e. 0.25 mg/lBAP + 0.25 mg/l Kinetin. MS medium containing 1.0 mg/l NAA and 2.0 mg/l IBA showed 100% rooting response of In vitro regenerated shoots of both the varieties of sugarcane within eight days of inoculation. Best hardening response was obtained in sand+ soil + pressmud (1:1:1) media.

Download Full-text

Micropropagation of Homalomena pineodora Sulaiman & Boyce (Araceae): a new species from Malaysia

Horticultura Brasileira ◽

10.1590/s0102-05362012000100007 ◽

2012 ◽

Vol 30 (1) ◽

pp. 39-43 ◽

Cited By ~ 2

Author(s):

Christine Stanly ◽

Arvind Bhatt ◽

Baharuddin Sulaiman ◽

Chan Lai Keng

Keyword(s):

Large Scale ◽

Ornamental Plants ◽

Basal Medium ◽

Fluorescent Light ◽

Scale Production ◽

Shoot Cultures ◽

Ms Medium ◽

Plantlet Production ◽

Mother Plants

Homalomena pineodora (family Araceae) is a species found to have impressive foliage characteristics which remain evergreen throughout the year. Therefore, H. pineodora can be grown as an ornamental plant. Generally H. pineodora needs 3-5 years to propagate and multiply. However, the demand for new ornamental plants is increasing worldwide and the quality of planting material is a basic need for boosting productivity. Therefore an efficient micropropagation protocol for large-scale production of H. pineodora was developed. In vitro shoot cultures were initiated from the rhizomatous buds on MS basal medium. The best conditions for propagating H. pineodora was found to be MS medium supplemented with 3% sucrose and 0.5 mg L-1 BA (6-benzyladenine) under 24 h of cool fluorescent light which produced an average of 3.8 shoot per explant. Presence of an auxin was not necessary for plantlet production. Liquid MS medium supplemented with 0.5 mg L-1 BA, enhanced the shoot production of H. pineodora as compared to agar-gelled medium with same composition. All the in vitro plantlets of H. pineodora were successfully acclimatized with 100% survival rate. Scanning electron microscopy confirmed the similarity of leaf microstructures between the in vitro and mother plants of H. pineodora.

Download Full-text

High Frequency Direct Organogenesis, Genetic Homogeneity, Chemical Characterization and Leaf Ultra-Structural Study of Regenerants in Diplocyclos palmatus (L.) C. Jeffrey

Agronomy ◽

10.3390/agronomy11112164 ◽

2021 ◽

Vol 11 (11) ◽

pp. 2164

Author(s):

Anamica Upadhyay ◽

Anwar Shahzad ◽

Zishan Ahmad ◽

Abdulrahman A. Alatar ◽

Gea Guerriero ◽

...

Keyword(s):

Secondary Metabolites ◽

In Vitro Propagation ◽

Large Scale ◽

Basal Medium ◽

Wild Plants ◽

Direct Organogenesis ◽

Naphthaleneacetic Acid ◽

Scale Production ◽

Large Scale Production

Diplocyclos palmatus (L.) C. Jeffrey, commonly referred to as “Shivalingi” or “Lollipop climber” is a valuable medicinal plant with a climbing growth habit used in traditional medicine. It is reputed to have antiarthritic, anti-diabetic properties and to be useful in various skin and reproductive problems. Overexploitation of wild plants and low seed germination have resulted in the decline of the species in the wild. Thus, the present investigation was aimed to establish an effective in vitro propagation procedure for its large-scale production and conservation. Nodal explants, obtained from an established mother plant were grown on MS basal medium augmented with various cytokinins, alone or in combination with auxins, to study the morphogenic response. A maximum of 8.3 shoots/explants with an average shoot length of 7.2 cm were produced after six weeks on MS containing benzylaminopurine 5.0 µM + 1-naphthaleneacetic acid 2.0 µM. After 4 weeks of transfer, microshoots rooted well on a low nutrient medium of ½ MS + 1.0 µM indole-3-butyric acid, with a maximum of 11.0 roots/microshoot and an average root length of 7.4 cm. With an 80% survival rate, the regenerated plantlets were effectively acclimatized to natural conditions. DNA-based molecular markers were used to investigate the genetic uniformity. Scanning Electron Microscopic examination of leaves indicated the adaptation of the plantlets to natural, as evidenced by the formation of normal stomata. Gas chromatography-mass spectrometry analyses of mother and micropropagated plants were performed to identify essential secondary metabolites. The results obtained show that the in vitro propagation system can be adopted for preservation, large-scale production and secondary metabolites’ production in D. palmatus.

