Medicinal plants are considered modern resources for producing agents that could act as alternatives to antibiotics in
demeanor of antibiotic-resistant bacteria. The aim of the study was to evaluate the chemical composition and
antibacterial activities of essential oil of Foeniculum vulgare (FV) against Pseudomonas aeruginosa and Bacillus
subtilis. Gas chromatography mass spectrometry was done to specify chemical composion. As a screen test to detect
antibacterial properties of the essential oil, agar disk and agar well diffusion methods were employed. Macrobroth tube
test was performed to determinate MIC. The results indicated that the most substance found in FV essential oil was
Trans-anethole (47.41 %), also the essential oil of FV with 0.007 g/ml concentration has prevented P. aeruginosa and
with 0.002 g/ml concentration has prevented B. subtilis from the growth. Thus, the research represents the antibacterial
effects of the medical herb on test P. aeruginosa and B. subtilis. We believe that the article provide support to the
antibacterial properties of the essential oil. The results indicate the fact that the essential oil from the plant can be useful
as medicinal or preservatives composition.