scholarly journals Novel single nucleotide polymorphisms of <i>GnRHR</i> gene and their association with litter size in goats

2011 ◽  
Vol 54 (6) ◽  
pp. 618-624 ◽  
Author(s):  
G. Li ◽  
H.-P. Wu ◽  
M. Z. Fu ◽  
Z.-Q. Zhou
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Litter Size ◽  
Gonadotropin Releasing Hormone ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Strong Candidate ◽  
Boer Goats ◽  
Gonadotropin Releasing Hormone Receptor ◽  
Strong Candidate Gene

Abstract. In the present study, the polymorphisms of gonadotropin-releasing hormone receptor (GnRHR) gene were analysed as a genetic marker candidate for litter size in 720 individuals from Shaanan goats (SG) and Boer goats (BG). Two alleles (A and C), two observed genotypes (AA and AC), and single nucleotide polymorphisms (SNPs) were detected. The frequencies of alleles A and C in two goat breeds were 0.78–0.82 and 0.18–0.22, respectively. The SNP locus was in Hardy–Weinberg disequilibrium in two goat breeds (P<0.05). In addition, comparisons between the nucleotide sequences of AA and AC genotypes showed one mutation (T>A) at exon 2. The results showed that AA genotype was associated with better litter size in SG and BG breeds. Therefore, these results suggest that GnRHR gene is a strong candidate gene that affects litter size in goats.

scholarly journals Single Nucleotide Polymorphisms in the HIRA Gene Affect Litter Size in Small Tail Han Sheep

Animals ◽  
2018 ◽  
Vol 8 (5) ◽  
pp. 71 ◽  
Author(s):  
Mei Zhou ◽  
Zhangyuan Pan ◽  
Xiaohan Cao ◽  
Xiaofei Guo ◽  
Xiaoyun He ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Litter Size ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Association analysis of sixty‐seven single nucleotide polymorphisms with litter size in Dazu Black goats

2019 ◽  
Vol 51 (1) ◽  
pp. 151-152 ◽  
Author(s):  
Guang‐Xin E ◽  
Dong‐Ke Zhou ◽  
Bai‐Gao Yang ◽  
Xing‐Hai Duan ◽  
Ri‐Su Na ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Association Analysis ◽  
Litter Size ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide

Polymorphisms of LHβ and GnRHR genes and their association with the number of embryos recovered in goats

2014 ◽  
Vol 54 (8) ◽  
pp. 987 ◽  
Author(s):  
M. Z. Fu ◽  
G. Li ◽  
Z. Q. Zhou
Keyword(s):  
Polymerase Chain Reaction ◽  
Single Nucleotide Polymorphisms ◽  
Gene Polymorphism ◽  
Corpus Luteum ◽  
Nucleotide Polymorphisms ◽  
Chain Reaction ◽  
Single Strand Conformational Polymorphism ◽  
Single Nucleotide ◽  
Exon 2 ◽  
Polymerase Chain

The objective of the present study was to explore a predictor of superovulation response on the basis of associations between the number of embryos recovered and gene polymorphism. Variation in the goat LHβ and GnRHR genes was investigated using polymerase chain reaction–single-strand conformational polymorphism and DNA sequencing. Two single nucleotide polymorphisms (SNPs) were identified in the 5′-UTR of LHβ gene (A59C, P1 locus) and in the Exon 2 of GnRHR gene (T177A, P6 locus). At the P1 locus in both breeds, the frequencies of one allele were 0.46 and 0.51, respectively. At the P6 locus, the minor allele frequency was 0.23. Associations of both SNPs with the number of embryos recovered and the corpus luteum number were evaluated in Boer and Shaanbei goat breeds. Association analysis showed that both SNPs had significant (P < 0.05) effects on the number of embryos recovered and corpus luteum number. These results indicate that LHβ and GnRHR genes are potential markers for the number of embryos recovered.

scholarly journals Implications of single nucleotide polymorphisms in CD44 exon 2 for risk of breast cancer

