scholarly journals AN INVESTIGATION ON CALLUS FORMATION STAGES IN ST. JOHN'S WORT, HYPERICUM PERFORATUM L . , IN CULTURE MEDIUM

2020 ◽  
Vol 21 (Supplement 1) ◽  
Author(s):  
Hassan Zahmatkesh ◽  
Elham Azizi ◽  
Mansoure Kermani ◽  
Rahele Rahbarian

St. John's wort is a valuable medicinal plant that has been used for more than 2000 years and is known globally as one of the best medicines in the context of neurological diseases and skin health. As the leading sciences worldwide, plant tissue culture and micropropagation by genetic engineering methods are important indicators of agricultural biotechnology. The fact that undifferentiated plant cells can potentially turn into a complete plant can lead to the conclusion that a new gateway has been opened for biologists. As such, it considerably accelerates the time for the implementation of breeding programs in comparison to traditional plant breeding methods and allows inter-genera crosses in plants. In addition, other basic applications of tissue culture by plant microsamples include maintenance of genetic reserves, production of virus-free plants, and paying economic and practical attention to the production of haploid plants. The present research briefly investigates on callus formation stages of St. John's wort in culture medium (MS).

2021 ◽  
Vol 21 (Suppliment-1) ◽  
pp. 1626-1630
Author(s):  
Hassan Zahmatkesh ◽  
ElhamAzizi . ◽  
Mansoure Kermani ◽  
Rahele Rahbarian

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
E Sofic ◽  
A Copra Janicijevic ◽  
M Maksimovic ◽  
I Tahirovic ◽  
L Klepo ◽  
...  

1991 ◽  
Vol 31 (4) ◽  
pp. 499 ◽  
Author(s):  
MH Campbell ◽  
BR Milne ◽  
JJ Dellow ◽  
HI Nicol

The effect of type of herbicide and time and rate of application on the reduction in ground cover of St John's wort (Hypericum perforatum L.) was determined at Orange, New South Wales. In January, April, July and November 1988, 8 herbicide treatments including the currently used glyphosate and picloram + 2,4-D were applied to vigorously growing H. perforaturn, and the reduction in percentage ground cover was recorded in December 1989. Ineffective herbicides (a.i./ha) were tebuthiuron, 0.8-6.4 kg; metsulfuron, 5-20 g; and paraquat + diquat, 0.4 + 0.5 kg. The addition of metsulfuron (2.5 g a.i./ha) to glyphosate (0.9 and 1.8 kg a.i./ha) did not increase the effectiveness of the latter. Effective herbicides (kg a.i./ha) were triclopyr + picloram, 0.6 + 0.2; picloram + 2,4-D, 0.2 + 0.8; glyphosate, 1.8; and triclopyr, 1.92. There was a strong trend for these herbicides to be more effective in January and November than in April and July. Based on price, effectiveness and selectivity, triclopyr + picloram would be preferred to the other herbicides for boom and spot spraying, and glyphosate would be the only herbicide suitable for aerial application prior to sowing improved pastures on non-arable land.


2011 ◽  
Vol 35 (2) ◽  
pp. 222-235 ◽  
Author(s):  
DÁNIEL COSSUTA ◽  
TÜNDE VATAI ◽  
MÁRIA BÁTHORI ◽  
JUDIT HOHMANN ◽  
TIBOR KEVE ◽  
...  

2002 ◽  
Vol 56 (2) ◽  
pp. 54-59 ◽  
Author(s):  
Dragan Milenovic ◽  
Vlada Veljkovic ◽  
Branimir Todorovic ◽  
Miomir Stankovic

The extraction of resinoids from St. John's wort (Hypericum perforatum L) was studied in a series of two papers. In the first part, the effects of the operating conditions on the yield of resinoids (total extract) were analyzed, while the mathematical models of extraction kinetics were compared in the second one. The extraction was carried out using an aqueous solution of ethanol (70 and 95 % v/v) at a hydromodulus (plant material to solvent ratio, w/v) of 1:5 or 1:10. The plant material was disintegrated and divided into three fractions (mean particle size: 0.23, 0.57 and 1.05 mm). The temperature was 25, 50 or about 80?C (boiling temperature). A higher yield of resinoids was obtained when the plant material of greater disintegration degree (0.23 mm) was treated with 70% v/v aqueous ethanol solution at higher hydromoduli (1:10) and temperatures (80?C). The effects of the operating factors on the yield of resinoids were estimated by using both the full factorial experimental plan 24 and artificial neuronic networks (ANN) of 3-4-1 topology. Of the two methods, the ANN one was found to be advantageous because of its capability of estimating the yield of resinoids in the whole range of the applied operating conditions.


2006 ◽  
Vol 1 (3) ◽  
pp. 182-186 ◽  
Author(s):  
Ali Kemal Ayan ◽  
Ridvan Kizilkaya . ◽  
Cuneyt Cirak . ◽  
Kudret Kevseroglu .

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