scholarly journals P1BS.7 - An impedimetric immunosensor based on magnetic graphene nanoribbons modified by boronic acid for sensitive label-free detection of lymphoma cancer cells

2018 ◽  
Author(s):  
P. Hashemi ◽  
A. Afkhami ◽  
H. Bagheri
2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
JingJing Fu ◽  
ZhuanZhuan Shi ◽  
Man Li ◽  
Yangyang Wang ◽  
Ling Yu

The chondroitin sulphate proteoglycan 4 (CSPG4), also known as high molecular weight-melanoma associated antigen (HMW-MAA), is a tumor-associated antigen that is expressed in more than 85% of surgically removed melanoma lesions but has restricted distribution in normal tissues. The diagnostic and therapeutic value of CSPG4 drives a need for sensitive and low-cost detection approaches. To this end, we developed a polyaniline/graphene oxide nanocomposite (PANI@GO) that was electrochemically codeposited on indium tin oxide (ITO) electrode. Glutaraldehyde mediated the covalent immobilization of CSPG4 specific antibody mAbD2.8.5 to construct a CSPG4 immunosensor using cell culture media and cell lysate as samples. The fully assembled impedimetric immunosensor was used to detect CSPG4 in CSPG4-positive cell lines M14/CSPG4 and MV3. No impedance signal changes could be observed from CSPG4-negative cell lines M14 and mAbMk2-23 showing the specificity of the CSPG4-impedimetric immunosensor. This low-cost, simple, and label-free analytical method is an alternative to enzyme-linked immunosorbent assay and flow cytometry in screening of CSPG4 in complex biological samples.


2007 ◽  
Vol 23 (4) ◽  
pp. 549-555 ◽  
Author(s):  
Rodica E. Ionescu ◽  
Nicole Jaffrezic-Renault ◽  
Laurent Bouffier ◽  
Chantal Gondran ◽  
Serge Cosnier ◽  
...  

2018 ◽  
Vol 29 (36) ◽  
pp. 365503 ◽  
Author(s):  
Shuyan Zhu ◽  
Hualin Li ◽  
Mengsu Yang ◽  
Stella W Pang

Biosensors ◽  
2016 ◽  
Vol 6 (1) ◽  
pp. 2 ◽  
Author(s):  
Ophélie Berthuy ◽  
Loïc Blum ◽  
Christophe Marquette

Sign in / Sign up

Export Citation Format

Share Document