Antiproliferative and Apoptotic Induction of n-Hexane Fraction of Picria fel-terrae lour. Herbs on T47D Cell Line

Avocado plants are widely used as traditional medicine in Indonesia. This research aimed to determine the cytotoxic and antiproliferation activity of avocado seed extract and fractions on the T47D breast cancer cell line. Extraction was done by maceration method using 96% ethanol, then fractionation was done by liquid-liquid partition using n-hexane, chloroform, and ethyl acetate. Cytotoxic and antiproliferation tests were done by the MTT method. Apoptosis activity was investigated by the double staining method, using acridine orange-propidium iodide as a staining reagent. Results showed that the n-hexane fraction of avocado seed had a cytotoxic activity with IC50 27.9 µg/mL. N-hexane fraction of avocado seed inhibited proliferation and induced apoptosis of T47D cell line.

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Adenosine A1 Receptor Antagonist Up-regulates Casp3 and Stimulates Apoptosis Rate in Breast Cancer Cell Line T47D

International Electronic Journal of Medicine ◽

10.34172/iejm.2020.03 ◽

2020 ◽

Vol 9 (1) ◽

pp. 14-20

Author(s):

Mohammad Zamani Rarani ◽

Fahimeh Zamani Rarani ◽

Ali Valiani ◽

Zeinolabedin Shrifian Dastjerdi ◽

Elias Kargar Abargouei ◽

...

Keyword(s):

Cell Line ◽

Cancer Cell Line ◽

P53 Gene ◽

T47d Cell ◽

Adenosine A1 Receptor ◽

T47d Cells ◽

Apoptosis Rate ◽

Adenosine A1 ◽

T47d Cell Line ◽

A1 Receptor

Background: Adenosine receptor family, especially A1 type is-overexpressed in breast-derived tumor cells and the P53 gene is mutant in some of these cells while the casps gene is of wild type as well. The aim of this study was to evaluate the effect of the A1 receptor function on cell programmed death or proliferation, as well as the relationship between this receptor stimulation/inhibition and caspase 3 (casp3) expression in T47D cell line that has a mutant and non-functional P53 gene. Materials and Methods: The expression of casps3 was measured by real-time polymerase chain reaction and then flow cytometery and MTT assay were used to assess the apoptotic and proliferation cell rate after the treatment of T47D cells with specific agonist N6-cyclopentyladenosine (CPA) and antagonist 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) of this receptor 24, 48, and 72 hours after treatment. Result: Our results indicated that DPCPX significantly induces apoptosis in T47D cells and the rate of survival cell after the reduction of this treatment, especially 72 hours after treatment. Finally, the expression of casp3 was up-regulated by DPCPX treatment, especially in 72 hours while CPA treatment had opposite results (P>0.05). Conclusion: In general, DPCPX could up-regulate casp3 gene expression and subsequently increase the apoptosis rate in T47D cells with casp3 expression without the P53 gene interference. Therefore, adenosine A1 receptor antagonists may be introduced as anti-cancer agents.

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New derivatives of a natural nordentatin

Open Chemistry ◽

10.1515/chem-2020-0149 ◽

2020 ◽

Vol 18 (1) ◽

pp. 890-897 ◽

Cited By ~ 1

Author(s):

Tin Myo Thant ◽

Nanik Siti Aminah ◽

Alfinda Novi Kristanti ◽

Rico Ramadhan ◽

Hnin Thanda Aung ◽

...

Keyword(s):

Cell Line ◽

Parent Compound ◽

T47d Cell ◽

Positive Control ◽

Inhibition Assay ◽

Anticancer Activities ◽

Methanol Fraction ◽

T47d Cell Line ◽

Ft Ir ◽

Derivatives Of

AbstractNew derivatives were obtained from natural nordentatin (1) previously isolated from the methanol fraction of Clausena excavata by an acylation method. Herein, we report ten new pyranocoumarin derivatives 1a–1j. Their structures were elucidated based on UV-vis, FT-IR, NMR, and DART-MS data. The α-glucosidase inhibition and anticancer activities of nordentatin (1) and its derivatives were also evaluated. The α-glucosidase inhibition assay exhibited that the derivatives 1b, 1d, 1e, 1f, 1h, 1i, and 1j possess higher inhibitory activity for α-glucosidase with IC50 values of 1.54, 9.05, 4.87, 20.25, 12.34, 5.67, and 2.43 mM, whereas acarbose was used as the positive control, IC50 = 7.57 mM. All derivatives exhibited a weak cytotoxicity against a cervical cancer (HeLa) cell line with the IC50 between 0.25 and 1.25 mM. They also showed moderate to low growth inhibition of a breast cancer (T47D) cell line with IC50 values between 0.043 and 1.5 mM, but their activity was lower than that of the parent compound, nordentatin (1) (IC50 = 0.041 mM).

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