scholarly journals The isolation and characterization of lipase from Carica papaya latex using zwitterion sodium lauroyl sarcosinate as agent

10.5219/1164 ◽  
2019 ◽  
Vol 13 (1) ◽  
pp. 773-778
Author(s):  
Dang Minh Nhat ◽  
Phan Thi Viet Ha
Keyword(s):  
Molecular Structure ◽  
Ammonium Sulphate ◽  
Lipase Activity ◽  
Freeze Drying ◽  
Carica Papaya ◽  
Papaya Latex ◽  
Carica Papaya Latex ◽  
Lauroyl Sarcosinate ◽  
Sodium Lauroyl Sarcosinate

Most of industrial lipases are derived from microbial sources, following by a wide variety of plants. Among plant lipases, lipase from Carica papaya latex has been the focus of intense and growing research due to low cost, easy acceptance by consumers and its unique characteristics. This enzyme has been successfully applied for lipid modification and synthesis of some organic compounds. However, research for its molecular structure has been limited due to the difficulty to isolate the enzyme from the latex matrix. In this study, we suggested a modified approach using sodium lauroyl sarcosinate to solubilize the latex, then the protein was precipitated by ammonium sulphate. We also carried out the characterization of the lipase obtained from Carica papaya latex. The results showed that freeze-drying the fresh latex could improve significantly lipase activity of latex powder in comparison with sun-drying or oven-drying. The zwitterion sodium lauroyl sarcosinate could solubilize nearly 50% of the latex and the achieved supernatant exhibited great lipase activity. There was no need to use an organic solvent to delipidate the latex prior to solubilization with sodium lauroyl sarcosinate due to possible denaturation of enzymes. The proteins which were fractionally precipitated with 50 – 60%, 60 – 70% and 70 – 80% ammonium sulphate saturation showed lipolytic activity. The fraction from 50 – 60% saturation with the greatest mass was subjected to ion exchange chromatography, SDS electrophoresis and kinetic parameter determination. The results showed the presence of two proteins with molecular mass ranging from 35 kDa to 55 kDa and both presented lipase activity. The Km and Vmax of the lipase fraction from 50 – 60% saturation was 1.12 mM and 1.2 x 10-6 mM.min-1.mL-1 respectively. So, the freeze-drying of papaya latex could help to preserve its lipase activity and the usage of sodium lauroyl sarcosinate could improve the isolation of the lipase from the papaya latex and pave the way for research on the molecular structure of Carica papaya latex lipases.

scholarly journals PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya

2019 ◽  
Vol 4 (2) ◽  
pp. 152
Author(s):  
Dwi Putri Mashfufatur Rohmah ◽  
Sofijan Hadi ◽  
Afaf Baktir
Keyword(s):  
Ammonium Sulphate ◽  
Carica Papaya ◽  
Specific Activity ◽  
Total Activity ◽  
Partial Purification ◽  
Purification Factor ◽  
Papaya Latex ◽  
Carica Papaya Latex

AbstractThis research has done partial purification by fractionation and optimization crystallization of papain from Carica papaya latex with the addition of ammonium sulphate. The enhancement of purification factor was determined by its specific activity. The fractionation results show that papain fraction of Carica papaya can be obtained by adding 40-80% saturated ammonium sulphate, with the highest specific activity, i.e. 2,0819 U/µg and the factor purification increase of 6,27 fold than papain extract. Meanwhile, the highest total activity, i.e. 10,7780 U of papain crystals can be obtained by presipitant agent addition of ammonium sulphate in the level / concentration 80% saturated at 15 °C. Microscopycally papain crystals profile in this condition have cube and tetragonal shape.Key words: crystallization, fractionation, ammonium sulphate, papain

Identification and biochemical characterization of a GDSL-motif carboxylester hydrolase from Carica papaya latex

2009 ◽  
Vol 1791 (11) ◽  
pp. 1048-1056 ◽  
Author(s):  
Slim Abdelkafi ◽  
Hiroyuki Ogata ◽  
Nathalie Barouh ◽  
Benjamin Fouquet ◽  
Régine Lebrun ◽  
...  
Keyword(s):  
Carica Papaya ◽  
Biochemical Characterization ◽  
Papaya Latex ◽  
Carica Papaya Latex

Neutral Lipid Characterization of Non-Water-Soluble Fractions of Carica Papaya Latex

2010 ◽  
Vol 87 (9) ◽  
pp. 987-995 ◽  
Author(s):  
Nathalie Barouh ◽  
Slim Abdelkafi ◽  
Benjamin Fouquet ◽  
Michel Pina ◽  
Frantz Scheirlinckx ◽  
...  
Keyword(s):  
Neutral Lipid ◽  
Carica Papaya ◽  
Water Soluble ◽  
Papaya Latex ◽  
Carica Papaya Latex ◽  
Lipid Characterization ◽  
Soluble Fractions

