Histopathological Assessment for the Effect of Allogenic Stem Cells Based Therapy in Stifle Joint for Rabbit Model of Osteoarthritis

Objective. The aim of this study was to investigate regenerative effects of ultrasound- (US-) guided injection with human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs) and/or polydeoxyribonucleotide (PDRN) injection in a chronic traumatic full-thickness rotator cuff tendon tear (FTRCTT) in a rabbit model. Methods. Rabbits (n=32) were allocated into 4 groups. After a 5 mm sized FTRCTT just proximal to the insertion site on the subscapularis tendon was created by excision, the wound was immediately covered by a silicone tube to prevent natural healing. After 6 weeks, 4 injectants (0.2 mL normal saline, G1-SAL; 0.2 mL PDRN, G2-PDRN; 0.2 mL UCB-MSCs, G3-MSC; and 0.2 mL UCB-MSCs with 0.2 ml PDRN, G4-MSC + PDRN) were injected into the FTRCTT under US guidance. We evaluated gross morphologic changes on all rabbits after sacrifice. Masson’s trichrome, anti-type 1 collagen antibody, bromodeoxyuridine, proliferating cell nuclear antigen, vascular endothelial growth factor, and platelet endothelial cell adhesion molecule stain were performed to evaluate histological changes. Motion analysis was also performed. Results. The gross morphologic mean tendon tear size in G3-MSC and G4-MSC + PDRN was significantly smaller than that in G1-SAL and G2-PDRN (p<0.05). However, there were no significant differences in the tendon tear size between G3-MSC and G4-MSC + PDRN. In G4-MSC + PDRN, newly regenerated collagen type 1 fibers, proliferating cell activity, angiogenesis, walking distance, fast walking time, and mean walking speed were greater than those in the other three groups on histological examination and motion analysis. Conclusions. Coinjection of UCB-MSCs and PDRN was more effective than UCB-MSC injection alone in histological and motion analysis in a rabbit model of chronic traumatic FTRCTT. However, there was no significant difference in gross morphologic change of tendon tear between UCB-MSCs with/without PDRN injection. The results of this study regarding the combination of UCB-MSCs and PDRN are worth additional investigations.

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Derivation and Characterization of EGFP-Labeled Rabbit Limbal Mesenchymal Stem Cells and Their Potential for Research in Regenerative Ophthalmology

Biomedicines ◽

10.3390/biomedicines9091134 ◽

2021 ◽

Vol 9 (9) ◽

pp. 1134

Author(s):

Julia I. Khorolskaya ◽

Daria A. Perepletchikova ◽

Daniel V. Kachkin ◽

Kirill E. Zhurenkov ◽

Elga I. Alexander-Sinkler ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Stem Cell ◽

Fluorescent Protein ◽

Rabbit Model ◽

Stem Cell Markers ◽

Epithelial Stem Cells ◽

Limbal Stem Cell ◽

Green Fluorescent

The development of cell-based approaches to the treatment of various cornea pathologies, including limbal stem cell deficiency (LSCD), is an area of current interest in regenerative biomedicine. In this context, the shortage of donor material is urgent, and limbal mesenchymal stem cells (L-MSCs) may become a promising cell source for the development of these novel approaches, being established mainly within the rabbit model. In this study, we obtained and characterized rabbit L-MSCs and modified them with lentiviral transduction to express the green fluorescent protein EGFP (L-MSCs-EGFP). L-MSCs and L-MSCs-EGFP express not only stem cell markers specific for mesenchymal stem cells but also ABCG2, ABCB5, ALDH3A1, PAX6, and p63a specific for limbal epithelial stem cells (LESCs), as well as various cytokeratins (3/12, 15, 19). L-MSCs-EGFP have been proven to differentiate into adipogenic, osteogenic, and chondrogenic directions, as well as to transdifferentiate into epithelial cells. The possibility of using L-MSCs-EGFP to study the biocompatibility of various scaffolds developed to treat corneal pathologies was demonstrated. L-MSCs-EGFP may become a useful tool for studying regenerative processes occurring during the treatment of various corneal pathologies, including LSCD, with the use of cell-based technologies.