2011 ◽  
Vol 20 (5) ◽  
pp. 396-402 ◽  
Author(s):  
Juhua Zhou ◽  
Prakash S. Nagarkatti ◽  
Yin Zhong ◽  
Jiajia Zhang ◽  
Mitzi Nagarkatti
Keyword(s):  
Breast Cancer ◽  
Single Nucleotide Polymorphisms ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Exon 2

scholarly journals Investigation the polymorphism of gonadotropin releasing hormone receptor gene in Iraq sheep

2017 ◽  
Vol 41 (1) ◽  
pp. 138-144
Author(s):  
Hayder Abdul-Kareem Hasan AL-Mutar
Keyword(s):  
Genetic Marker ◽  
Litter Size ◽  
Hormone Receptor ◽  
Receptor Gene ◽  
Gonadotropin Releasing Hormone ◽  
Nucleotide Polymorphisms ◽  
Releasing Hormone ◽  
Serum Gonadotropin ◽  
Gonadotropin Releasing Hormone Receptor ◽  
Hormone Receptor Gene

     In the present study, the investigation includes the estrus activity after synchronization of estrus by using 20 mg impregnated sponges with Medroxy Progesterone Acetate for 12 days with 400 IU I/M injection of pregnant mare serum gonadotropin 24 hrs. before sponges withdrawal. All 30 Iraqi sheep's showed (100%) estrus after sponge withdrawal. Twin percentage 40% while single percentage 60%. The polymorphisms of gonadotropin-releasing hormone receptor gene were analyzed as a genetic marker in 30 Iraqi sheep's. Two noticed genotypes (GA and GT), and single nucleotide polymorphisms in the exon2 region of gonadotropin-releasing hormone receptor gene as genetic marker and correlated with high litter size after hormonal super ovulation response in sheep. The frequencies of alleles G,A and G,T in Iraqi ovine breeds were 0.50 and 0.50, respectively the outcomes revealed that GA, GT genotype was related with better litter-size in Iraqi ovine breeds. Therefore, these outcomes recommend that gonadotropin-releasing hormone receptor gene is a powerful candidate gene that impacts litter-size in sheep.

scholarly journals Detection of single nucleotide polymorphisms at major prolificacy genes in the Mehraban sheep and association with litter size

2018 ◽  
Vol 18 (3) ◽  
pp. 685-698 ◽  
Author(s):  
Reza Talebi ◽  
Ahmad Ahmadi ◽  
Fazlollah Afraz ◽  
Julien Sarry ◽  
Florent Woloszyn ◽  
...  
Keyword(s):  
Single Nucleotide Polymorphisms ◽  
Litter Size ◽  
Potential Role ◽  
Chromosome 6 ◽  
The Novel ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Hardy Weinberg Equilibrium ◽  
Gdf9 Gene

Abstract The present study aimed to investigate the presence of polymorphisms at four known genes controlling ovine prolificacy i.e. BMP15, GDF9, BMPR1B and B4GALNT2 in a sample of 115 Iranian Mehraban ewes and their association with litter size (LS) and lambs’ birth weight (BW) traits. Using Sanger sequencing of exons and polymorphism specific genotyping, ten SNPs (Single Nucleotide Polymorphisms) were observed in only two genes, GDF9 and BMPR1B. Seven SNPs were found in the GDF9 gene on the chromosome 5. Among them, six were already described in the coding sequence, and a new one (g.41840985C>T) was found in the 3’UTR. In the BMPR1B gene on the chromosome 6, three novel SNPs were detected in the exon 7 (g.29382184G>A; g.29382337G>A and g.29382340G>A). Allelic frequencies were established for six SNPs among the ten identified and they were in Hardy-Weinberg equilibrium. A significant association was found between the novel SNPs found in the exon 7 of BMPR1B and LS. Present results indicate the potential role of the BMPR1B locus in controlling prolificacy of Mehraban sheep and provide genetic markers for further exploitation in selection to improve reproductive efficiency.

Sign in / Sign up

Export Citation Format

Share Document