Stock Plant Material of St John’s Wort (Hypericum perforatum L.) Based on Biotechnology

The extraction of resinoids from St. John's wort (Hypericum perforatum L) was studied in a series of two papers. In the first part, the effects of the operating conditions on the yield of resinoids (total extract) were analyzed, while the mathematical models of extraction kinetics were compared in the second one. The extraction was carried out using an aqueous solution of ethanol (70 and 95 % v/v) at a hydromodulus (plant material to solvent ratio, w/v) of 1:5 or 1:10. The plant material was disintegrated and divided into three fractions (mean particle size: 0.23, 0.57 and 1.05 mm). The temperature was 25, 50 or about 80?C (boiling temperature). A higher yield of resinoids was obtained when the plant material of greater disintegration degree (0.23 mm) was treated with 70% v/v aqueous ethanol solution at higher hydromoduli (1:10) and temperatures (80?C). The effects of the operating factors on the yield of resinoids were estimated by using both the full factorial experimental plan 24 and artificial neuronic networks (ANN) of 3-4-1 topology. Of the two methods, the ANN one was found to be advantageous because of its capability of estimating the yield of resinoids in the whole range of the applied operating conditions.

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Solid phase concentration of phenolic compounds from the aqueous medicinal raw plant material extracts on the example of St. John’s wort (Hypericum perforatum L.)

Аналитика и контроль ◽

10.15826/analitika.2018.22.3.013 ◽

2018 ◽

Vol 22 (3) ◽

pp. 303-314

Author(s):

Е. А. Shil'ko ◽

◽

V. V. Milevskaya ◽

Z. A. Temerdashev ◽

N. V. Kiseleva ◽

...

Keyword(s):

Phenolic Compounds ◽

Plant Material ◽

Hypericum Perforatum ◽

Solid Phase ◽

St John’S Wort ◽

St John's Wort ◽

Phase Concentration ◽

Hypericum Perforatum L ◽

Solid Phase Concentration

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The content of heavy metals in the leaves of Hypericum perforatum L. on serpentinite soils in Serbia

Glasnik Sumarskog fakulteta ◽

10.2298/gsf0898143o ◽

2008 ◽

pp. 143-153 ◽

Cited By ~ 3

Author(s):

Dragica Obratov-Petkovic ◽

Ivana Bjedov ◽

Snezana Belanovic

Keyword(s):

Heavy Metals ◽

Plant Material ◽

Hypericum Perforatum ◽

Native Plants ◽

Chemical Elements ◽

St John’S Wort ◽

Nickel Chromium ◽

St John's Wort ◽

Iron Nickel ◽

Hypericum Perforatum L

St John's wort is one of the best known and used medicinal plants. The demands for St John's wort in Serbia is still supplied by the collection of native plants. Therefore it was necessary to examine the concentration of heavy metals in the soil and in plant material on serpentinites and to assess the potential safe harvesting and further utilisation of this plant species. The research was performed on three serpentinite sites in Serbia: Zlatibor, Divcibare and Goc. The main soil types were determined and the contents of 7 chemical elements were analyzed in the soil and in the plant material. It was determined that the soils of all three localities had increased concentrations of nickel, chromium and manganese. The St John's wort plant material (leaves) showed the increased concentrations of iron, nickel and chromium. It was concluded that St John's wort was a tolerant species regarding the heavy metal content, and it was recommended to avoid its harvesting on the investigated localities.

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Determination of Hypericin in St. John's Wort (Hypericum perforatum L.) extracts using HPLC-ED

Planta Medica ◽

10.1055/s-0031-1282174 ◽

2011 ◽

Vol 77 (12) ◽

Author(s):

E Sofic ◽

A Copra Janicijevic ◽

M Maksimovic ◽

I Tahirovic ◽

L Klepo ◽

...

Keyword(s):

Hypericum Perforatum ◽

St John’S Wort ◽

St John's Wort ◽

Hypericum Perforatum L

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The effects of the mixture of betaine, vitamin C, St John's wort (Hypericum perforatum L.), lavender, and Melissa officinalis on performance and some physiological parameters in broiler chickens exposed to heat stress

Poultry Science ◽

10.1016/j.psj.2021.101344 ◽

2021 ◽

pp. 101344

Author(s):

Hamid Reza Behboodi ◽

Asghar Sedaghat ◽

Atefeh Baradaran ◽

Hadi Haghbin Nazarpak

Keyword(s):

Heat Stress ◽

Vitamin C ◽

Broiler Chickens ◽

Hypericum Perforatum ◽

Physiological Parameters ◽

Melissa Officinalis ◽

St John’S Wort ◽

St John's Wort ◽

Hypericum Perforatum L

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Effect of herbicides on St John's wort (Hypericum perforatum L.)

Australian Journal of Experimental Agriculture ◽

10.1071/ea9910499 ◽

1991 ◽

Vol 31 (4) ◽

pp. 499 ◽

Cited By ~ 3

Author(s):

MH Campbell ◽

BR Milne ◽

JJ Dellow ◽

HI Nicol

Keyword(s):

Hypericum Perforatum ◽

New South ◽

Arable Land ◽

Ground Cover ◽

Aerial Application ◽

St John’S Wort ◽

Strong Trend ◽

St John's Wort ◽

South Wales ◽

Hypericum Perforatum L

The effect of type of herbicide and time and rate of application on the reduction in ground cover of St John's wort (Hypericum perforatum L.) was determined at Orange, New South Wales. In January, April, July and November 1988, 8 herbicide treatments including the currently used glyphosate and picloram + 2,4-D were applied to vigorously growing H. perforaturn, and the reduction in percentage ground cover was recorded in December 1989. Ineffective herbicides (a.i./ha) were tebuthiuron, 0.8-6.4 kg; metsulfuron, 5-20 g; and paraquat + diquat, 0.4 + 0.5 kg. The addition of metsulfuron (2.5 g a.i./ha) to glyphosate (0.9 and 1.8 kg a.i./ha) did not increase the effectiveness of the latter. Effective herbicides (kg a.i./ha) were triclopyr + picloram, 0.6 + 0.2; picloram + 2,4-D, 0.2 + 0.8; glyphosate, 1.8; and triclopyr, 1.92. There was a strong trend for these herbicides to be more effective in January and November than in April and July. Based on price, effectiveness and selectivity, triclopyr + picloram would be preferred to the other herbicides for boom and spot spraying, and glyphosate would be the only herbicide suitable for aerial application prior to sowing improved pastures on non-arable land.

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