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LUNATE ARTHROPLASTY WITH AUTOLOGOUS MESENCHYMAL STEM CELLS IN A RABBIT MODEL

The Journal of Bone and Joint Surgery (American) ◽

10.2106/00004623-200604000-00009 ◽

2006 ◽

Vol 88 (4) ◽

pp. 744-752

Author(s):

JERRY I. HUANG ◽

MAHIDHAR M. DURBHAKULA ◽

PETER ANGELE ◽

BRIAN JOHNSTONE ◽

JUNG U. YOO

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rabbit Model ◽

Autologous Mesenchymal Stem Cells

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Effectiveness of Adhering Adipose-Derived Stem Cells to Defective Cartilage in Promoting Cartilage Regeneration in a Rabbit Model

Arthroscopy The Journal of Arthroscopic and Related Surgery ◽

10.1016/j.arthro.2019.03.018 ◽

2019 ◽

Vol 35 (9) ◽

pp. 2619-2626 ◽

Cited By ~ 4

Author(s):

Hitoaki Numata ◽

Junsuke Nakase ◽

Takeshi Oshima ◽

Hiroyuki Tsuchiya

Keyword(s):

Stem Cells ◽

Rabbit Model ◽

Cartilage Regeneration ◽

Adipose Derived Stem Cells

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Therapeutic Effects of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells Combined with Cartilage Acellular Matrix Mediated Via Bone Morphogenic Protein 6 in a Rabbit Model of Articular Cruciate Ligament Transection

Stem Cell Reviews and Reports ◽

10.1007/s12015-020-09958-9 ◽

2020 ◽

Vol 16 (3) ◽

pp. 596-611 ◽

Cited By ~ 1

Author(s):

Hyo-Jin Jeon ◽

Kyung-Ae Yoon ◽

Eun Suk An ◽

Tae-Wook Kang ◽

Yun-Beom Sim ◽

...

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Cord Blood ◽

Umbilical Cord Blood ◽

Cruciate Ligament ◽

Rabbit Model ◽

Human Umbilical Cord Blood ◽

Human Umbilical Cord ◽

Therapeutic Effects ◽

Acellular Matrix

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Mesenchymal Stem Cells Exhibit Both a Proinflammatory and Anti-Inflammatory Effect on Saccular Aneurysm Formation in a Rabbit Model

Stem Cells International ◽

10.1155/2019/3618217 ◽

2019 ◽

Vol 2019 ◽

pp. 1-15

Author(s):

Michael B. Avery ◽

Brooke L. Belanger ◽

Amy Bromley ◽

Arindom Sen ◽

Alim P. Mitha

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Rabbit Model ◽

Potential Interaction ◽

Allogenic Bone ◽

Aneurysm Formation ◽

Vehicle Injection ◽

Anti Inflammatory

Several studies have demonstrated a potential interaction between mesenchymal stem cells (MSCs) and saccular aneurysms. In this study, we sought to determine whether allogenic bone marrow-derived MSCs had the ability to prevent aneurysm formation in a known rabbit elastase aneurysm model. MSCs were injected intravenously in experimental rabbits at the time of surgical creation and two weeks postcreation and compared with control rabbits receiving vehicle injection. Angiography was used to compare aneurysm measurements four weeks postcreation, and aneurysms were harvested for histological properties. Serum was collected longitudinally to evaluate cytokine alterations. Serum from control animals was also utilized to perform in vitro tests with MSCs to compare the effect of the serologic environment in animals with and without aneurysms on MSC proliferation and cytokine production. While aneurysm morphometric comparisons revealed no differences, significant cytokine alterations were observed in vitro and in vivo, suggesting both anti-inflammatory and proinflammatory processes were occurring in the presence of MSCs. Histological analyses suggested that tunica intima hyperplasia was inhibited in the presence of MSCs.

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