Download Full-text

Induction of In Vitro Cultured Masses of Shoot Primordia of Hybrid Statice and Its Cryopreservation by Vitrification

HortScience ◽

10.21273/hortsci.32.2.309 ◽

1997 ◽

Vol 32 (2) ◽

pp. 309-311 ◽

Cited By ~ 5

Author(s):

Toshikazu Matsumoto ◽

Yoji Nako ◽

Chiaki Takahashi ◽

Akira Sakai

Keyword(s):

Large Scale ◽

Shoot Tips ◽

High Rate ◽

Naphthaleneacetic Acid ◽

Scale Production ◽

Ms Medium ◽

Shoot Primordia ◽

Large Scale Production

Bulbous structures consisting of meristematic clumps (designated “shoot primordia”) were induced from a meristematic culture of a hybrid statice (Limonium altaica Mill. × L. caspium Mill., cv. Blue Symphonet). The shoot tips were cultured in 25 mL of liquid 1/2 Murashige & Skoog (MS) medium supplemented with 0.44 μm BA and 0.054 μm NAA and 3% sucrose at pH 5.8 by vertically shaking at 2 rpm on rotating stages (1 m in diameter) at 25 °C. One month after inoculation of shoot tips, numerous small globular structures were formed and propagated vegetatively at a high rate following subculture. Segments of shoot primordia had developed into plantlets 2 weeks after transfer to solidified 1/2 MS medium supplemented with 0.44 μm BA and 1% sucrose. Plantlets successfully acclimated and grew into normal plants in a greenhouse. Cold-hardened, precultured small segments of shoot primordia were successfully cryopreserved in liquid N by vitrification. Vitrified and warmed segments plated on solidified 1/2 MS medium produced shoots about 21 d after plating. Cultured masses of shoot primordia appear promising for large-scale production and cryopreservation of annual and biennial statice. Chemical names used: 6-benzyladenine (BA); 1-naphthaleneacetic acid (NAA).

Download Full-text

In Vitro Micropropagation of the Medicinal Plant Physalis angulata L.

Notulae Scientia Biologicae ◽

10.15835/nsb829817 ◽

2016 ◽

Vol 8 (2) ◽

pp. 161-163

Author(s):

Owk ANIEL KUMAR ◽

Songa RAMESH ◽

Sape SUBBA TATA

Keyword(s):

Large Scale ◽

Leaf Disc ◽

Basal Medium ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

Medicinal Herb ◽

Root Induction ◽

Survival Percentage ◽

Physalis Angulata

Physalis angulata L. is an important medicinal herb. An efficient direct adventitious plant regeneration protocol was developed for large scale propagation using leaf disc as explants. The explants were cultured on MS basal medium supplemented with 0.25-3.0 mg/L 6-benzyl amino purine (BAP) for primary shoot proliferation. Inclusion of indole-3-acetic acid (IAA) and gibberellic acid (GA3) in the culture medium along with BAP promoted a higher rate of shoot multiplication. The maximum number of shoots was produced in MS + BAP (1.0 mg/L) + IAA (0.5 mg/L) + GA3 (0.20 mg/L) after the third subculture. An average of 152.8 ± 0.40 shoots were produced from each leaf disc. For root induction the shootlets were transferred to MS medium supplemented with different concentrations of indole-3-butyric acid (IBA). The highest percentage of root induction was observed in 1.0 mg/L (IBA). Rooted plants were successfully established in the soil after hardening. The survival percentage of rooted plants on soil was found to be 85%. This result will facilitate the conservation and propagation of the important medicinal herb Physalis angulata L.

Download Full-text