UTERINE MICROBIOME IN HEALTHY FERTILE WOMEN AND IN CASE OF ENDOMETRIUM PATHOLOGY IN MULTIPLE FAILURES OF IN-VITRO-FERTILIZATION PROGRAMS

2021 ◽  
Vol 17 (37) ◽  
pp. 293-308
Author(s):  
Victoria Vladislavovna BARINOVA ◽  
Natalya Borisovna KUZNETSOVA ◽  
Irina Olegovna BUSHTYREVA ◽  
Oksana Stanislavna OKSENYUK ◽  
Alexander Evgenievich SHATALOV
Keyword(s):  
In Vitro Fertilization ◽  
Relative Abundance ◽  
Pregnancy Loss ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
The Mean ◽  
Diagnostic Technologies ◽  
Infertile Patients ◽  
Fertile Women

Background: Thanks to new diagnostic technologies, it is of no doubt that the uterus is no longer considered to be sterile. A disturbed microbiome of endometrium can be a significant reason for preterm birth, infertility, the recurrence of pregnancy loss, and repeated implantation failures in in-vitro-fertilization programs. Aim: Study the endometrial microbiome features in healthy fertile women and in women with multiple in-vitro-fertilization failures. Methods: To assess the differences in the endometrial microbiota of 20 women with infertility and experiencing multiple unsuccessful attempts of in-vitro-fertilization and 15 fertile, healthy women, endometrial microbiome was studied using an NGS of 16S rRNA. Results and Discussion: Lactobacillus (29.4%), Comamonas (16.8%), and Mesorhizobium (6.0%) were the most abundant genera in group of healthy fertile patients, and Lactobacillus (33.3%), Ralstonia (7.9%) and Pediococcus (4.8%) were most abundant in the group of infertile patients. The mean relative abundance of Lactobacillus did not significantly differ between groups and comprised 33.3% in the group of infertile women and 29.4% in healthy fertile women. A considerably higher mean relative abundance of bacteria of the genus Brevundimonas and Ralstonia was recorded in the group of infertile women. The fertile women had a statistically significantly higher mean relative abundance of Acidovorax, Brevibacillus, Caulobacter, Comamonas, Delftia, Distigma, Pseudomonas, Schlegelella, and Thermus. Conclusions: The presented data confirm that endometrium is not a sterile tissue despite long-standing concepts concerning its sterility. Lactobacillus are dominant genera; however, there is no absolute dominance of Lactobacillus over 90%. The mean relative abundance of Lactobacillus in the uterine microbiome in fertile patients and patients with multiple in-vitro-fertilization failures did not differ.

scholarly journals Pregnancy Following Laparoscopy Guided Hysteroscopic Tubal Cannulation in Bilateral Tubal Block After Failed IVF Attempts

2020 ◽  
Vol 02 (04) ◽  
pp. 168-170
Author(s):  
Maruf Siddiqui ◽  
Nusrat Ghafoor ◽  
Rusmila Shamim Abdullah ◽  
Fahmida Naznine ◽  
Vinayak B. Mahajan
Keyword(s):  
In Vitro Fertilization ◽  
False Positive ◽  
Significant Proportion ◽  
Alternative Form ◽  
Healthy Baby ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Infertile Patients

Some infertile patients are diagnosed with unilateral or bilateral tubal block by hysterosalpingography (HSG). Laparoscopy is considered as the next step in its investigation and the tubes may be found patent in some of the cases confirming false positive for the HSG. Patients with confirmed bilateral tubal block (BLTB) have no option but to undergo in-vitro fertilization. IVF is a relatively expensive procedure. Many patients in Bangladesh are unable to proceed with repeated attempts if they fail to conceive in the first cycle. A significant proportion of these infertile women remain childless throughout their lives and become psychologically devastated. Laparoscopy guided hysteroscopic tubal cannulation can offer an alternative form of treatment in these groups of patients. Here we report a case in which BLTB was found both in HSG and laparoscopy. The patient then proceeded on to three unsuccessful embryo transfers. Following this, laparoscopy guided hysteroscopic tubal cannulation was performed and it was possible to successfully recanalize one tube. The patient became pregnant and delivered a healthy baby at term.

scholarly journals Microbiome of the Uterus in Women With Unsuccessful in Vitro Fertilization Attempts

2020 ◽  
Vol 8 (4) ◽  
pp. 423-427
Author(s):  
Barinova Viktoriya Vladislavovna ◽  
Kuznetsova Natalya Borisovna ◽  
Bushtyreva Irina Olegovna ◽  
Sokolova Kristina Mikhailovna ◽  
Polev Dmitry Evgenievich ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Ribosomal Rna ◽  
Relative Abundance ◽  
Massively Parallel Sequencing ◽  
Massively Parallel ◽  
Vitro Fertilization ◽  
Healthy Women ◽  
16S Ribosomal Rna Gene ◽  
The Mean

Objectives: This study aimed to investigate the peculiarities of the uterine microbiome in the case of infertility and repeated in vitro fertilization (IVF) failures. Accordingly, the microbiome of the uteri of 22 women with 2 or more IVF failures (first group) and 20 healthy women (second group) was analyzed in this study. Materials and Methods: The samples of uterine microbiomes were collected 7 days after the luteinizing hormone elevation, which was determined by the urine test. All measures were taken to avoid sample contamination. Finally, the massively parallel sequencing of the 16S ribosomal RNA gene was done in every uterine sample. Results: The analysis of the mean relative abundance of various microorganisms in the uterine microbiome showed that women with infertility had higher microbiological diversity and variability compared to healthy women. Eventually, the mean relative abundance of the Lactobacillus genus comprised 34.4% and 63.0% in the first and second groups, respectively. Conclusion: In general, the presence of Lactobacillus in the uterine microbiome could be considered a favorable factor for good reproductive outcomes and successful IVF programs.

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