Preparation and Characterization of Enzymes from Spray-Dried Papaya (Carica papaya) Latex

1978 ◽  
Vol 6 (1) ◽  
pp. 255-258 ◽  
Author(s):  
BALDEV S. BAINES ◽  
TREVOR STUCHBURY ◽  
KEITH BROCKLEHURST
Keyword(s):  
Carica Papaya ◽  
Papaya Latex ◽  
Carica Papaya Latex ◽  
Spray Dried

scholarly journals Hydrolytic properties of Carica papaya latex and latex preparations

1937 ◽  
Vol 31 (11) ◽  
pp. 1926-1933 ◽  
Author(s):  
Max Frankel ◽  
Regina Maimin ◽  
Benjamin Shapiro
Keyword(s):  
Carica Papaya ◽  
Papaya Latex ◽  
Carica Papaya Latex

scholarly journals Physico-Chemical Treatment of a Wastewater Collector Loaded with Metals and Suspension Material by The Solution of Raw Carica papaya Latex

2020 ◽  
pp. 42-49
Author(s):  
Togbe Finagnon Crépin Alexis ◽  
Yete Pélagie ◽  
Yovo Franck ◽  
Wotto Valentin
Keyword(s):  
Suspended Matter ◽  
Water Samples ◽  
Carica Papaya ◽  
Metal Removal ◽  
Tropical Plant ◽  
Iron Copper ◽  
Physico Chemical ◽  
Copper Zinc ◽  
Papaya Latex ◽  
Carica Papaya Latex

The papaya (Carica papaya), of the family Caricaceae, is a tree-like tropical plant, native to Central and South America. Papaya cultivation extends to all warm and humid countries. The present study consists in using the solution of raw Carica papaya latex (SLCP) cultivated in Benin as a natural biodegradable flocculant in a physico-chemical process to treat liquid discharges loaded with metals including iron, copper, zinc, and manganese; and suspended matter, and also to test its efficiency against other products commonly used in wastewater treatment. The tests were carried out on water samples from the wastewater collector that runs through the Agla district in Cotonou. The comparative study with a commonly used flocculant, aluminium sulphate, showed outstanding competitiveness with a high flocculation power for the solution of Carica papaya raw latex. In six water samples from the collector with average concentrations of iron, copper, zinc and manganese of 18.20 mg/L ; 2.50mg/L ; 3.80 mg/L and 3.70 mg/L respectively, the percentages of metal removal exceeded 90% for iron and manganese and around 85% for copper and zinc with the combination FeCl3 + SLCP. The NaOH + SLCP combination showed a very significant effect on the removal of metals (96% reduction) and suspended matter.

scholarly journals MOLECULAR WEIGHT PROFILE OF PROTEASE OF PINEAPPLE (Ananas comosus L.Merr) AND PAPAYA (Carica papaya L.) USING SDS-PAGE METHOD

2017 ◽  
Vol 13 (1) ◽  
pp. 52
Author(s):  
Rizky Arcinthya Rachmania ◽  
Priyo Wahyudi ◽  
Aniza Mutia Wardani ◽  
Dini Rohmatul Insani
Keyword(s):  
Molecular Structure ◽  
Molecular Weight ◽  
Gel Electrophoresis ◽  
Carica Papaya ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Ananas Comosus ◽  
Sds Page ◽  
Papaya Latex ◽  
Pharmaceutical Industries

<p>A group of protease enzymes such as papain and bromelain is able to decipher the molecular structure of the protein into amino acids which will be very useful in many fields, especially in food and pharmaceutical industries. The objective of this study to determine the molecular weight profile of enzyme bromelain from pineapple bark (<em>Ananas comosus</em> L. Merr) and papain (<em>Carica papaya</em> L.) from papaya latex with different varieties using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) method. The precipitation was performed with ammonium sulfate ((NH<sub>4</sub>)<sub>2</sub>SO<sub>4</sub>)) 60 % addition and dialysized using a cellophane tubing with a pore size of 12,000 Dalton. The molecular weight of enzyme solution were determined using SDS-PAGE. The analysis results of molecular weight of enzyme bromelain of Subang and Bogor varieties were not different and were about 30.654 kDa, as well as the molecular weight of enzyme papain and Sukma California varieties were also not different and were about 23.485 kDa. It can be concluded that the different varieties of fruit of pineapple and papaya had no effect on the molecular weight of enzyme papain and bromelain.</p>

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